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Application of lactococcus lactis KDLL2016-01 in preparation of protein feed by fermenting feathers

A technology of Lactococcus lactis and protein feed, which is applied in the field of animal nutrition and feed, can solve the problems of livestock and poultry toxicity, large nutrient damage, and surrounding environmental pollution, and achieve low cost, good palatability, and increased feed intake. Effect

Active Publication Date: 2020-01-10
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention aims at the problems that the chemical hydrolysis method and puffing method process feathers as protein feed, which will greatly damage the nutritional components and pollute the surrounding environment, and the secondary metabolites of microbial fermented feathers may have toxicity to livestock and poultry To solve such problems, Lactococcus, one of the probiotics, is used to ferment feathers to produce feather protein feed

Method used

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  • Application of lactococcus lactis KDLL2016-01 in preparation of protein feed by fermenting feathers
  • Application of lactococcus lactis KDLL2016-01 in preparation of protein feed by fermenting feathers
  • Application of lactococcus lactis KDLL2016-01 in preparation of protein feed by fermenting feathers

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Effect test

Embodiment 1

[0040] Example 1: Isolation, screening and identification of feather meal degrading bacteria

[0041] 1. Experimental method

[0042](1) Collection of samples: ① The strain isolation samples were collected from the feces of broiler chickens raised in the animal breeding base of Northeast Agricultural University in 2016; Homogenize to make a suspension, take 1mL of the suspension and put it into a 250mL Erlenmeyer flask containing 100mL of enrichment medium, enrich and cultivate at 30°C for 48h at 220r / min to prepare an enrichment culture solution.

[0043] (2) Preliminary screening: After diluting the enriched culture solution according to the gradient, take 0.1mL each and spread it on the feather meal solid medium plate, culture it upside down in a constant temperature incubator at 30°C for several days and observe it. After the colonies grow, select For well-grown, large single colonies, re-streak on LB plates, culture at a constant temperature of 30°C for 3 days, and separ...

Embodiment 2

[0056] Embodiment two: the optimization of the condition of fermented and degraded feather meal by Lactococcus

[0057] 1 Experimental method

[0058] 1.1 Medium

[0059] (1) Fermentation medium: beef extract 10g, yeast extract 10g, sucrose 100g, sodium chloride 2g, dipotassium hydrogen phosphate 10g, magnesium sulfate 0.5g, pH value 6.0, sterilized at 121°C for 20min.

[0060] (2) MRS liquid medium (seed medium): beef extract 8.0g, peptone 10.0g, yeast extract 4.0g, glucose 20.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g , magnesium sulfate 0.2g, Tween 80 1.0g, manganese sulfate 0.05g, deionized water 1000mL, pH value 5.7, sterilized at 121°C for 20min.

[0061] 1.2 Optimization of fermentation conditions

[0062] The single factor test was used to study the factors affecting the fermentation of Lactococcus feathers: the presence or absence of oxygen, time, rotation speed, temperature, inoculation amount and pH value on the...

Embodiment 3

[0102] Embodiment three: the physicochemical property analysis of fermented feather meal

[0103] 1 Experimental method

[0104] 1.1 Analysis of the Morphological Structure of Fermented Feather Meal

[0105] The feather wastes of the day were collected from the market, washed with tap water, then washed three times with distilled water, dried in an oven at 65°C to constant weight, and crushed through a 40-mesh sieve to make feather meal. Wash the fermented product of feather meal and the nylon bag together with tap water, and then wash it with distilled water three times, take out the feather meal and sterilize it in an oven at 120°C for 15 minutes, then dry it at 65°C to constant weight, crush it through a 40-mesh sieve, and The condition of embodiment two optimization prepares fermented feather meal (being albumen feed) according to the following steps, and concrete method is as follows:

[0106] (1) After the feathers are washed, they are mixed with water and agar and sti...

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Abstract

The invention discloses an application of lactococcus lactis KDLL2016-01 in preparation of a protein feed by fermenting feathers, which belongs to the technical field of animal nutrition and a feed. The application comprises the following steps: crushing feathers into feather meal, sterilizing and adding the material into a fermentation culture medium; inoculating the lactococcus lactis KDLL2016-01 into a fermentation culture medium, and carrying out fermentation culture; and after the fermentation is finished, drying a fermentation product to obtain the protein feed. According to the application, the adopted lactococcus lactis KDLL2016-01 does not generate harmful metabolites in a culture process, the product can be directly fed to livestock and poultry without detoxification and other treatment on the fermented product, and lactococcus peptide generated in the fermentation process can also kill pathogenic bacteria possibly existing in the feed, so that the quality of the feed is safer. The protein feed prepared by the method is suitable for feeding livestock and poultry.

Description

technical field [0001] The invention relates to the application of Lactococcus lactis KDLL2016-01 in fermenting feathers to prepare protein feed, and belongs to the technical field of animal nutrition and feed. Background technique [0002] At present, the lack of protein feed is a worldwide problem. Without high-quality and sufficient protein feed, there will be no high-quality and sufficient meat products. This situation has largely restricted the rapid and healthy development of the livestock and poultry industry. . my country's feather resources are very rich, but they have not been well utilized for a long time, which is mainly related to the structural properties of feathers. The feather structure is very stable, resulting in stable properties, insoluble in water, dilute acid and dilute alkali. Before processing, protease cannot hydrolyze feathers, so animals cannot digest feathers, let alone use the protein in them. The crude protein content in feathers is relatively...

Claims

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Application Information

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IPC IPC(8): A23K10/26A23K10/12A23K50/75
CPCA23K10/26A23K10/12A23K50/75A23V2400/231Y02P60/87
Inventor 徐良梅陈志辉刘洋李岚雪郭建新
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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