Preparation method of fluorine-containing polyacrylic copolymer antibacterial composite material, obtained product and application thereof

Abstract

The invention discloses a preparation method of a fluorinated polyacrylic copolymer antibacterial composite material and a obtained product and application thereof. A fluorine-free acrylic monomer andA fluorine-containing acrylate monomer are polymerized to form a fluorine-containing polyacrylic copolymer, and then the fluorine-containing polyacrylic copolymer is appropriately cross-linked to form a copolymer with a certain degree of crosslinking. Finally, the crosslinked copolymer is compounded with an antibacterial agent to form a composite antibacterial material. The preparation method ofthe invention has relatively mild reaction conditions, simple operation, and high sample yield. The obtained antibacterial composite material has the characteristics of antibacterial adhesion, sterilization, pH responsiveness, and fluorescence. The product can be made into a film or coating use on the surface of a product, not only can prevent adhesion of bacteria, dust and the like, but also canefficiently kill the bacteria attached thereon, prevent the reduction of bactericidal efficiency caused by the adhesion of dead bacteria or live bacteria. The product has good antifouling and antibacterial effects, and has good application prospects in the fields of medicine, biology, health, food and the like.

Description

technical field

[0001] The invention relates to a preparation method of a fluorine-containing polyacrylic acid copolymer antibacterial composite material, the obtained product and its application, and belongs to the technical field of polymer antibacterial materials. Background technique

[0002] Bacterial infection involves many key fields such as medical hygiene, food safety, biomedical equipment, etc., seriously threatens human health, and even causes death. It has become the largest public health problem in the world, with millions of reports reported every year. Especially in hospitals, through the contact cycle of various surfaces-medical staff-patients, infection will spread rapidly in medical institutions, and it is difficult to prevent and eradicate. It is estimated that nearly half of the nosocomial infections received by hospitalized patients are related to various surfaces. related to bacterial contamination. Previous studies have shown that once bacteria adhere...

Examples

Embodiment 1

[0051] (1) Synthesis of fluorine-containing polyacrylic acid copolymer

[0052] Monomer acrylic acid and 1H,1H,2H,2H - ethyl perfluorodecyl acrylate and azobisisobutyronitrile are dissolved with THF, the solid content of the resulting solution is 10wt%, and the solution is dissolved in N 2 Under protection, the temperature was raised to 65°C, and the temperature was kept for 8 hours. The obtained precipitate was washed three times with n-hexane, and dried for later use.

[0053] (2) Fluorine-containing polyacrylic acid copolymer cross-linked in water

[0054] Dissolve fluorine-containing polyacrylic acid copolymer in ultrapure water to prepare a solution with a concentration of 25wt%, add an aziridine crosslinking agent with a carboxyl molar mass of 5% to the solution, stir at room temperature for 1 hour, and place the solution in dialysis Dialyze the bag to remove excess crosslinker.

[0055] (3) Slightly cross-linked fluorine-containing polyacrylic acid copolymer compoun...

Embodiment 2

[0060] Prepare fluorine-containing polyacrylic acid copolymer antibacterial composite material according to the method of Example 1, the difference is: in step (1), it is prepared by RAFT polymerization, and the specific experimental steps are: acrylic acid and 1H,1H,2H,2H -perfluorodecyl ethyl acrylate, azobisisobutyronitrile and trithioester are dissolved in THF, the solid content of the solution is 10wt%, and the solution is dissolved in N 2 Under protection, the temperature was raised to 65°C, and the temperature was kept for 24 hours. The obtained precipitate was washed three times with n-hexane, and dried for later use. According to the plate test, no live bacteria were detected on the surface of the film, indicating that the film has good anti-bacterial adhesion and bactericidal effects. The antibacterial composite material has no cytotoxicity as detected by the cytotoxicity test.

Embodiment 3

[0062] Prepare fluorine-containing polyacrylic acid copolymer antibacterial composite material according to the method of Example 1, the difference is: in step (1), the used 1H,1H,2H,2H - Ethyl perfluorodecyl acrylate is replaced by perfluorooctylethyl methacrylate, the molar ratio of acrylic acid to perfluorooctylethyl methacrylate is 50:1. After the plate test, the anti-adhesion test film surface detected 10CFU / cm 2 Live bacteria, no live bacteria were detected on the surface of the film in the bactericidal experiment, indicating that the film has good anti-bacterial adhesion and bactericidal effects. The antibacterial composite material has no cytotoxicity as detected by the cytotoxicity test.

technical field

[0001] The invention relates to a preparation method of a fluorine-containing polyacrylic acid copolymer antibacterial composite material, the obtained product and its application, and belongs to the technical field of polymer antibacterial materials. Background technique

[0002] Bacterial infection involves many key fields such as medical hygiene, food safety, biomedical equipment, etc., seriously threatens human health, and even causes death. It has become the largest public health problem in the world, with millions of reports reported every year. Especially in hospitals, through the contact cycle of various surfaces-medical staff-patients, infection will spread rapidly in medical institutions, and it is difficult to prevent and eradicate. It is estimated that nearly half of the nosocomial infections received by hospitalized patients are related to various surfaces. related to bacterial contamination. Previous studies have shown that once bacteria adhere...

Examples

Embodiment 1

[0051] (1) Synthesis of fluorine-containing polyacrylic acid copolymer

[0052] Monomer acrylic acid and 1H,1H,2H,2H - ethyl perfluorodecyl acrylate and azobisisobutyronitrile are dissolved with THF, the solid content of the resulting solution is 10wt%, and the solution is dissolved in N 2 Under protection, the temperature was raised to 65°C, and the temperature was kept for 8 hours. The obtained precipitate was washed three times with n-hexane, and dried for later use.

[0053] (2) Fluorine-containing polyacrylic acid copolymer cross-linked in water

[0054] Dissolve fluorine-containing polyacrylic acid copolymer in ultrapure water to prepare a solution with a concentration of 25wt%, add an aziridine crosslinking agent with a carboxyl molar mass of 5% to the solution, stir at room temperature for 1 hour, and place the solution in dialysis Dialyze the bag to remove excess crosslinker.

[0055] (3) Slightly cross-linked fluorine-containing polyacrylic acid copolymer compoun...

Embodiment 2

[0060] Prepare fluorine-containing polyacrylic acid copolymer antibacterial composite material according to the method of Example 1, the difference is: in step (1), it is prepared by RAFT polymerization, and the specific experimental steps are: acrylic acid and 1H,1H,2H,2H -perfluorodecyl ethyl acrylate, azobisisobutyronitrile and trithioester are dissolved in THF, the solid content of the solution is 10wt%, and the solution is dissolved in N 2 Under protection, the temperature was raised to 65°C, and the temperature was kept for 24 hours. The obtained precipitate was washed three times with n-hexane, and dried for later use. According to the plate test, no live bacteria were detected on the surface of the film, indicating that the film has good anti-bacterial adhesion and bactericidal effects. The antibacterial composite material has no cytotoxicity as detected by the cytotoxicity test.

Embodiment 3

[0062] Prepare fluorine-containing polyacrylic acid copolymer antibacterial composite material according to the method of Example 1, the difference is: in step (1), the used 1H,1H,2H,2H - Ethyl perfluorodecyl acrylate is replaced by perfluorooctylethyl methacrylate, the molar ratio of acrylic acid to perfluorooctylethyl methacrylate is 50:1. After the plate test, the anti-adhesion test film surface detected 10CFU / cm 2 Live bacteria, no live bacteria were detected on the surface of the film in the bactericidal experiment, indicating that the film has good anti-bacterial adhesion and bactericidal effects. The antibacterial composite material has no cytotoxicity as detected by the cytotoxicity test.

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