Preparation method of acaudina molpadioides semper biologically active peptide

A technology of biologically active polypeptides and active polypeptides, which is applied in the field of preparation of Haitian melon bioactive peptides, can solve the problems that Haitian melon resources are not fully developed and utilized, and achieve the effects of increasing added value, promoting income and efficiency, and reducing pollution

Inactive Publication Date: 2020-03-20
WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] my country's East China Sea is very rich in resources. The current reserves are about 106 t, and the annual catch is about 3000 t. People often eat them as low-value sea cucumbers. Because sea cucumbers are hard after heating and live in shallow sea ooze, the body surface accumulates A large amount of harmful heavy metals such as lead, cadmium, and mercury make Haitian melon resources not yet fully developed and utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of preparation method of Haitian melon bioactive peptide provided by the present embodiment comprises the following steps:

[0023] (1) Beat the Haitian melon, add pure water during the beating process, the weight ratio of the purified water to the Haitian melon is 5:1, and prepare the Haitian melon slurry.

[0024] (2) Microbial domestication: Bacillus, bifidobacteria, and lactic acid bacteria were inoculated into Haitian melon slurry, and cultured for 1 day to obtain microbial slurry.

[0025] (3) Microbial fermentation and degradation: use food grade NaHCO 3 Adjust the pH of the Haitian melon slurry in step (1) to weakly alkaline 7.1; add the microbial slurry in step (2), the weight ratio of the microbial slurry to the Haitian melon slurry is 1:100; mix well, and ferment for 5 hours.

[0026] (4) Enzymolysis: continue to add compound protease, the amount of enzyme added is 4000U / g, and the enzymolysis time is 12h. After the enzymolysis is completed, the tempe...

Embodiment 2

[0031] Example 2 Determination of Polypeptide Content in Enzymolysis Supernatant

[0032] The determination of the peptide content in the enzymatic hydrolyzate was carried out with reference to the literature (Lu Wei, Ren Guopu, Song Junmei. Determination method of peptide content in protein hydrolyzate[J]. Food Science, 2005,26(7):169-171), Gly -Gly-Tyr-Arg standard products are configured into 0.0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6 and 1.8mg / ml standard solutions, respectively take 6ml of the standard solution into a 10ml centrifuge tube, add double shrinkage 2.4ml of urea reagent A and 1.6ml of biuret reagent B, mix well. Let it stand for 10 minutes, centrifuge at 2000r / min for 10 minutes, take the supernatant after centrifugation and measure the OD value under a 540nm spectrophotometer, draw the standard curve with the peptide concentration as the abscissa and the OD value as the ordinate, and the peptide content in the sample is passed The regression equation is cal...

Embodiment 3

[0038] Example 3 Distribution of polypeptides with different molecular weights in the enzymatic hydrolysis supernatant

[0039] The determination of the molecular weight of peptides usually uses the classic gel column chromatography, but this method is cumbersome and takes a long time. Using gel exclusion chromatography in HPLC and using TSK-GEL G2000SWXL as the analytical column, the molecular weight distribution of small molecular weight peptides is analyzed quickly and easily.

[0040] The molecular weight of the Haitian melon bioactive peptides prepared in Example 1 is relatively close, and the concentration is relatively high, all of which are below 3000Da. Among them, small peptides with a molecular weight of less than 1000Da are the main ones, accounting for 83.17% in total.

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Abstract

The invention discloses a preparation method of acaudina molpadioides semper biologically active peptide. The preparation method comprises the following steps of (1) mashing acaudina molpadioides semper; (2) domesticating microorganisms; (3) performing microorganism fermentation and degradation; (4) performing enzymolysis; and (5) performing centrifugation, and taking supernatant to obtain a bioactive peptide solution. The acaudina molpadioides semper is used for developing further comprehensive utilization, pollution abatement is facilitated, the added value of by-products is increased, and yield increasing and effect increasing during breeding are promoted. In the preparation technology, based on current research, the technology steps are improved, the technology time is shortened, collagen in the acaudina molpadioides semper is changed into polypeptide, the extraction rate of the polypeptide in the acaudina molpadioides semper is further increased, and the content of polypeptide being high in bioactivity and small in molecular weight in prepared polypeptide is greatly increased, and the ratio of short chain polypeptide of which the molecular weight is smaller than 1KDa is 83.17%.

Description

technical field [0001] The invention relates to a preparation method of Haitian melon bioactive peptide, which belongs to the field of biotechnology. Background technique [0002] Haitian Melon ( Acaudina molpadioides Semper ), commonly known as sea sweet potato, sea eggplant, fragrant ginseng, etc., is the taro ginseng ( Molpadida ), Jiri Ginseng ( Caudinidae ), Haitian melon ( Acaudina ) animals, the maximum body length can reach 400mm, generally 100-200mm, mainly distributed in the seabed soft mud from the tidal zone to the depth of 80 meters in the East China Sea. [0003] my country's East China Sea is very rich in resources. The current reserves are about 106 t, and the annual catch is about 3000 t. People often eat them as low-value sea cucumbers. Because sea cucumbers are hard after heating and live in shallow sea ooze, the body surface accumulates A large amount of harmful heavy metals such as lead, cadmium, and mercury make Haitian melon resources not yet ful...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P39/00C12P21/06C12P21/00C12R1/10C12R1/125C12R1/07C12R1/01
CPCC07K14/78C12P39/00C12P21/06C12P21/00
Inventor 徐仰丽苏来金杨会成胡世伟
Owner WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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