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A kind of Bacillus amyloliquefaciens culture, preparation method and application thereof

A technology of amylolytic spores and cultures, which is applied in the field of animal feed additive preparation, and can solve problems such as unsuitable feeding in summer, reducing the conception rate of sows, and affecting the normal development of pigs, etc.

Active Publication Date: 2021-07-06
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Feeding practice has proved that feeding sows with fresh grains containing rice husks will reduce the quality of sow milk and cause diarrhea in piglets; Symptoms such as rapid heartbeat and vasoconstriction affect the normal development of pigs; in addition, distiller's grains are hot and not suitable for feeding in summer
It can be seen that although fresh distiller's grains are rich in nutrients, they are not suitable for feeding animals directly

Method used

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  • A kind of Bacillus amyloliquefaciens culture, preparation method and application thereof

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preparation example Construction

[0043] A preparation method of bacillus amyloliquefaciens culture, is characterized in that, comprises the steps:

[0044] (1) Preparation of solid-state fermentation base material: pretreated white distiller's grains powder is the main fermentation base material, puffed corn flour is used as carbon source, diamine hydrogen phosphate is used as nitrogen source, and calcium carbonate is used as acidity regulator; wherein the added amount of puffed corn flour is 5-15% (w / w pretreated distiller's grain powder); the addition of diammonium phosphate is 5% (w / w pre-treated distiller's grain powder); the addition of calcium carbonate is 1.5-2.0% (w / w pretreatment distiller's grains powder);

[0045] (2) Preparation of Bacillus amyloliquefaciens seed solution: activating Bacillus amyloliquefaciens with a liquid medium, subcultured to make a seed solution;

[0046] (3) Fermentation: Inoculate the seed solution prepared in (2) into the solid-state fermentation base material in (1), the...

Embodiment 1

[0059] Take 5 kg of fresh distiller's grains, add 15% (w / w distiller's grains) puffed corn flour, 0.5% (w / w distiller's grains) diammonium hydrogen phosphate, and replenish water to prepare a fermentation base material. Spread the fermentation base material in the fermentation workshop, with a stacking thickness of 15cm, adjust the temperature of the fermentation workshop, and raise the temperature of the base material to 35±0.2°C. The activated bacillus amyloliquefaciens is diluted and evenly sprayed on the surface of the fermentation base material according to the inoculation amount of 1% (w / v). During the first 12 hours of the fermentation process, the material was turned every 4 hours, and after 12 hours of fermentation, the material was turned every 2 hours. During the fermentation process, samples were taken every 6 hours to determine the pH. The total fermentation time is 72 hours. After the fermentation is over, the number of viable bacteria of Bacillus amyloliquefacie...

Embodiment 2

[0067] The difference between this embodiment and embodiment 1 is only that 8% (w / w distiller's grains) puffed corn flour is added, and the rest of the steps and parameters are the same as in embodiment 1. After the fermentation, the number of live bacteria of Bacillus amyloliquefaciens can reach 18.1 billion / g by dilution coating plate method. The fermented culture is dried, pulverized, and passed through a 100-mesh sieve. Measure and calculate the crude protein content, soluble protein content and the change rate of crude fiber, and include them in Table 1.

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Abstract

The invention discloses a culture of Bacillus amyloliquefaciens, a preparation method and an application thereof. The number of viable bacteria of the cultured species Bacillus amyloliquefaciens is 15-25 billion / g; Corn flour is used as a carbon source, and diammonium bicarbonate is used as a nitrogen source through solid fermentation; the preparation method of the pretreated distiller's grains includes drying to remove water and alcohol, removing rice husks, and preparing rice husks into biomass carbon and then mixing them with The dried distiller's grains are mixed and expanded together to prepare pretreated distiller's grains powder, and the culture of Bacillus amyloliquefaciens of the present invention is used as an animal feed additive, which can effectively prevent diarrhea and promote the growth rate of animals.

Description

technical field [0001] The invention relates to the technical field of preparation of animal feed additives, in particular to a culture of Bacillus amyloliquefaciens, a preparation method and an application thereof. Background technique [0002] Fresh distiller's grains are the by-products of liquor produced by using grain as the main raw material and using the unique solid-state fermentation and solid-state distillation traditional winemaking process. Since the biological fermentation in the brewing process is mainly to convert the starch in the grain into alcohol, although the nutritional components and the content of the same component in distiller's grains are also different due to the different raw materials and processes used, the components are basically similar. The nutrients in distiller's grains mainly come from bacteria and their metabolic products and bacteria autolysates, except for some raw material residues left by saccharification and incomplete fermentation....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K50/30A23K50/10A23K10/12A23K10/38A23K10/30A23K20/26A23K20/20C12R1/07
CPCA23K10/12A23K10/30A23K10/38A23K20/20A23K20/26A23K50/10A23K50/30C12N1/20Y02P60/87
Inventor 彭楠刘云常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV
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