Bacterial Strain Tolerant to High Concentration of Chromium and Its Application
A high-concentration, strain-based technology, applied in the direction of bacteria, restoration of contaminated soil, and methods based on microorganisms, can solve problems such as secondary pollution, high cost, and difficult to deal with large-flow harmful pollution
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Embodiment 1
[0019] Isolation and Identification of Example 1 Bacterial Strain 4-1
[0020] The specific screening scheme is as follows:
[0021] Take 1g of oilfield contaminated soil (collected in Shengli Oilfield, Dongying City, China in September 2018) and add it to the screening medium, shake it in a shaker with a temperature of 30°C and a rotation speed of 160rpm for one week, and then inoculate with 5% The culture medium was transferred to fresh medium to continue the culture, and the transfer was continued for 3 times under the same conditions. Then take the culture solution for gradient dilution, pick colonies with different shapes, and use the screening medium for degradation experiments.
[0022] in:
[0023] Screening medium: add 30mg·L on the basis of LB medium -1 Cr(VI).
[0024] LB medium: peptone 10g, yeast powder 5g, NaCl 5g, distilled water 1000mL, pH 7.2-7.5.
[0025] After isolation and screening, a strain 4-1 with stable resistance to chromium was obtained, and the...
Embodiment 2
[0026] The identification of embodiment 2 bacterial strains
[0027] 1. Morphological characteristics: the colony is moist, smooth and light yellow in color.
[0028] 2. Biological and biochemical characteristics
[0030] A: Dyeing agent:
[0031] 1. Crystal violet mixed solution (A solution: crystal violet 2g, 95% ethanol 20ml; B solution: ammonium oxalate 0.8g, distilled water 80ml. Mix A and B, stand still for 48 hours and filter before use);
[0032] 2. Liquid iodine (1g of iodine, 2.0g of potassium iodide, 300mL of distilled water, first dissolve potassium iodide with 3-5mL of distilled water, then put in iodine tablets, after dissolving, add water to dilute to 300mL);
[0033] 3. Decolorization solution (95% ethanol 70mL);
[0034] 4. Counterstain solution (0.5% safranin aqueous solution).
[0035] B: Dyeing steps:
[0036] 1. Pick up a little bacterial lawn with an inoculation needle, spread it on a clean glass slide with a drop of steri...
Embodiment 3
[0072] Example 3: The enrichment ability of Stenotrophomonas acidaminiphila 4-1 to hexavalent chromium in wastewater containing hexavalent chromium
[0073] with K 2 Cr 2 o 7 Prepare Cr(VI) concentration to be 15mg L -1 , 50mg·L -1 , 100mg·L -1 , 120mg·L -1 , 150mg·L -1 , 180mg·L -1 , 200mg·L -1 , 500mg·L -1 , 1000mg·L -1 , the inoculation amount of bacterial strain 4-1 was 10% (v / v, and the bacterial density was 4.1×10 7 CFU / mL), the shaker rotates at 160 rpm and cultured at 30°C for 7 days, and the absorbance is measured at 540 nm with a visible spectrophotometer.
[0074] The specific measurement method is as follows: the culture medium is centrifuged at 4000r / min for 20min, 100μL of the supernatant is taken with a pipette gun and diluted with water to the mark in a 50mL colorimetric tube, 0.5mL of 1+1 sulfuric acid solution is added, 0.5mL of 1+ 1 phosphoric acid solution, and 2 mL of chromogen (weigh 0.2 g of diphenylcarbazide and dissolve it in 50 mL of aceto...
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