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Nano hybrid silk fibroin hydrogel and preparation method and application thereof

A technology for silk fibroin and regenerated silk fibroin, applied in the field of nano-hybrid silk fibroin hydrogel and its preparation, can solve the problem of unfavorable cell or biological factor viability, ranging from a few days to a few weeks, gel Avoiding the use of chemical cross-linking agents, good vigor, and rapid gelation without problems such as long curing time

Active Publication Date: 2020-03-27
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, traditional silk fibroin hydrogels often have the following disadvantages: 1. The gelation time is too long, generally ranging from a few days to a few weeks, which limits its clinical application; 2. Forming a gel It is necessary to add cross-linking agent or ionic salt, etc., such as the "preparation method of a silk fibroin composite gel" disclosed in patent CN103965491A, which requires the addition of cross-linking agent 1-ethyl-(3-dimethylaminopropyl) carbon Imide hydrochloride, which is not conducive to maintaining the viability of cells or biological factors embedded in the gel

Method used

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  • Nano hybrid silk fibroin hydrogel and preparation method and application thereof
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  • Nano hybrid silk fibroin hydrogel and preparation method and application thereof

Examples

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Embodiment 1

[0069] A kind of preparation method of nanoscale hydroxyapatite hybrid silk fibroin hydrogel, such as figure 2 shown, including the following steps:

[0070] (1) According to the bath ratio of 1:50 (mass ratio), add silkworm cocoons into 0.02M sodium carbonate solution and boil for 30 minutes, repeat twice, wash with pure water several times, tear loose, and dry at 40°C. Weigh the dried silk protein at a concentration of 15% by mass fraction, dissolve it at 60°C for 2 hours with a LiBr solution with a concentration of 9.3M, then put it into a dialysis bag with a molecular weight cut-off of 3500, and dialyze it with pure water at 4°C for three days. During this period, the water was changed every 6 hours, and the insoluble precipitate was removed by gauze filtration to obtain an aqueous solution of regenerated silk fibroin; it was stored in a refrigerator at 4°C.

[0071] (2) Disperse the nano-sized hydroxyapatite in the pure aqueous solution according to the concentration of...

Embodiment 2

[0078] A preparation method of nano-hybrid silk fibroin hydrogel for bone defect repair, such as figure 2 shown, including the following steps:

[0079] (1) According to the bath ratio of 1:50 (mass ratio), add silkworm cocoons into 0.02M sodium carbonate solution and boil for 30min, repeat twice, wash with pure water several times, and dry at 40°C; The 9.3M LiBr solution was dissolved at 60°C for 1 hour, so that the mass fraction of silk protein in the dissolved mixed solution was 15%, and then put into a dialysis bag with a molecular weight cut-off of 3500, and dialyzed in pure water at 10°C for three days. The polyethylene glycol solution (molecular weight is 20,000 Da) of %wt concentrates the regenerated silk fibroin aqueous solution after dialysis into the regenerated silk fibroin aqueous solution of 6%wt (determined by dry weighing method), removes insoluble precipitate by gauze filtration, Store in refrigerator at 4°C.

[0080] (2) Disperse nano-sized hydroxyapatite ...

Embodiment 3

[0085] A preparation method of nano-hybrid silk fibroin hydrogel for bone defect repair, such as figure 2 shown, including the following steps:

[0086] (1) According to the bath ratio of 1:50 (mass ratio), add silkworm cocoons into 0.02M sodium carbonate solution and boil for 30min, repeat twice, wash with pure water several times, and dry at 40°C; The 9.3M LiBr solution was dissolved at 60°C for 1 hour, so that the mass fraction of silk protein in the dissolved mixed solution was 15%, and then put into a dialysis bag with a molecular weight cut-off of 3500, and dialyzed in pure water at 10°C for three days. %wt polyethylene glycol solution (molecular weight: 20,000 Da) to concentrate the dialyzed regenerated silk fibroin aqueous solution into a 6%wt regenerated silk fibroin aqueous solution, filter through gauze to remove insoluble precipitates, and store in a refrigerator at 4°C.

[0087] (2) Disperse nano-sized hydroxyapatite in pure aqueous solution according to the con...

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Abstract

The invention discloses a nano hybrid silk fibroin hydrogel and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The nano hybrid silk fibroin hydrogel is prepared by pre-blending a regenerated silk fibroin aqueous solution and nano hydroxyapatite and performing ultrasonic treatment. According to the preparation method of the nano hybrid silkfibroin hydrogel, the ultrasonic condition and the mixing ratio of the two materials are changed, the gelation time is controlled, and rapid gelation is realized, so that use of a potentially toxic chemical crosslinking agent is avoided, and activity of cells or biological factors can be well maintained. The nano hybrid silk fibroin hydrogel provided by the invention has controllable gel performance, controllable mechanical performance, good cell compatibility and good bone induction capability, can load stem cells for repairing bone defect, and is expected to be used for embedding active factors for synergistic treatment.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to a nano-hybrid silk fibroin hydrogel and its preparation method and application. Background technique [0002] Regenerated silk fibroin is an animal-derived protein extracted from silkworm cocoons. It has the advantages of abundant resources, low immunity, adjustable biodegradability, and good blood compatibility. It has a promising application prospect in the field of bone defect repair. Natural materials. Since silkworm silk protein is different from tussah silk fibroin, it does not have arginine-glycine-aspartic acid (RGD) short peptide sequence for cell recognition, so increasing the cell responsiveness of silkworm silk biomaterials is its application. One of the main points in the field of tissue engineering. Synthetic nano-hydroxyapatite (nHA) has similar mineral phase composition and crystal structure to the inorganic components of natural bone. The preparat...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/12A61L27/22A61L27/52A61L27/50
CPCA61L27/3834A61L27/227A61L27/12A61L27/52A61L27/50A61L2430/02A61L2400/06C08L89/00
Inventor 张黎明邓立志杨凡巧王彦
Owner SUN YAT SEN UNIV
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