Microbial fermenting agent and application thereof to production of fermented aquatic feed
A microbial starter, aquaculture technology, applied in microbial-based methods, animal feed molding or processing, microorganisms, etc., can solve the problems of indigestible substances that are prone to deteriorating water quality, increase environmental burden, and high costs, and achieve a high cost. It is beneficial to digestion and absorption, improve digestion utilization rate, and reduce the effect of bait coefficient
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Embodiment 1
[0031] Embodiment 1 bacteria powder preparation
[0032] 1, the preparation method of aspergillus niger and aspergillus oryzae powder
[0033] (1) Slant culture: inoculate Aspergillus on PDA slant medium, and cultivate at 28°C for 5 days;
[0034] (2) Expansion culture: get aspergillus from PDA medium and inoculate it on the expansion medium of aspergillus, and cultivate it for 5 days at 28° C. to obtain the first-class strain of aspergillus;
[0035] (3) Preparation of secondary seed liquid: Inoculate the primary strain of Aspergillus into the seed medium of Aspergillus by 5% inoculum, 30° C., 200 r / min, and continuously cultivate for 24 hours to obtain the secondary strain of Aspergillus;
[0036] (4) Fermentation tank fermentation: Inoculate the Aspergillus secondary bacterial classification into the Aspergillus fermentation medium by 5% inoculum, 30 DEG C, 240r / min, continuous cultivation for 28h, to obtain Aspergillus fermentation broth;
[0037] (5) Aspergillus ferment...
Embodiment 2
[0045] A microbial fermenting agent, its components and weight ratio are: 45% of bacillus subtilis, 20% of aspergillus niger, 20% of aspergillus oryzae and 15% of bacillus licheniformis.
[0046] According to the above weight ratio, the bacterial powder of each strain prepared in Embodiment 1 was weighed respectively, and after mixing evenly, the microbial starter of the present invention was obtained, which was named starter 1#.
Embodiment 3
[0048] A microbial fermenting agent, its components and weight ratio are: 27% of bacillus subtilis, 15% of aspergillus niger, 33% of aspergillus oryzae and 25% of bacillus licheniformis.
[0049] The preparation method of described microbial fermentation agent is the same as embodiment 2, named as fermentation agent 2#.
PUM
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