A kind of method that subcritical method prepares egg membrane protein

A subcritical, egg-film technology, applied in the preparation methods of peptides, chemical instruments and methods, pharmaceutical formulations, etc., can solve the problems of microbial contamination, low extraction efficiency, low decomposition rate and yield, etc., and can adjust the surface tension of oil and water. , reduce production cost, good effect of antioxidant activity

Active Publication Date: 2022-05-13
正大食品(襄阳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The decomposition rate and yield of this patent are low, the decomposition time is long, and the enzymatic decomposition is easy to cause microbial contamination
[0004] Chinese patent document CN103275205A discloses a method for producing water-soluble poultry egg membrane active peptides, which includes the step of hydrolyzing poultry eggshells including eggshell membranes using strong alkali (pH=12-14) and ultrasonic treatment. Although the time is short, after obtaining the hydrolyzate, it is filtered with a filter membrane with a molecular weight cut-off of 500, which allows all decomposed amino acids and oligopeptides composed of 2 to 4 amino acids to be filtered out
Among them, alkaline hydrolysis has the best effect and the shortest time, but the eggshell membrane solution after alkaline hydrolysis needs to remove alkali or salt after alkali neutralization, and the process is complicated.
There are also many problems in other methods, such as long extraction time, incomplete decomposition, low extraction efficiency, high acid-base concentration, etc., such as Chinese literature (Sodium sulfite-assisted enzymatic hydrolysis of eggshell membranes to prepare antioxidant polypeptides, Zhao Jieyuan et al., modern Food Technology) decomposes the egg membrane for 4 hours, and the molecular weight of the obtained polypeptide is 3.3-14.4kDa

Method used

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  • A kind of method that subcritical method prepares egg membrane protein
  • A kind of method that subcritical method prepares egg membrane protein
  • A kind of method that subcritical method prepares egg membrane protein

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Experimental program
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Embodiment 1

[0039] Using the ultrasonic method, the egg shells are cleaned with clear water (the mass ratio of clear water to egg shells is 4:1), the frequency of the ultrasonic wave is 10min / time, until the egg shells are free of egg whites, egg yolks and other foreign matter, and then dried to obtain clean Egg shells; then according to the method disclosed in Chinese patent document CN109276579A, using the clean egg shells to prepare eggshell membranes;

[0040] Take 5g of eggshell membrane and add it to 300mL of aqueous solution containing 0.25g of sodium hydroxide, mix well and then transfer to a subcritical reactor. The time is 1h, subcritical decomposition is carried out; after the decomposition is completed, the pH value of the obtained supernatant is adjusted to 6.8 with 0.5mol / L hydrochloric acid to obtain a decomposition solution; the decomposition solution is centrifuged at 10000r / min for 10min, and the upper The supernatant liquid was spray-dried to obtain ovalvin powder, and ...

Embodiment 2

[0043] Take 5g of eggshell membrane and add it to 300mL of aqueous solution containing 0.34g of sodium hydroxide, mix well and then transfer to a subcritical reactor. The subcritical conditions are: temperature 140°C, pressure 0.5MPa, stirring speed 350r / min , the time is 1h, carry out subcritical decomposition; after the decomposition is completed, the pH value of the supernatant obtained is adjusted to 7.0 with 0.8mol / L hydrochloric acid to obtain a decomposition solution, which is centrifuged at a speed of 10000r / min for 10min, and taken The supernatant was spray-dried to obtain ovalvin powder, and the spray-drying conditions were: outlet temperature 60°C, inlet temperature 190°C.

Embodiment 3

[0045] Take 10g of eggshell membrane and add it to 300mL of aqueous solution containing 0.8g of sodium hydroxide, mix well and then transfer to a subcritical reactor. The subcritical conditions are: temperature 130°C, pressure 2.0MPa, stirring speed 300r / min , the time is 0.5h, carry out subcritical decomposition; after the decomposition is completed, the pH value of the supernatant obtained is adjusted to 7.0 with 1mol / L hydrochloric acid to obtain a decomposition solution, which is centrifuged at 8000r / min for 8min, and taken The supernatant was spray-dried to obtain ovalvin powder, and the spray-drying conditions were: outlet temperature 70°C, inlet temperature 195°C.

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Abstract

The present invention provides a method for preparing egg-membrane by subcritical method, and relates to the technical field of egg-membrane preparation. The invention adopts the subcritical method to prepare the egg membrane, and the decomposition rate within 1 hour is as high as more than 95%; and high-priced enzymes are not needed, and the production cost is reduced. In the method of the present invention, the solution after decomposing the eggshell membrane contains free amino acids and small molecular polypeptides, so that the free amino acids and small molecular polypeptides in the prepared egg membrane protein are high in content, which satisfies the requirements of current cosmetics and health care products for low molecular weight . In the method of the present invention, in the supernatant liquid obtained by subcritical decomposition, the sodium hydroxide is directly spray-dried after neutralization, no desalting step is required, and the method has the advantages of short process, low cost, simple preparation, energy saving and the like, and the obtained eggshell Membrane polypeptide powder can be directly added to cosmetics and food after sterilization.

Description

technical field [0001] The invention relates to the technical field of egg membrane preparation, in particular to a method for preparing egg membrane by a subcritical method. Background technique [0002] At present, with the increasing production and consumption of poultry egg products, eggshells are also produced in large quantities. At present, the development and utilization of eggshells are obviously lagging behind. Most of the eggshells are discarded or buried as garbage, which will not only affect The environment is seriously polluted, and it is also an extreme waste of resources. Therefore, it is particularly necessary to carry out deep processing of eggshells. The main component of eggshell membrane is protein, with a content of more than 80%, most of which are keratin and collagen, which are similar in nature to human skin proteins. After the eggshell membrane is hydrolyzed, it can be used in cosmetics. It has the effect of eliminating skin spots such as freckles...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/465C07K1/14A61K8/98A61K8/64A61K8/20A61Q19/08A61Q5/02A61Q19/10A61Q5/12A23L33/17A23L33/175A23L33/18
CPCA61K8/982A61K8/64A61K8/20A61Q19/08A61Q5/02A61Q19/10A61Q5/12A23L33/17A23L33/175A23L33/18A61K2800/805A61K2800/48
Inventor 舒顺顺米倩刘芹胡慧花李梦婕马露牛军
Owner 正大食品(襄阳)有限公司
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