Ordered mesoporous TiO2 and applications in nucleic acid molecule detection

Abstract

The invention discloses ordered mesoporous TiO2 and applications in nucleic acid molecule detection. According to the present invention, the ordered mesoporous TiO2 material is subjected to biomimeticsynthesis by using the special nanostructure of a yeast organism as a template based on the principle of biomineralization, can be used in the preparation of biosensing electrodes from electrode sensing active materials so as to be further used for nucleic acid molecule detection, and has advantages of high selectivity, good repeatability, simple and economical operation, and the like.

Description

technical field [0001] The invention belongs to the technical field of biosensing, in particular to an ordered mesoporous TiO 2 and its application in the detection of nucleic acid molecules. Background technique [0002] Cancer is a major disease that threatens human life and health. According to the latest statistics from the National Cancer Center, about 7 people are diagnosed with cancer every minute. With early diagnosis and early treatment, cancer is easily curable. However, often due to the constraints of detection technology, once cancer is diagnosed, most of them are already in the middle and advanced stages, which brings great difficulties to the treatment of cancer. Therefore, early diagnosis and treatment of cancer is the key to cancer prevention and treatment. [0003] miRNA is a kind of non-coding, endogenous small molecule RNA (17-25 bases), which widely exists in animals and plants, and plays a key role in the occurrence and development of cancer or tumor...

AI Technical Summary

Problems solved by technology

Northern blot hybridization method is time-consuming and labor-intensive, requires a large amount of samples, and requires microgram-level samples. This method has low sensitivity and is difficult to achieve low-abundance miRNA and high-throughput detection of miRNA

Microarray analysis method can realize high-throughput detection of miRNA, but its sensitivity is low, specificity is poor, repeatability is poor, and it is prone to false positive problems, and the detection results need to be confirmed by PAGE / Northern blotting, real-time PCR and other technologies, which increases the operation Complexity and Cost of Detection

Although real-time quantitative PCR (real-time PCR) can sensitively detect low-abundance expressed miRNAs and perform high-throughput screening, it is currently the most commonly used technique for detecting miRNA expression. However, miRNA sequences are short, reverse transcription is difficult, and primer design is difficult. Complex, strict temperature control

In addition, non-specific amplification during PCR can easily lead to false positives

[0005] In recent years, some new miRNA detection methods have emerged, involving technologies such as bioluminescence, enzyme analysis, molecular beacons, deep sequencing, locked nucleic acid, and bridging chain reaction, but these methods still have many insurmountable defects. For example, the specificity and sensitivity are relatively low, and the detection system introduces foreign molecular markers such as fluorescent indicators and enzymes, which not only increases the operation complexity and detection cost, but also introduces foreign errors, so it cannot meet the actual application requirements of miRNA detection.

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