Polypeptide lyophilized powder and application thereof in preparing anti-aging cosmetics

A technology of freeze-dried powder and cosmetics, applied in the field of skin care products, can solve the problems of insufficient collagen biosynthesis and aging, and achieve good solubility, increase production, and obvious effects

Pending Publication Date: 2020-02-25
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, among the many combinations, there is no comprehensive sol

Method used

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  • Polypeptide lyophilized powder and application thereof in preparing anti-aging cosmetics
  • Polypeptide lyophilized powder and application thereof in preparing anti-aging cosmetics
  • Polypeptide lyophilized powder and application thereof in preparing anti-aging cosmetics

Examples

Experimental program
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Effect test

Example Embodiment

[0045] Example 1

[0046] An experimental model of collagen biosynthesis was established using human skin fibroblasts. The cells were treated with the freeze-dried powder of each group of polypeptides prepared in Example 1, and then the content of collagen secreted by the cells was detected by enzyme-linked immunosorbent assay to measure the effect of the additives. The experimental model of fibroblasts is as follows:

[0047] Fibroblasts are the main cellular components of loose connective tissue, with vigorous functional activities, large cells and nuclei, clear outlines, large and obvious nucleoli, weak basophilic cytoplasm, and obvious protein synthesis and secretion activities.

[0048] The fibroblast cell line was established by conventional cell culture method, and the 3rd to 6th passage cells were selected for the experimental model experiments. The culture conditions were (complete medium: DMEM (containing 2mM L-glutamine) + 10% neonatal calf serum (NCS)), temperatu...

Example Embodiment

[0055] Example 2 Preparation and efficacy verification of freeze-dried powder and matching lotion

[0056] 1. Preparation of freeze-dried powder

[0057] The freeze-dried powder and matching lotion were prepared according to the process for human efficacy experiments. The peptide combination ratio in the three groups was tripeptide-1:tripeptide-2=9:1 / 5:5 / 1:9, the percentage of the total amount of the combination 0.3%:

[0058] Table 2 Mass fraction of each component (%)

[0059]

[0060] Preparation Process

[0061] ①Glycine, trehalose, mannitol, and serum albumin were added to the container, dissolved in deionized water, and the pH was adjusted to 6.5 by sodium dihydrogen phosphate and disodium hydrogen phosphate;

[0062] ②Pre-mix the components of polysorbate-20 and polypeptide until uniform, and add them to the main container under stirring until uniform;

[0063] ③ Sterilize by UV irradiation for 30min, filter and pack with 0.22μm filter membrane;

[0064] ④ Pre-f...

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Abstract

The invention relates to the technical field of skin-care products, in particular to polypeptide lyophilized powder and application thereof in preparing anti-aging cosmetics. The polypeptide lyophilized powder provided herein comprises glycine, trehalose, mannitol, serum albumin, sodium dihydrogen phosphate, disodium hydrogen phosphate, polysorbate-20 and a polypeptide, wherein the polypeptide isselected from tripeptide-1, tripeptide-2, hexapeptide-9, hexapeptide-11, acetyl pentapeptide-1, glycyl-l-histidyl-l-lysine and carnosine. The polypeptide lyophilized powder according to the inventionhas good solubility, can increase the yield of collagen, can improve facial fine lines and brighten the skin, and therefore, has an anti-aging function.

Description

technical field [0001] The invention relates to the technical field of skin care products, in particular to freeze-dried polypeptide powder and its application in preparing anti-aging cosmetics. Background technique [0002] Skin is an important tissue and organ of human beings, and it is a powerful barrier against the invasion of external hazards. At the same time, in terms of aesthetics, delicate and smooth skin is also favored by people. However, because of complex factors such as environmental and genetic factors, especially, the superposition of environmental factors, such as ultraviolet radiation on the skin, leads to a large number of wound-like morphological changes, mainly in the dermis. Within the sun protection zone, the most pronounced changes occurred within the epidermis, mainly affecting the basal cell layer. Although its underlying mechanism is still poorly understood, increasing evidence suggests that one of the important causes is insufficient biosynthesis...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/24A61K8/34A61K8/44A61K8/49A61K8/60A61K8/9789A61Q19/08
CPCA61K8/64A61K8/44A61K8/60A61K8/345A61K8/24A61K8/4993A61K8/9789A61Q19/08A61K2800/5922A61K2800/84A61K2800/81Y02A50/30
Inventor 陈东煌岳坤罗日朗张满
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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