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Alpha-pyrone compound, preparation method and applications thereof, and strains

A compound and pyrone technology, applied in the field of ɑ-pyrone compounds, to achieve the effects of no environmental pollution, short cycle and simple operation process

Active Publication Date: 2019-12-13
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to provide a kind of ɑ-pyrone compound, preparation method, bacterial strain and application, solve the problem of large-scale production of this ɑ-pyrone compound, the method is a biosynthetic method, has no pollution to the environment, and This compound has certain anti-tumor activity, which can promote the discovery of active drug lead compounds

Method used

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  • Alpha-pyrone compound, preparation method and applications thereof, and strains
  • Alpha-pyrone compound, preparation method and applications thereof, and strains
  • Alpha-pyrone compound, preparation method and applications thereof, and strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Isolation, Identification and Preservation of Bacterial Strain D and Bacterial Strain ZZP-R1

[0038] 1. Medium

[0039] The final concentration of the PDB seed medium is composed of: potatoes 200g / L, glucose 20g / L, the solvent is distilled water, and the pH is natural.

[0040] PDA medium is to add 20g / L agar to PDB medium.

[0041] WA medium: agar 20g / L, solvent is distilled water, natural pH.

[0042] LB liquid medium: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, solvent is distilled water, pH7.4.

[0043] LB solid medium is 20g / L agar added to LB medium.

[0044] Sand liquid culture medium: tryptone 10g / L, glucose 40g / L, solvent is distilled water, pH is natural.

[0045] The sand-type solid medium is to add 20g / L agar to the sand-type liquid medium.

[0046] 2. Screening and identification of strain D

[0047] Strain D was isolated from the leaves of the coastal plant A. sinensis in the coastal area of ​​Hangzhou Bay, China. The initi...

Embodiment 2

[0121] Embodiment 2: the preparation of bacterial strain D and bacterial strain ZZP-R1 fermentation medium

[0122] (1) Activation culture: Inoculate strain D and strain ZZP-R1 into PDA slant medium (same as Example 1) respectively, and culture in a 28° C. incubator for 3-4 days to activate the strains.

[0123] (2) Seed culture: pick an inoculation loop thalline from the activated colony and inoculate it into the PDB seed medium (same as Example 1), and culture it on a shaking table at 200rpm and 28°C for 3 days to obtain the seed liquid of bacterial strain D and Strain ZZP-R1 seed solution.

[0124] (3) Fermentation culture: Inoculate the seed liquid of the bacterial strain D and the seed liquid of the bacterial strain ZZP-R1 in step (2) to both sides of the rice culture medium with an inoculation amount of 5% of the volume concentration respectively, and leave a 3 cm wide culture medium uninoculated in the middle , cultured statically at 28°C for 45 days, the two bacteria ...

Embodiment 3

[0125] Embodiment 3: Extraction, separation and identification of α-pyrone compound (I)

[0126] 1. Extraction and separation of α-pyrone compound (I)

[0127] The fermentation product prepared in Example 2 was extracted 3 times with an equal volume of ethyl acetate under the assistance of ultrasound, 40KHz ultrasound for 15min each time, filtered through four layers of gauze to obtain an organic layer, and the combined organic layer was concentrated at 40°C in a low-temperature vacuum until there was no liquid flowed out to obtain 90 g of concentrate. 90 g of the concentrate was dissolved in 100 ml of methanol to obtain a suspension, extracted three times with n-hexane of twice the volume of the suspension to obtain a n-hexane extraction layer and a methanol layer, and then the methanol layer was extracted three times with twice the volume of dichloromethane to obtain The dichloromethane extraction layer was concentrated at 45°C in a low-temperature vacuum until no liquid fl...

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Abstract

The invention discloses an alpha-pyrone compound, a preparation method and applications thereof, and strains, and provides a method for co-culturing two strains of fungi on a rice culture medium. According to the present invention, the yield of the alpha-pyrone compound (I) massively generated through fermentation reaches 4%, and the prepared alpha-pyrone compound (I) has certain antitumor activity, and can be subjected to structural modification at a late stage to prepared the derivative so as to substantially improve the antitumor activity, such that the antitumor drug can be prepared; the compound I has an A549 human lung cancer cell strain inhibition rate of 23.54%; and the alpha-pyrone compound is produced by adopting microbial fermentation, such that the method has characteristics ofsimple operation process, short period, low cost and no environmental pollution.

Description

[0001] (1) Technical field [0002] The invention relates to a α-pyrone compound, a preparation method, a bacterial strain and an application. [0003] (2) Background technology [0004] The discovery of new active drug lead compounds has always been a hot spot for medicinal chemists. When searching for potential drugs from animal and plant resources is far from meeting the needs of human beings for the treatment of diseases, microorganisms (mainly fungi and bacteria) have become an important choice for finding new active natural products due to their fast reproduction and easy cultivation. [0005] Studies have shown that plant endophytes are an important component of the abundant functional products in microorganisms. As the transitional zone between sea and land, coastal areas have a very special living environment for their plants, which not only have common aspects with marine and land plants, but also have unique aspects different from them, such as swamp and salinizatio...

Claims

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Application Information

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IPC IPC(8): C07D493/04A61P35/00C12P17/18C12N1/14C12P39/00C12R1/68C12R1/77
CPCA61P35/00C07D493/04C12P17/181C12P39/00C12N1/145C12R2001/68C12R2001/77
Inventor 章华伟潘瑞华熠陈建伟魏斌王鸿
Owner ZHEJIANG UNIV OF TECH
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