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Chitosan hydrogel and its preparation method and application

A technology of chitosan and thiolated chitosan, which is applied in the direction of additive processing, can solve the problems of reduced printability and application range, low mechanical strength and toughness of the colloid, and slow curing speed, and can achieve biodegradation speed. Adjustable, adjustable mechanical strength, fast curing effect

Active Publication Date: 2021-11-26
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most 3D bioprinting hydrogels have problems such as slow curing speed, low mechanical strength and toughness of the colloid or cannot be adjusted, which greatly reduces their printability and application range.

Method used

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  • Chitosan hydrogel and its preparation method and application
  • Chitosan hydrogel and its preparation method and application
  • Chitosan hydrogel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] A method of preparing a chitosan hydrogel comprising: a michael addition reaction of α-β unsaturated acyl chitosan with mercapto chitosan; α-β unsaturated acyl chitosan for: The general formula of thiol-chip chitosan is:

[0023] Among them, R 1 The residue portion of the amino group is removed for the chitosan polymer; R 2 Hydrogen atom, alkyl or alkylene group; 3 Carbonyl, carboxy, ester, amide, alkyl or substituted alkyl; 4 It is an alkylene or substituted alkylene group.

[0024] Chitosan (CS), also known as the acetic acid polysaccharide, is a product of chitin deacetylated, is a wide range of natural polysaccharides. Chitosan is the only basic polysaccharide in nature, and there is a pair of free ammonia nitrogen atoms on the molecular chain, which can bind to a hydrogen, so that the chitosan is a positively charged polyelectrolyte. Chitosan has good biocompatibility, biodegradability, sterilization, etc., is a safe natural polymer polymer. The free amino group on t...

Embodiment 1

[0073] The preparation method of the chitoscharchard gel provided in this embodiment includes:

[0074](1) Accurately refer to the chitosan 1.5 g, soluble in acetic acid having a mass fraction of 0.01%. After substantially dissolved under magnetic stirrer, it is placed in an ultrasonic oscillator for 30 min. Accurately weighted the acid reagent (maleic anhydride) 1.8 g, and 5 ml of acetone was added to completely dissolve. In the chitosan acetic acid solution, the dissolved acylation reagent was slowly slowly added, and the mixture was stirred at a magnetic stirrer for about 20 minutes to make it sufficiently mixed. The mixed uniform liquid was transferred into a 150 ml round bottom flask, placed in a collecting magnetic stirrer, provided temperature 40 ° C, constant temperature heating reaction for 2 h. After the reaction was stopped, three days were dialyzed, and dialysate each time it was changed. Frozen drying for three days to get the MCS product.

[0075] (2) Accurately refe...

Embodiment 2

[0080] The preparation method of the chitoscharchard gel provided in this embodiment includes:

[0081] (1) Accurately refer to the chitosan 1.5 g, soluble in acetic acid having a mass fraction of 0.01%. After substantially dissolved under magnetic stirrer, it is placed in an ultrasonic oscillator for 30 min. Accurately weighted the acrylic reagent (β-methacrylic acid) 1.8 g, and 5 ml of acetone was added to completely dissolve. In the chitosan acetic acid solution, the dissolved acylation reagent was slowly slowly added, and the mixture was stirred at a magnetic stirrer for about 20 minutes to make it sufficiently mixed. The mixed uniform liquid was transferred into a 150 ml round bottom flask, placed in a collecting magnetic stirrer, provided temperature 40 ° C, constant temperature heating reaction for 2 h. After the reaction was stopped, three days were dialyzed, and dialysate each time it was changed. Frozen drying for three days to get the MCS product.

[0082] (2) Accuratel...

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Abstract

The invention relates to the technical field of biomaterials, in particular to a chitosan hydrogel and a preparation method thereof, wherein an α-β unsaturated structure is grafted on the chitosan molecular chain, a sulfhydryl group is grafted on the chitosan molecular chain, By using the above two modified chitosan by Michael addition reaction, the rapid solidification of chitosan hydrogel is realized by chemical cross-linking mechanism, so as to realize the 3D printability of the material, and by changing the concentration of the modified material and grafting ratio to adjust the mechanical strength of chitosan hydrogel. The chitosan hydrogel has fast curing speed, good biocompatibility, adjustable mechanical strength, good stability and adjustable biodegradation speed. The general formula of this chitosan hydrogel is: or wherein, R 1 Residue moiety for removal of amino groups from chitosan; R 2 is a hydrogen atom, an alkyl group or an alkylene group; R 3 is carbonyl, carboxyl, ester, amide, alkyl or substituted alkyl; R 4 is an alkylene or substituted alkylene.

Description

Technical field [0001] The present invention relates to the field of biomaterials, and in particular, to a chitoscharination hydrogel and a preparation method thereof. Background technique [0002] 3D printing, also known as adding materials, causing huge changes in many fields, such as engineering, manufacturing industries, education, medicine. 3D Biostatic Profiles Body Organizations or Tissues of Biocompatibility Materials, Cells, and Auxiliary ingredients into 3D functional activity. Compared to non-biological printing, 3D bio printing is more complex, involving biocompatible materials, cell type, growth, differentiation factors and organizational construction, etc., the choice of materials is critical. [0003] Hydrogel is a class of hydrophilic groups that absorb a large amount of moisture swelling but insoluble in water, due to its composition and structure similar to the extracellular matrix, suitable for adhesion, growth of various cells. , Proliferation and differentiat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L5/08B33Y70/00
CPCB33Y70/00C08J3/075C08J2305/08
Inventor 戴建武陈艳艳储筠黄雷
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI