Composite magnetic nanoparticle and its preparation method and application

A technology of magnetic nanoparticles and magnetic materials, which can be used in pharmaceutical formulations, preparation of X-ray contrast agents, preparations for in vivo experiments, etc., can solve problems such as inability to achieve operation or drug delivery, cell death, and cytotoxicity, and achieve cost-effective Low, good compatibility, high safety effect

Active Publication Date: 2022-05-27
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It uses magnetic nanoparticles nGO@Fe3O4 wrapped by graphene oxide shell or magnetic nanoparticles wrapped by mesoporous silica. On the one hand, it is toxic to cells, and long-term co-culture causes cell death. On the other hand, it is affected by cell endocytosis. After (endocytosis) enters the cell, it is expelled out of the cell by exocytosis within 1 to 2 hours, making it impossible to operate or administer the cell

Method used

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  • Composite magnetic nanoparticle and its preparation method and application
  • Composite magnetic nanoparticle and its preparation method and application
  • Composite magnetic nanoparticle and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Take 10 mL of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and 50 mL of 0.12M Na 2 SO 4 solution, added to 15 mL of ZnSO 4 ·H 2 In the O solution, after stirring for 20min, 30mL of 0.27M Na was added dropwise 2 CO 3 , producing [Zn 5 (CO3) 2 (OH) 6 ] colloidal precipitation; then take 50 mL of FeSO with a concentration of 0.4 M 4 ·7H 2 Mix O and 0.2g Vc, adjust the pH of the solution to 11.0 with NaOH, then add [Zn 5 (CO 3 ) 2 (OH) 6 ] The colloidal precipitation was thoroughly mixed, and after centrifugation, it was dispersed into an ethanol solution of the same volume, and then transferred to a polytetrafluoroethylene liner in an autoclave. Standby.

[0046] Saturation magnetization test of the resulting magnetic nanoparticles (see figure 1 ) and SEM morphology characterization (see figure 2 ). The test results show that the saturation magnetization of the nanoparticles can reach 80 emu / g, the particle size is uniform, and the diameter is about...

Embodiment 2

[0049] The cytotoxicity of magnetic nanoparticles was tested by CCK8 method. Different concentrations of magnetic particles were added to a 96-well plate to inoculate rat bone marrow mesenchymal stem cells (BMSCs) and fibroblasts, respectively. Each well was inoculated with a cell suspension (100 μl / well) to make the cell volume about 1×10 6 indivual. Place the plate in an incubator for a period of time (37°C, 5% CO). 2 ). Add 10 μl of CCK solution to each well (be careful not to generate air bubbles in the wells, they will affect the OD reading). Incubate the plate in the incubator for 1-4 hours. The absorbance at 450 nm was measured with a microplate reader. The results of the toxicity of granules on bone marrow mesenchymal stem cells and fibroblasts were as follows: Figure 4 a and Figure 4 b, it shows that the magnetic nanoparticle material of this example has good biocompatibility and no cytotoxicity.

Embodiment 3

[0051] Ordinary magnetic particles (as a control) and PHBV-coated magnetic nanoparticles prepared in this example were added to a 6-well plate, inoculated with rat bone marrow mesenchymal stem cells (BMSCs), and the cell suspension (100 μl / well) was inoculated into each well. ) to make the amount of cells about 1×10 6 indivual. Incubate the plate in an incubator (37°C, 5% CO) for 12 hours. 2 ). Trypsinize, collect the suspension, use a magnet to collect the cells that have phagocytosed the magnetic particles, wash with medium every hour, and count the cells. like Figure 5 shown, the verification results show that Fe 3 O 4 @PHBV nanoparticles are better than ordinary Fe 3 O 4 Magnetic nanoparticles labeled stem cells with a longer labeling time.

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Abstract

A magnetic nano particle is composed of a magnetic material and a coating material, the magnetic material is the inner core of the magnetic nano particle, and the inner core is covered by the coating material. The nanoparticle provided by the present invention has biocompatibility, low toxicity to cells, high safety, enters into cells by endocytosis, and can be maintained in cells for more than 5 hours for a long time without being excreted out of cells by exocytosis. It is conducive to the operation of cells and the application in the medical field.

Description

technical field [0001] The invention relates to a magnetic composite material, in particular to a nanoparticle formed by coating a polymer on a magnetic particle and a preparation method thereof. Background technique [0002] Magnetic Nanoparticles / Magnetic Nanoparticles (MNPs) is a new type of material that has developed rapidly in recent years and has great application value. Environmental protection has been more and more widely used. [0003] Magnetic composite nanoparticles are nano-scale particles, generally composed of a magnetic core composed of metal oxides such as iron, cobalt, and nickel, and a polymer / silicon / hydroxyapatite shell layer wrapped around the magnetic core. The most common core layer consists of Fe with superparamagnetic or ferromagnetic properties 3 O 4 or γ-Fe 2 O 3 It has magnetic guidance (targeting), and can realize directional movement under the action of an external magnetic field, which is convenient for positioning and separation from th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/12A61K49/04A61K49/00A61K9/51A61K47/34A61K47/02A61K47/59A61K47/69C12N5/0775
CPCA61K49/1857A61K49/126A61K49/049A61K49/0428A61K49/0093A61K49/0054A61K9/5153A61K9/5115A61K47/34A61K47/02A61K47/593A61K47/6935A61K47/6929C12N5/0663
Inventor 王贤松
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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