Biological fresh-keeping agent for marine fishes, and preparation and using method of biological fresh-keeping agent
A biological preservative, fish technology, applied in the preservation, application, food science and other directions of food ingredients as antimicrobials, can solve the problems of short rigidity time, short shelf life, spoilage and other problems, achieve good water solubility and inhibit growth. , the effect of extending the shelf life
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Embodiment 1
[0045] Composition of raw materials (parts by weight): 10 parts of salmon skin gelatin, 5 parts of lavender extract, 3 parts of ε-polylysine, 5 parts of wood vinegar, 5 parts of oxalic acid modified vitamin E and 3 parts of tea polyphenol.
[0046] Oxalic acid modified vitamin E is prepared by the following method: add 1.5mol oxalic acid, 0.01mol ferric chloride and 820mL water into the reactor, start stirring, raise the temperature to 150°C, slowly add 1mol vitamin E, and react for 3 hours Afterwards, every 30 minutes, take samples to measure the acid value until the acid value no longer changes, and the reaction is over, add 820mL ethyl acetate, pour it into the reaction solution while it is hot, move it into a separatory funnel together, and extract it with saturated saline for 3 times. The total amount is the amount of ethyl acetate, keep the organic layer, and dry it with anhydrous sodium sulfate, let it stand for 30 minutes, filter, remove the ethyl acetate under reduced ...
Embodiment 2
[0058] Composition of raw materials (parts by weight): 20 parts of salmon skin gelatin, 10 parts of rosemary extract, 7 parts of ε-polylysine, 12 parts of wood vinegar, 15 parts of citric acid modified vitamin E and 7 parts of tea polyphenols .
[0059] Oxalic acid-modified vitamin E is prepared by the following method: add 2mol oxalic acid, 0.03mol ferric chloride and 1640mL water into the reactor, start stirring, raise the temperature to 150°C, slowly add 1mol vitamin E, and react for 5 hours , every 30min, take samples to measure the acid value, until the acid value no longer changes, the reaction is over, add 1640mL ethyl acetate, pour it into the reaction solution while it is hot, move it into a separatory funnel together, and extract it with saturated saline for 3 times, the total The amount is the amount of ethyl acetate, keep the organic layer, and dry it with anhydrous sodium sulfate, let it stand for 30 minutes, filter, remove the ethyl acetate under reduced pressure...
Embodiment 3
[0068] Composition of raw materials (parts by weight): 12 parts of salmon skin gelatin, 6 parts of bergamot extract, 4 parts of ε-polylysine, 7 parts of wood vinegar, 7 parts of citric acid modified vitamin E and 4 parts of tea polyphenols.
[0069] Citric acid modified vitamin E is prepared by the following method: add 2mol citric acid, 0.03mol catalyst and 1.5L water into the reactor, start stirring, raise the temperature to 150°C, slowly add 1mol vitamin E, and react for 5-7h, every Every 30 minutes, take a sample to measure the acid value until the acid value does not change any more. After the reaction is over, filter to obtain the crude product; dissolve the crude product in ethanol at 70°C, and after it is completely dissolved, cool down at a rate of 10°C / h until 0°C, and continue to raise the temperature. Crystallize for 3-4 hours, filter, and dry to obtain citric acid-modified vitamin E.
[0070] Citric acid modified vitamin E has the structure shown in formula III: ...
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