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Microbial fermenting agent and application thereof to production of fermented aquatic feed

A microbial starter, aquaculture technology, applied in microbial-based methods, animal feed molding or processing, microorganisms, etc., can solve the problems of indigestible substances that are prone to deteriorating water quality, increase environmental burden, and high costs, and achieve a high cost. It is beneficial to digestion and absorption, improve digestion utilization rate, and reduce the effect of bait coefficient

Active Publication Date: 2020-01-07
青岛玛斯特生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(1) Green feed includes various terrestrial field weeds, artificially cultivated duckweed, Elodea and other aquatic plants, as well as discarded vegetable petioles, etc., which have always occupied a very important position in traditional fish farming The advantage is that there are many types and wide distribution, rich resources, and easy to get; the disadvantage is that the nutrition is low, the growth of farmed animals is slow, the indigestible matter is easy to damage the water quality, and the disease rate is high
(2) The types of agricultural and sideline products mainly include corn, wheat, and barley of cereals, rapeseed cakes, bean cakes, and soybean meal of cakes, rice bran and bran of bran, etc.; this type of feed is commonly referred to as Concentrated feed, compared with green feed, has increased nutrient content, and is generally suitable for popular breeding species such as grass carp, crucian carp, etc. It broadens the digestive channels of agricultural and sideline products, converts plant-based protein into animal-based protein, and improves the nutritional level. The disadvantage is that the nutrition is not comprehensive enough, relatively simple, and contains anti-nutritional substances
(3) The main types of food scraps include livestock and poultry viscera, distiller's grains, bean dregs, and slops from the catering industry, etc. The former can be used to feed carnivorous fish such as catfish and snakehead, which can partially make up for the shortage of animal-derived feed Insufficient, turn waste into treasure, make the best use of everything, reduce pollution to the surrounding environment, the latter can be used to feed grass carp and omnivorous fish, the disadvantage is that the quality is unstable, easy to deteriorate and cause diseases, and affect the quality of farmed animals
(4) Artificial compound feed refers to the feed produced by industrial processing of various feed materials according to the different types, growth stages and nutritional needs of aquatic animals according to a certain formula ratio; the raw materials include both agricultural and sideline products and fish meal , blood meal, meat and bone meal, silkworm chrysalis powder and other animal-derived raw materials, as well as oil and various nutritional additives, etc., have the advantages of comprehensive nutrition, complete types, convenient use, good palatability, rapid growth, relatively few diseases, and conform to the scale It is the main feed for aquaculture feed at present; the disadvantage is that the feed quality of different manufacturers is different, and the large-scale promotion results in a shortage of feed resources and high costs. In order to reduce costs, a large number of plant-based raw materials are used to reduce the anti-nutritional substances in the feed. Many, affecting the growth and health of farmed animals
(5) Other feeds mainly refer to artificially cultivated and captured feed fish, artemia, fly maggots, earthworms, etc., and then feed them to aquatic animals. The advantages are rich and balanced nutrition, good palatability, and low-value feed animals can be converted into The disadvantages of high-grade breeding species are high cost, increased environmental burden, poor quality control and easy outbreak of disease
At present, the quality of fermented feed starters varies from good to bad. At present, we are paying attention to the total bacterial count and fermentation odor of the starter. Less attention has been paid to the effect of degrading macromolecules and reducing anti-nutritional substances during the fermentation process, and the effect of fermented feed has not been fully utilized. ; Good quality fermented feed can not only improve the nutritional value of the feed, but also contain a large number of beneficial bacteria such as lactic acid bacteria, yeasts, and spores, as well as organic acids, feed anti-nutrient decomposing enzymes, digestive enzymes, etc.

Method used

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  • Microbial fermenting agent and application thereof to production of fermented aquatic feed
  • Microbial fermenting agent and application thereof to production of fermented aquatic feed
  • Microbial fermenting agent and application thereof to production of fermented aquatic feed

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1 bacteria powder preparation

[0032] 1, the preparation method of aspergillus niger and aspergillus oryzae powder

[0033] (1) Slant culture: inoculate Aspergillus on PDA slant medium, and cultivate at 28°C for 5 days;

[0034] (2) Expansion culture: get aspergillus from PDA medium and inoculate it on the expansion medium of aspergillus, and cultivate it for 5 days at 28° C. to obtain the first-class strain of aspergillus;

[0035] (3) Preparation of secondary seed liquid: Inoculate the primary strain of Aspergillus into the seed medium of Aspergillus by 5% inoculum, 30° C., 200 r / min, and continuously cultivate for 24 hours to obtain the secondary strain of Aspergillus;

[0036] (4) Fermentation tank fermentation: Inoculate the Aspergillus secondary bacterial classification into the Aspergillus fermentation medium by 5% inoculum, 30 DEG C, 240r / min, continuous cultivation for 28h, to obtain Aspergillus fermentation broth;

[0037] (5) Aspergillus ferment...

Embodiment 2

[0045] A microbial fermenting agent, its components and weight ratio are: 45% of bacillus subtilis, 20% of aspergillus niger, 20% of aspergillus oryzae and 15% of bacillus licheniformis.

[0046] According to the above weight ratio, the bacterial powder of each strain prepared in Embodiment 1 was weighed respectively, and after mixing evenly, the microbial starter of the present invention was obtained, which was named starter 1#.

Embodiment 3

[0048] A microbial fermenting agent, its components and weight ratio are: 27% of bacillus subtilis, 15% of aspergillus niger, 33% of aspergillus oryzae and 25% of bacillus licheniformis.

[0049] The preparation method of described microbial fermentation agent is the same as embodiment 2, named as fermentation agent 2#.

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Abstract

The invention belongs to the technical field of microbial application, and particularly relates to a microbial fermenting agent and application thereof to production of fermented aquatic feed. According to the fermenting agent, through the synergistic action of various microbial strains, protease and amylase can be produced with the high yield, macromolecular proteins in fermentation raw materialsare fully decomposed into small peptides and amino acids advantageously, and the nutritive value of the fermented feed is improved.

Description

technical field [0001] The invention belongs to the technical field of microbial application, and in particular relates to a microbial starter and its application in the production of aquaculture fermented feed. Background technique [0002] In daily aquaculture production practice, except for silver carp and bighead carp and other filter-feeding fish that can rely on plankton and natural bait such as aquatic plants and snails growing in river ponds, most aquaculture species need artificial The bait is fed to make up for the shortage of natural bait. The type and quality of the feed are directly related to the economic benefits of the breeder, and even the success or failure of the breeding. [0003] According to different sources of feed, it can generally be divided into green feed, agricultural and sideline product feed, food waste, artificial compound feed and other categories. (1) Green feed includes various terrestrial field weeds, artificially cultivated duckweed, El...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/12A23K10/22A23K10/20A23K20/147A23K10/26A23K10/37A23K10/30A23K20/158A23K20/163A23K40/10C12N1/14C12N1/20C12R1/125C12R1/685C12R1/69C12R1/10
CPCA23K50/80A23K10/12A23K10/22A23K10/20A23K20/147A23K10/26A23K10/37A23K10/30A23K20/158A23K20/163A23K40/10C12N1/14C12N1/20Y02P60/87
Inventor 王磊魏万权刘广周怡郭本月王鲁波刘阳
Owner 青岛玛斯特生物技术有限公司