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Process for high-valued utilization of ginseng flowers

A ginseng flower, high-value technology, applied in the biological field, can solve the problems of affecting the nutritional properties of ginseng flower extract, affecting the extraction efficiency of polysaccharides, affecting the structure and activity of polysaccharides, etc., to improve the extraction rate and in vitro antioxidant capacity, significantly Synergistic effect, strong antioxidant effect

Inactive Publication Date: 2019-11-29
青岛浩大生物科技工程有限责任公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reflux method is a traditional polysaccharide extraction method, which has the disadvantages of long extraction time, high energy consumption, large solvent consumption, low yield and relatively low polysaccharide activity.
In recent years, methods such as flash extraction (HE), ultrasonic extraction (UE), and microwave extraction (ME) have been widely used in the extraction of plant polysaccharides. These methods have the characteristics of short time, small amount of solvent, and high extraction yield. The polysaccharide extraction method not only affects the extraction efficiency of polysaccharides, but also affects the structure and activity of polysaccharides, thereby affecting the nutritional properties of ginseng flower extracts
Establishing an extraction method with high antioxidant and nutritional properties of ginseng flowers is the most important technical drawback in this field

Method used

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  • Process for high-valued utilization of ginseng flowers

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preparation example Construction

[0020] (1) The preparation method of the seed liquid of Aspergillus niger is as follows: Under sterile conditions, the potato medium is used to cultivate at 35-40°C for 24 hours to obtain the seed liquid of Aspergillus niger, and the effective number of viable bacteria in the seed liquid is required to be ≥10 9 cfu / mL.

[0021] (2) The preparation method of Bacillus subtilis seed liquid is as follows: Under sterile conditions, use LB medium to cultivate at 28-32°C for 24 hours to obtain liquid Bacillus seed liquid, and the effective number of viable bacteria in the seed liquid is required to be ≥10 9 cfu / mL.

Embodiment 1

[0023] A process for high-value utilization of ginseng flowers. The specific process steps include:

[0024] (1) Microwave-assisted enzymatic hydrolysis: remove impurities from ginseng flower raw materials, crush them, pass through a 80-150 mesh sieve, then add pure water with a mass ratio of 10 times the raw materials of Chinese herbal medicines, and soak for 50 minutes at 40-60°C; add the raw materials of Chinese herbal medicines Compared with 2.5% cellulase and 1.5% non-starch polysaccharase, extract in microwave for 10 minutes, the microwave power is 250-400W, and then let it stand for more than 30 minutes;

[0025] (2) Aerobic fermentation: Continue to add 10 times pure water to the ginseng flower extract after enzymolysis, and then add 4% glucose, 0.4% ammonium sulfate, 0.15% potassium dihydrogen phosphate, and 0.01% magnesium sulfate to the fermentation liquid , stir evenly; inoculate Aspergillus niger and Bacillus subtilis into the fermentation liquid, the inoculation ...

Embodiment 2

[0028] A process for high-value utilization of ginseng flowers. The specific process steps include:

[0029] (1) Microwave-assisted enzymatic hydrolysis: remove impurities from ginseng flower raw materials, crush them, pass through a 80-150 mesh sieve, then add pure water with a mass ratio of 10 times that of Chinese herbal medicine raw materials, and add licorice with a mass ratio of 3% ginseng flowers. Soak at 60°C for 50 minutes; add 2.5% cellulase and 1.5% non-starch polysaccharase in the mass ratio of Chinese herbal medicine raw materials, and extract with microwave assistance for 10 minutes at a microwave power of 250-400W, then let stand for more than 30 minutes;

[0030] (2) Aerobic fermentation: Continue to add 10 times pure water to the ginseng flower extract after enzymolysis, and then add 4% glucose, 0.4% ammonium sulfate, 0.15% potassium dihydrogen phosphate, and 0.01% magnesium sulfate to the fermentation liquid , stir evenly; inoculate Aspergillus niger and Baci...

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Abstract

The invention discloses a process for high-valued utilization of ginseng flowers, and belongs to the technical field of biology. Ginseng flowers are used as raw materials; a modern biological separation technology, a biological enzymolysis technology, a mixed bacteria microbial fermentation technology and a Chinese herbal medicine compatibility application technology are integrated, a ginseng flower extracting solution is prepared through microwave-assisted enzymolysis and a compound microorganism fermentation process, cellulase and protease are adopted in ultrasonic-assisted enzymolysis, andcompound microorganism fermentation comprises aerobic fermentation and anaerobic fermentation; the ginseng flower polysaccharide extraction rate of the obtained ginseng flower extracting solution is remarkably increased, and the ginseng flower extracting solution has high oxidation resistance; the extracting solution can be subjected to concentration, adsorption drying, alcohol precipitation or resin purification and the like according to application ways, and high-valued utilization in the fields of health care, foods, medicines, fresh keeping and the like is met.

Description

technical field [0001] The invention relates to a process for high-value utilization of ginseng flowers, in particular to a ginseng flower extract liquid with high polysaccharide protein extraction rate and high antioxidant capacity prepared by microbial collaborative fermentation, which belongs to the field of biotechnology. Background technique [0002] Ginseng flower, also known as Shencao flower, is the unopened umbel of Araliaceae Panax ginseng. Harvested during the flower bud period, it is naturally sun-dried. The whole ginseng flower is crisp, easy to crush, has a unique aroma, and has a bitter taste. Each ginseng tree grows to 3 years before blooming. Every year, each ginseng has only one inflorescence. 35 kg of ginseng can only harvest a pound of ginseng flowers. It is artificially collected, sun-dried, and dried. It is the essence of ginseng. It is a natural tonic and a precious medicinal material in the world, known as "green gold". Compendium of Materia Medica...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P39/06A23L3/3472
CPCA23L3/3472A61K36/258A61K2236/19A61P39/06
Inventor 王群梁志勇
Owner 青岛浩大生物科技工程有限责任公司
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