Nano hybrid silk fibroin hydrogel and preparation method and application thereof

A technology for silk fibroin and regenerated silk fibroin, applied in the field of nano-hybrid silk fibroin hydrogel and its preparation, can solve the problem of unfavorable cell or biological factor viability, ranging from a few days to a few weeks, gel Avoiding the use of chemical cross-linking agents, good vigor, and rapid gelation without problems such as long curing time

Active Publication Date: 2020-03-27
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, traditional silk fibroin hydrogels often have the following disadvantages: 1. The gelation time is too long, generally ranging from a few days to a few weeks, which limits its clinical application; 2. Forming a gel It is necessary to add cross-linking agent or ionic salt, etc., such as the

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  • Nano hybrid silk fibroin hydrogel and preparation method and application thereof
  • Nano hybrid silk fibroin hydrogel and preparation method and application thereof
  • Nano hybrid silk fibroin hydrogel and preparation method and application thereof

Examples

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Example Embodiment

[0068] Example 1

[0069] A preparation method of nano-level hydroxyapatite hybrid silk fibroin hydrogel, such as figure 2 As shown, including the following steps:

[0070] (1) Add the silkworm cocoons to a sodium carbonate solution with a concentration of 0.02M and boil for 30 minutes at a bath ratio of 1:50 (mass ratio), repeat twice, wash with pure water several times, tear loose, and dry at 40°C. Weigh the dried silk protein at a concentration of 15% by mass, dissolve it with a 9.3M LiBr solution at 60°C for 2 hours, then put it into a dialysis bag with a molecular weight cut-off of 3500, and dialyze it in pure water at 4°C for three days. During this period, the water was changed every 6h, and the insoluble precipitate was removed by filtration with gauze to obtain the regenerated silk fibroin aqueous solution; it was stored in a refrigerator at 4°C.

[0071] (2) Disperse nano-sized hydroxyapatite in a pure aqueous solution at a concentration of 15 mg / mL, stir for 2 hours, and...

Example Embodiment

[0077] Example 2

[0078] A preparation method of nano hybrid silk fibroin hydrogel for bone defect repair, such as figure 2 As shown, including the following steps:

[0079] (1) Add the cocoons to a 0.02M sodium carbonate solution and boil for 30 minutes at a bath ratio of 1:50 (mass ratio), repeat twice, wash with pure water several times, and dry at 40°C; use the concentration of dried silk protein The 9.3M LiBr solution was dissolved at 60°C for 1 hour to make the mass fraction of silk protein in the dissolved mixture 15%, and then put into a dialysis bag with a molecular weight cutoff of 3500, and dialyzed in pure water at 10°C for three days. %Wt polyethylene glycol solution (molecular weight: 20,000 Da) concentrates the dialyzed regenerated silk fibroin aqueous solution into a 6%wt (determined by dry weighing method) regenerated silk fibroin aqueous solution, which is filtered to remove insoluble precipitates. Store in a refrigerator at 4°C.

[0080] (2) Disperse nano-sized...

Example Embodiment

[0084] Example 3

[0085] A method for preparing nano hybrid silk fibroin hydrogel for bone defect repair, such as figure 2 As shown, including the following steps:

[0086] (1) Add the cocoons to a 0.02M sodium carbonate solution and boil for 30 minutes at a bath ratio of 1:50 (mass ratio), repeat twice, wash with pure water several times, and dry at 40°C; use the concentration of dried silk protein The 9.3M LiBr solution was dissolved at 60°C for 1 hour to make the mass fraction of silk protein in the dissolved mixture 15%, and then put into a dialysis bag with a molecular weight cutoff of 3500, and dialyzed in pure water at 10°C for three days. The %wt polyethylene glycol solution (molecular weight: 20,000 Da) concentrated the dialyzed regenerated silk fibroin aqueous solution into a 6%wt regenerated silk fibroin aqueous solution, filtered with gauze to remove insoluble precipitates, and stored in a refrigerator at 4°C.

[0087] (2) Disperse nano-sized hydroxyapatite in a pure a...

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Abstract

The invention discloses a nano hybrid silk fibroin hydrogel and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The nano hybrid silk fibroin hydrogel is prepared by pre-blending a regenerated silk fibroin aqueous solution and nano hydroxyapatite and performing ultrasonic treatment. According to the preparation method of the nano hybrid silkfibroin hydrogel, the ultrasonic condition and the mixing ratio of the two materials are changed, the gelation time is controlled, and rapid gelation is realized, so that use of a potentially toxic chemical crosslinking agent is avoided, and activity of cells or biological factors can be well maintained. The nano hybrid silk fibroin hydrogel provided by the invention has controllable gel performance, controllable mechanical performance, good cell compatibility and good bone induction capability, can load stem cells for repairing bone defect, and is expected to be used for embedding active factors for synergistic treatment.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to a nano-hybrid silk fibroin hydrogel and its preparation method and application. Background technique [0002] Regenerated silk fibroin is an animal-derived protein extracted from silkworm cocoons. It has the advantages of abundant resources, low immunity, adjustable biodegradability, and good blood compatibility. It has a promising application prospect in the field of bone defect repair. Natural materials. Since silkworm silk protein is different from tussah silk fibroin, it does not have arginine-glycine-aspartic acid (RGD) short peptide sequence for cell recognition, so increasing the cell responsiveness of silkworm silk biomaterials is its application. One of the main points in the field of tissue engineering. Synthetic nano-hydroxyapatite (nHA) has similar mineral phase composition and crystal structure to the inorganic components of natural bone. The preparat...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/12A61L27/22A61L27/52A61L27/50
CPCA61L27/3834A61L27/227A61L27/12A61L27/52A61L27/50A61L2430/02A61L2400/06C08L89/00
Inventor 张黎明邓立志杨凡巧王彦
Owner SUN YAT SEN UNIV
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