Bacillus velezensis HMB28023 and application thereof

A technology of Bacillus Velez and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of no discovery and control of Botrytis cinerea, and achieve the effect of less resistance, broad control spectrum and high control effect

Active Publication Date: 2020-05-05
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Bacillus velezensis belongs to a new species of Bacillus, and it has been used to control wheat sheath blight, wheat head blight, rape sclerotinia, pepper root rot

Method used

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  • Bacillus velezensis HMB28023 and application thereof
  • Bacillus velezensis HMB28023 and application thereof
  • Bacillus velezensis HMB28023 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The screening and isolation process of embodiment 1 HMB28023 bacterial strain

[0030] From January 16th to 20th, 2013, the Institute of Plant Protection of Hebei Academy of Agriculture and Forestry Sciences from Milin County, Gongbo Jiangda County, Nyingchi City, Tibet Autonomous Region, Gyantse County, Tingri County, Renbu County, Lazi County, Shigatse City, Kazi County, Qushui County, Lhasa City, Mozhugongka County and other 9 counties and cities collected 22 soil samples of various types such as glacier lowlands, grasslands, lakesides, canyons, and mountain passes, and weighed 1g of soil samples into 250mL containers Add 100mL of sterile water to a sterilized Erlenmeyer flask, put it on a shaker, shake at 170rpm for 30min, let it stand for 2h, take 10mL of the supernatant and add it to a 50mL sterilized centrifuge tube, put it in a constant temperature water bath at 80°C for 30 minutes, then take 1mL and Sterile water 9mL, Serve 10mL10 -3 times the soil microbial s...

Embodiment 2

[0031] The taxonomic identification of embodiment 2 HMB28023 bacterial strains:

[0032] (1) Identification of morphological characteristics

[0033]Bacteria were cultured on LB medium and were rod-shaped. After 10 hours of culture, spores were produced. The spores were mesenchymal, oval, and the cysts were not enlarged. On the nutrient agar plate, at the early stage of culture, the colonies are light milky white, pus-like, round, with neat edges, and the colonies are raised in the shape of steamed buns, with a moist surface; at the later stage of culture, the colonies are light yellow, with irregular edges, and the surface is dry and wrinkled; on the nutrient agar slant Straight line culture, in a straight line; static culture in liquid medium, the surface of the white bacterial film. These morphological characteristics are basically consistent with the morphological characteristics of Bacillus described in "Common Bacteria System Identification Manual" (edited by Dong Xiuzh...

Embodiment 3

[0046] The preparation of embodiment 3 HMB28023 microbial inoculum

[0047] Follow the steps below:

[0048] (1) Strain activation: The Bacillus velei strain HMB28023 stored at -80°C (the strain HMB28023 has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on November 25, 2019, and the preservation number is CGMCC No.19002) is activated (30°C) on LB plate medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL), pick Take a single colony on LB slant medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL) and culture at 30°C for 30 hours to obtain Activated strains;

[0049] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, water 10...

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Abstract

The present invention belongs to the field of biological prevention and control and specifically discloses a bacillus velezensis strain HMB28023. The deposit number of the bacillus velezensis strain is CGMCC No.19002. The present invention also discloses a microbial agent containing the strain. The HMB 28023 strain has a prevention effect on bortrytis cinerea of 84.39% or more, is wide in prevention and control spectrum, has the high prevention effect on the bortrytis cinerea, also has very good prevention and control effects on sphaeropsis sapinea, botryospuaeria berengeriana, valsa sordida,dothiorella gregaria and cercospora insulana, and is highly specialized for the bortrytis cinerea and not liable to produce drug resistance. A microbial inoculant is safe for humans and animals and has no environmental pollution problems.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a bacillus velei, a microbial agent produced by using the bacterium, and their application. Background technique [0002] Botrytis cinerea is a fungal disease caused by Botrytis cinerea, which can affect many parts of grapes, such as inflorescences, corollas, fruits and young stems and leaves, and the annual loss of grapes is 20%. ~30%, more than 50% in serious cases, therefore, botrytis cinerea is very harmful to grape production. [0003] The prevention and control of Botrytis cinerea mainly adopts chemical agents, cultivation measures and biological control methods. Although the control effect of chemical control is better, there are problems such as polluting the environment and easily producing drug resistance. However, the control effect of cultivation measures is poor. Biological control has been favored by more and more people because of its advantages of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/689C12Q1/04A01N63/22A01P3/00C12R1/07
CPCC12Q1/689C12N1/205C12R2001/07
Inventor 王培培王雪美李社增鹿秀云马平郭庆港张晓云曲远航苏振贺赵卫松董丽红班一云
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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