Method for measuring nitrite by using reductive derivatization resonance Rayleigh scattering method, and application

A technique of resonant Rayleigh scattering and nitrite, which is applied in the field of physical and chemical inspection and research, and achieves the effects of wide application range, strong selectivity and wide linear range

Inactive Publication Date: 2019-10-01
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the method for the determination of nitrite in samples by RRS method. Therefore, the establishment of a detection method for nitrite by means of RRS technology has certain practical significance.

Method used

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  • Method for measuring nitrite by using reductive derivatization resonance Rayleigh scattering method, and application
  • Method for measuring nitrite by using reductive derivatization resonance Rayleigh scattering method, and application
  • Method for measuring nitrite by using reductive derivatization resonance Rayleigh scattering method, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Determination of nitrite in ham sausage by reduction derivatization resonance Rayleigh scattering method

[0037] 1.1 Main instruments and reagents

[0038] F-2500 fluorescence spectrophotometer, electronic balance;

[0039] Sodium nitrite standard stock solution (200μg / mL): Accurately weigh 0.1000g (100°C 4h) sodium nitrite dried to constant weight in a small beaker, dissolve in water, transfer to a 500mL volumetric flask, and dilute with water to scale, mix well, and store in a 4°C refrigerator for later use. Sodium nitrite standard application solution (5μg / mL): Accurately measure 2.5mL sodium nitrite standard stock solution in a 100mL volumetric flask before use, dilute to the mark with water, and mix well. Potassium iodide standard stock solution (5mg / mL): Accurately weigh 0.5000g of potassium iodide and dissolve it in water after drying at 105°C to constant weight, transfer it to a 100mL volumetric flask, dilute to the mark with water, mix well, and st...

Embodiment 2

[0055] Example 2 Reduction Derivatization Resonance Rayleigh Scattering Determination of Nitrite in Milk Powder

[0056] In this application, the main instruments and reagents used, the drawing of the standard curve and the determination of interfering ions are the same as in Example 1.

[0057] 2.1 Processing of milk powder samples

[0058] Accurately weigh 0.2g of milk powder into a 15mL centrifuge tube, add 1mL of potassium ferrocyanide solution with a concentration of 106g / L and 1mL of zinc acetate solution with a concentration of 220g / L, and dilute to 10mL with triple distilled water. The oscillator was vortexed, after centrifugation at 9000r / min, the supernatant was taken, passed through a 0.22μm filter membrane, the first 1.5mL effluent was discarded, and 3.0mL sample working solution was collected for testing.

[0059] 2.2 Determination of nitrite in milk powder

[0060] Take 3.0mL sample working solution in a 10mL colorimetric tube, add 1.5mL potassium iodide soluti...

Embodiment 3

[0062] Example 3 Derivatization Resonance Rayleigh Scattering Determination of Nitrite in Water

[0063] In this application, the main instruments and reagents used, the drawing of the standard curve and the determination of interfering ions are the same as in Example 1.

[0064] 3.1 Treatment of water samples

[0065] Take 10.0 mL of bottled drinking water, filter them with 0.22 μm microporous membranes, and then pass them through cation exchange resin to obtain the sample working solution.

[0066] 3.2 Determination of nitrite in water

[0067] Take 3.0mL sample working solution in a 10mL colorimetric tube, add 1.5mL potassium iodide solution, 1.0mL hydrochloric acid solution, and 0.8mL methyl violet solution in the colorimetric tube in sequence, dilute to the mark with triple distilled water, and mix well , placed at room temperature for 5 minutes to obtain iodide triion-methyl violet compound;

[0068] Take 3.0 mL of the obtained iodine triion-methyl violet compound in ...

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Abstract

The invention relates to a method for measuring nitrite by using a reductive derivatization resonance Rayleigh scattering method, and belongs to the field of physical and chemical inspection and research. A resonance Rayleigh scattering signal of nitrite is very weak, in the method provided by the invention, the nitrite in a sample is caused to generate a redox reaction with iodide ions to generate iodine elementary substance under acidic conditions, the iodine elementary substance is further combined with excess iodide ions to generate triiodide ions, and a cationic dye is combined with thetriiodide ions to form an ionic association that can significantly enhance the RRS signal of the system, so as to generate a new characteristic RRS spectrum. Under selected conditions, the nitrite concentration has a good linear relationship with an RRS value at 471cm-1 with a range of 0.0015-1.5 mug/mL, and an RRS method for indirectly performing quantitative analysis on nitrite is established accordingly. The method provided by the invention is suitable for the measurement of nitrite in food and environmental samples, and has the advantages of simple operation, rapidity, high sensitivity, good selectivity, and so on.

Description

technical field [0001] The invention belongs to the field of physical and chemical inspection research, relates to a method for measuring nitrite by a reduction derivatization resonance Rayleigh scattering method, and is applicable to the determination of nitrite in complex samples such as food and environment. Background technique [0002] Nitrite is one of the main pathogenic factors of chemical food poisoning. Excessive intake of nitrite will make the normal hemoglobin (Fe 2+ ) is oxidized to methemoglobin (Fe 3+ ), leading to tissue hypoxia, cyanosis, dizziness, lethargy and other symptoms, and nausea, vomiting, abdominal pain, diarrhea and other gastrointestinal irritation symptoms. Moreover, nitrite is easy to further form nitrosamine compounds with strong carcinogenicity. [0003] Although nitrite can be used as a preservative and coloring agent in cured meat products, sauced meat products, smoked, roasted, grilled meat, fried meat, Western-style ham, meat enema, fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/643G01N2021/6417
Inventor 贺锦灿钟子惠白研毋福海
Owner GUANGDONG PHARMA UNIV
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