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Preparing method of water-soluble ocean oligo-polysaccharides

A technology of oligomeric polysaccharides and marine polysaccharides, applied in the marine field, can solve the problems of easily destructive polysaccharides, active groups, poor applicability of enzymatic hydrolysis, energy consumption and high noise, etc., and achieve product uniformity and solubility improvement, crystallinity The effect of reducing and lowering the production cost

Active Publication Date: 2019-11-05
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] At present, the degradation methods of polysaccharides include chemical methods such as acid hydrolysis and oxidative degradation, etc., but the products of acid hydrolysis and oxidative degradation are poor in uniformity, low in yield, easy to generate waste, and easy to destroy active groups in polysaccharides, causing pollution to the environment; Methods such as enzymatic hydrolysis, but enzymatic hydrolysis has poor applicability, is difficult to control, and is expensive and difficult to produce on a large scale; physical methods such as ultrasonic method, microwave method, etc. can reduce the use of organic solvents, but the preparation time is long, the efficiency is low, energy consumption and noisy

Method used

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  • Preparing method of water-soluble ocean oligo-polysaccharides
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  • Preparing method of water-soluble ocean oligo-polysaccharides

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preparation example Construction

[0023] A method for preparing water-soluble marine oligopolysaccharides, comprising: providing high-molecular-weight marine polysaccharides; providing electron flow, and the above-mentioned electron flow degrades the above-mentioned polysaccharides by ionizing radiation; providing a separation and purification process to obtain oligopolysaccharides with excellent water solubility; The molecular weight of the above-mentioned oligopolysaccharide is 1×10 3 -5×10 4 Da. This method uses electron current irradiation to degrade high-molecular-weight polysaccharides, and free radicals are generated in the system to attack glycosidic bonds to break them, so as to realize the fragmentation of high-molecular polysaccharides, achieve the purpose of reducing molecular weight, and obtain low-molecular-weight polysaccharides with excellent biological activity. The method has high reproducibility and controllability, no environmental pollution, no significant change in the main chain structu...

Embodiment 1

[0033] A kind of preparation method of water-soluble marine oligopolysaccharide, its concrete steps are as follows:

[0034] (1) high molecular weight chitosan (weight average molecular weight M W 500,000) dispersed in water to form a water dispersion system with a mass concentration of 15%, then the system was left to stand for 12 hours, and then the container was sealed and placed at a temperature of 30°C, an absorbed dose of 200kGy, and a radiation dose rate of 10kGy / Beta ray irradiation under the condition of min;

[0035] (2) After irradiation, the water dispersion system was left to stand for 2h, centrifuged, and the supernatant was dried in a 60°C oven to obtain powdered low molecular weight chitosan, and the product was prepared into a solution with a concentration of 1%. The molecular weight of chitosan was determined by gel permeation chromatography: the mobile phase used was: 0.12mol CH3COOH / 0.06mol CH3COONa aqueous solution, the column temperature was 35°C, and t...

Embodiment 2

[0037] A kind of preparation method of water-soluble marine oligopolysaccharide, its concrete steps are as follows:

[0038] (1) get 5 parts of high molecular weight chitosan (weight average molecular weight M) of 1g respectively W 500,000) dispersed in water to form a water dispersion system with a mass concentration of 25%, then the system was left to stand for 12 hours, and then the container was sealed and sealed, and placed under the conditions of a temperature of 30°C and a radiation dose rate of 10kGy / min. Beta ray irradiation, and set the absorbed dose of each group to be 20, 40, 80, 160, 200kGy respectively;

[0039] (2) After irradiation, the water dispersion system was left to stand for 2 hours, centrifuged, and the supernatant was dried in an oven at 60°C to obtain powdery low molecular weight chitosan with different molecular weights, which were obtained by multi-angle laser scattering (SEC-MALLS ) to determine the relative molecular weight of the same concentrat...

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Abstract

The invention provides a preparing method of water-soluble ocean oligo-polysaccharides, and belongs to the technical field of oceans. The method comprises the steps of providing ocean polysaccharideswith the high molecular weight; providing electron flow, wherein polysaccharide radiation degradation is performed on polysaccharides through the electron flow; performing separation and purificationto obtain the oligo-polysaccharides with the good water solubility. By adopting electron flow irradiation instead of introducing inorganic salt, the purpose of simplifying the steps is achieved, the repeatability and controllability are good, no environment contamination is caused, the degrading efficiency is high, the degrading effect and product uniformity are good, the water-soluble polysaccharides with the low molecular weight can be obtained under the low absorbent amount, the product crystallinity can be lowered, the water soluble performance is improved, and energy consumption and the production cost can be lowered.

Description

technical field [0001] The invention belongs to the field of marine technology, and in particular relates to a preparation method of water-soluble marine oligopolysaccharides. Background technique [0002] In recent years, people have paid great attention to marine polysaccharides. The sources of marine polysaccharides can be divided into three categories: seaweed polysaccharides, marine animal polysaccharides and marine microbial polysaccharides. Seaweed polysaccharides are divided into fucoidan, red algae polysaccharide, green algae polysaccharide and spirulina polysaccharide. Marine microbial polysaccharides are mainly derived from bacteria and fungi, and can be divided into peptidoglycan, dextran and lipopolysaccharide according to their structure. Marine animal polysaccharides such as chitin, glycosaminoglycans and acidic mucopolysaccharides, etc. [0003] Chitosan, the scientific name is (1,4)-2-amino-2-deoxy-β-D glucan. Chitosan is a deacetylated derivative of chi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C08J3/28C08L5/08
CPCC08B37/003C08J3/28C08J2305/08
Inventor 闻正顺
Owner ZHEJIANG OCEAN UNIV
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