A deep eutectic solvent microemulsion extraction system and method for extracting astaxanthin

A deep eutectic solvent and microemulsion technology, applied in organic chemistry and other directions, can solve the problems of increasing the extraction process time, the recovery rate is only 66%, and it is not very mature, so as to protect the biological activity, shorten the extraction time, and easy to operate. Effect

Active Publication Date: 2021-04-02
GUANGDONG OCEAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the amount of enzyme is relatively large, and there are many impurities such as protein in astaxanthin
In 2014, Cheong et al. used microbial fermentation to recover astaxanthin from shrimp shell waste, which took a long time and the recovery rate was only 66%.
Shrimp shells are hard and contain more calcium components, and ordinary solvents do not have enough penetration into them, which is one of the reasons for increasing the length of the entire extraction process
[0004] It can be seen that the industry of extracting astaxanthin from shrimp shells in the prior art is not very mature and needs to be improved.

Method used

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  • A deep eutectic solvent microemulsion extraction system and method for extracting astaxanthin
  • A deep eutectic solvent microemulsion extraction system and method for extracting astaxanthin
  • A deep eutectic solvent microemulsion extraction system and method for extracting astaxanthin

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preparation example Construction

[0059] In some embodiments, the preparation method of the deep eutectic solvent includes: mixing according to the molar ratio of each component, heating to a temperature of 70-90° C. until clear and transparent, and cooling to room temperature.

[0060] In some embodiments, the preparation method of the deep eutectic solvent microemulsion extraction system includes: mixing the components in proportion and stirring until uniform.

[0061] The present invention also provides a method for extracting astaxanthin with a deep eutectic solvent microemulsion extraction system, comprising the following operations:

[0062] Prepare deep eutectic solvent microemulsion extraction system;

[0063] adding shrimp shell powder to the deep eutectic solvent microemulsion extraction system, and reacting and extracting;

[0064] Separation of solid and liquid to obtain the supernatant, i.e. astaxanthin extract;

[0065] adding a stripping agent to the supernatant for stripping;

[0066] solid-...

Embodiment 1

[0076] Embodiment 1 Deep eutectic solvent microemulsion extraction system of the present invention

[0077] The deep eutectic solvent formed by lactic acid, glucose and water in a molar ratio of 5:1:3, Tween 60, methanol and cyclohexane were mixed according to different mass ratios, and their phases were observed at 30°C. For statistics of the results, see figure 1 , see particle size distribution figure 2 , the results show that the deep eutectic solvent microemulsion extraction system can be guaranteed to be a uniform microemulsion within the proportioning range of the present invention.

Embodiment 2

[0078] Example 2 Astaxanthin extracted with deep eutectic solvent microemulsion of the present invention and residue analysis

[0079] Comprise following operation (same as embodiment 5):

[0080] S1: Preparation of deep eutectic solvent microemulsion extraction system:

[0081] S11. Prepare a deep eutectic solvent: mix proline and ethylene glycol in a molar ratio of 1:3, heat to 80°C until clear and transparent, cool and let it stand at room temperature;

[0082] S12. Preparation of microemulsion extraction system: mix 10%wt of deep eutectic solvent, 52%wt of surfactant, 13%wt of co-surfactant methanol, and 25%wt of cyclohexane, and stir until uniform.

[0083] S2: Add shrimp shell powder to the deep eutectic solvent microemulsion extraction system prepared in S1 at a solid-to-liquid ratio of 1:20 (g / mL), and extract with 70W ultrasound for 60 minutes;

[0084] S3: Centrifuge the reaction solution of S2 at 7000rpm for 8 minutes to separate the solid and liquid, and the supe...

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Abstract

The invention provides a deep eutectic solvent microemulsion extraction system and a method for extracting astaxanthin. The deep eutectic solvent microemulsion extraction system is prepared from, by mass, 10-50% of a deep eutectic solvent, 45-80% of a compound surfactant and 5-40% of cyclohexane. The method for extracting the astaxanthin comprises the following operation steps of preparing the deep eutectic solvent microemulsion extraction system; adding shrimp shell powder into the deep eutectic solvent microemulsion extraction system, and carrying out a reaction for extraction; conducting solid-liquid separation to obtain a liquid supernatant; adding a back-extractant into the liquid supernatant for back-extraction; conducting solid-liquid separation; cleaning an obtained solid, and thenconducting drying to obtain the astaxanthin powder. The deep eutectic solvent microemulsion extraction system has a high solubility to the astaxanthin, and the structure of the astaxanthin cannot bedamaged; the operation of extracting the astaxanthin from shrimp shells through the deep eutectic solvent microemulsion extraction system is simple, the extraction time is short, and the extraction rate is high.

Description

technical field [0001] The invention relates to the technical field of chemical extraction, in particular to a deep eutectic solvent microemulsion extraction system and a method for extracting astaxanthin. Background technique [0002] The antioxidant activity of astaxanthin has been proven to be 10 times that of zeaxanthin, lutein, canthaxanthin and beta-carotene, and 100 times that of alpha-tocopherol. In addition, astaxanthin quenches a single oxygen molecule and scavenge free radicals inside the cell membrane, thereby resisting lipid oxidation, improving the function of the immune system and the order of gene expression. Not only that, astaxanthin also has significant anti-inflammatory, anti-diabetic, cardiovascular disease prevention, immune regulation and anti-cancer functions. In addition, astaxanthin also has the effect of improving the rhythm regulation and hypoglycemic effect of hepatic metabolic disorder caused by high fat. The superior biological activity and o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 高静周晓纯李婉菁张莉莉
Owner GUANGDONG OCEAN UNIVERSITY
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