Check patentability & draft patents in minutes with Patsnap Eureka AI!

Inorganic-organic hybrid keggin-type polyoxometalate complex, its preparation method and its application in antibacterial

A technology of polyoxometalates and organic hybridization, which is applied in organic chemistry, antibacterial drugs, organic active ingredients, etc., can solve the problems of instability of pure inorganic substances, limit cell penetration, etc., and achieve good bactericidal effect Effect

Active Publication Date: 2022-07-22
HENAN UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure inorganics exhibit instability under physiological conditions and limit cell penetration due to their surface properties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Inorganic-organic hybrid keggin-type polyoxometalate complex, its preparation method and its application in antibacterial
  • Inorganic-organic hybrid keggin-type polyoxometalate complex, its preparation method and its application in antibacterial
  • Inorganic-organic hybrid keggin-type polyoxometalate complex, its preparation method and its application in antibacterial

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method of an inorganic-organic hybrid Keggin-type polyoxometalate complex, comprising the following steps:

[0036] The Co(NO 3 ) 2 ·6H 2 O (0.189 g, 1.3 mmol), Na 2 MoO 4 ·2H 2 O (0.48 g, 2.0 mmol), EDTMP (0.35 g, 0.8 mmol), and 2-acetylpyrazine thiosemicarbazide (0.097 g, 0.5 mmol) were added to 60 mL of water and methanol (2:1 by volume) In the mixed solvent, stir for 40 minutes until the dissolution is complete, adjust the pH value between 2.5 and 3.0 with dilute hydrochloric acid, then transfer it to a reaction flask, put it in a polytetrafluoroethylene liner, keep it at 130 ° C for 3 days, and cool it. At room temperature, washed with water and air-dried to obtain black quadrilateral crystals, which are composites.

[0037] The above-mentioned inorganic-organic hybrid Keggin-type polyoxometalate belongs to the monoclinic crystal system, C2 / c space group, whose unit cell parameters are: a = 29.1829(13) Å, b = 16.5031(7) Å, c = 18.9095(9) Å...

Embodiment 2

[0047] Antibacterial effect evaluation method 1 Disc diffusion method:

[0048] Taking DMSO as blank control group, the antibacterial activity of this material was studied by agar plate test. The complexes were diluted with DMSO in gradients (1 mg / mL, 0.5 mg / mL, 0.25 mg / mL, 0.125 mg / mL, 0.0625 mg / mL, 0.03125 mg / mL…) prior to testing. All materials used during testing were autoclaved and nutrient agar plates (NA) were sterilized by UV radiation for 1 hour. will 10 7 CFU / mL of Staphylococcus aureus ( S. aureus ), Bacillus subtilis ( B. subtilis ) and 10 6 CFU / mL of Escherichia coli ( E. coli ) and Agrobacterium tumefaciens ( A. tumefaciens) were evenly coated on the nutrient agar (NA) plate, and then punched, and the prepared complexes were sucked into the agar plate holes in turn, then sealed, and the culture was incubated at 37 ° C for 16 hours. , and finally, the size of the zone of inhibition (ZOI) of all samples was measured, and the optical images were recorded...

Embodiment 3

[0054] Time Kill Experiment Evaluation 2 Colony Forming Unit (CFU) Method:

[0055] The bactericidal ability of the complex was studied by plate coating culture method. Serial dilutions of E. coli and S. aureus solutions to 10 5 CFU / mL, and prepare a 1 mg / mL complex solution. Equal volumes of inoculated bacterial solution and complex solution were incubated at 37°C for different times. The bacterial solution without complexes was used as a control experiment. The mixed solution incubated at different times was evenly spread on nutrient agar (NA) plates. The assay was repeated three times and incubated in a constant temperature incubator at 37°C for 16 hours. The experiment continued until few colonies were observed. The experimental results show that Figure 9 and 10 . Bacterial cell survival rate (%) = (the number of colonies in the experimental group / the number of colonies in the control group) × 100%.

[0056] Figure 9 and Figure 10 The complex pairs of the pres...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention discloses an inorganic-organic hybrid Keggin-type polyoxometalate complex, the chemical formula of which is: [Co(L) 2 ] 3 [PMo 12 O 40 ], in the formula HL=2-acetylpyrazine thiosemicarbazone; the compound belongs to the monoclinic system, C2 / c space group, the unit cell parameters are: a =29.1829(13)Å, b =16.5031(7)Å, c =18.9095(9)Å, α =90°, β =103.465(1), γ =90°. The complex self-assembles 2-acetylpyrazine thiosemicarbazone with POM, which not only enhances the stability and penetration ability of POM, but also makes it have excellent antibacterial properties. It can play a considerable antibacterial effect in industry and other application fields, such as being used as antibacterial materials, antibacterial reagents or preparing antibacterial drugs.

Description

technical field [0001] The invention belongs to the technical field of inorganic-organic functionalized polyacids. In particular, it relates to a combination of 2-acetylpyrazine thiosemicarbazide compound with good bactericidal effect and POM with high redox, anticancer and antimicrobial activities to prepare inorganic-organic hybrid Keggin-type polyoxometalates salt complex. The complex self-assembles 2-acetylpyrazine thiosemicarbazone with POM, which not only enhances the stability and penetration ability of POM, but also makes it have excellent antibacterial properties, which can be used in biological and food industries and other applications The field plays a considerable antibacterial effect. Background technique [0002] Bacterial infections face serious problems that can threaten human health and the growth of animals and plants. Overuse and even abuse of antibiotics can make bacteria resistant to antibiotics. Today, millions of people around the world die every ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D241/12A61K31/4965A61P31/04A61K33/24A61K47/55
CPCC07D241/12A61P31/04A61K33/24A61K47/55
Inventor 李明雪赵萌刘泰宇方颜刘静
Owner HENAN UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com