Preparation of Fluorescent Nanoparticles and Their Application in Cell Surface Sugar Detection

Abstract

The invention discloses a preparation method of glyceryl methacrylate hydrophilic brush-modified fluorescent nanoparticles and its application in cell surface sugar detection, including the synthesis of glycerol methacrylate by a reversible addition fragment chain transfer polymerization method Hydrophilic brush, and then through reversible addition fragment chain transfer precipitation polymerization method to synthesize fluorescent nanoparticles with hydrophilic polymeric brush on the surface and graft the sugar recognition element-phenylboronic acid on its surface to make cell surface sugar. Fluorescent probe; the highly hydrophilic polymer brush on the surface of the fluorescent nanoparticle has the ability to resist proteins, and the sugar recognition element-phenylboronic acid is grafted on its surface to make it have a fluorescent response to the sugar with a cis-diol structure, It has good selectivity and specificity, and can realize the specific detection of sugar in complex biological samples. The preparation process of the fluorescent nano-particles is simple and easy, and is easy to produce on a large scale.

Description

technical field [0001] The invention relates to a preparation method of fluorescent nano-particles modified by glyceryl methacrylate hydrophilic brush and its application in cell surface sugar detection. Background technique [0002] Glycosyl groups on the cell surface are involved in cell recognition and play an important role in biological signaling and tumorigenesis and metastasis. The types of glycans on the surface of different cells are also different, and the types of glycans expressed by the same cells in different growth and differentiation processes will also be quite different. Among them, the most significant glycan changes are reflected in the overexpression of sialic acid on the surface of cells when they become cancerous. However, at present, the structure of sugar molecules is complex and the types of glycosidic bonds are different, so the research on sugar is difficult, and it lags far behind proteins and nucleic acids, which are also important parts of org...

AI Technical Summary

Problems solved by technology

But at present, the structure of sugar molecules is complex and the types of glycosidic bonds are also different. Therefore, the research on sugar is very difficult. Compared with proteins and nucleic acids, which are also important components of living organisms, it is much lagging behind.

In recent years, gas chromatography, high performance liquid chromatography, liquid chromatography and enzyme-linked immunoassay have been developed successively. These methods often have the disadvantages of low sensitivity, poor specificity, complicated operation, or expensive instruments.

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