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Multifunctional pediococcus pentosaceus CCFM1107 for relieving toxic actions of PFOS (perfluorooctane sulfonate) and fermentation food and application of CCFM1107

A technology of CCFM1107 and Pediococcus pentosaceae, which is applied in the field of microorganisms, can solve the problems of high price and difficulty in obtaining, and achieve the effects of reducing content, improving liver enlargement, and improving intestinal flora disorder

Active Publication Date: 2020-05-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these natural substances are expensive and difficult to obtain. In addition, due to the tolerance of the human body, it is not yet known whether a large amount of intake will cause potential harm to the human body.

Method used

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  • Multifunctional pediococcus pentosaceus CCFM1107 for relieving toxic actions of PFOS (perfluorooctane sulfonate) and fermentation food and application of CCFM1107
  • Multifunctional pediococcus pentosaceus CCFM1107 for relieving toxic actions of PFOS (perfluorooctane sulfonate) and fermentation food and application of CCFM1107
  • Multifunctional pediococcus pentosaceus CCFM1107 for relieving toxic actions of PFOS (perfluorooctane sulfonate) and fermentation food and application of CCFM1107

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Pediococcus pentosaceae CCFM1107 has good PFOS adsorption capacity

[0067] Bacterial adsorption Pediococcus pentosaceae CCFM1107 was purified and activated for culture, inoculated in MRS liquid medium at 1% (v / v) inoculum, and cultured at 37°C for 12 hours. Then centrifuge at 8000r / min for 5min to collect the bacteria, take the precipitate and clean it with normal saline, and continue to centrifuge at 8000r / min for 5min to remove the precipitate to obtain live bacteria cells, that is, wet bacteria. The wet bacteria were resuspended in 20mg / L PFOS solution, and the final bacterial cell concentration reached 1g dry cells / L (wet bacteria were resuspended in PFOS-free ultrapure water as a blank control). Use 0.1M NaOH or HCl solution to quickly adjust the pH of the PFOS solution containing bacteria to 3.0, add a small amount of NaOH or HCl (less than 0.5ml), and its ionic strength has negligible effect on PFOS adsorption. Subsequently, the 250ml Erlenmeyer flas...

Embodiment 2

[0072] Example 2: Pediococcus pentosaceae CCFM1107 has a good ability to scavenge diphenyltrinitrophenylhydrazine free radicals (DPPH) in vitro, scavenge hydroxyl free radicals and reducing ability;

[0073] After fully mixing 1 mL of Pediococcus pentosaceae CCFM1107 complete cell suspension with 1 mL of freshly prepared DPPH absolute ethanol solution (0.2 mmol / L), react in the dark at 37°C for 30 min. Mix DPPH and PBS (pH7.2) as a control sample, and culture under the same conditions. After centrifuging at 7000×g for 10 min, measure the absorbance at 517 nm, and calculate the ability of lactic acid bacteria to scavenge DPPH free radicals according to the following formula:

[0074] DPPH radical scavenging rate (%)=[1-A517 (sample) / A517 (control)]×100%.

[0075] Mix 1 mL of 1,10-phenanthroline, 1 mL of LPBS (pH 7.2), 1 mL of Pediococcus pentosaceae CCFM1107 complete cell suspension or 1 mL of FeSO4 (referred to as "mixture 1"). Add 1 mL of H to "Mixture 1" 2 o 2 , in a wat...

Embodiment 3

[0078] Example 3: Pediococcus pentosaceae CCFM1107 can significantly improve liver enlargement in mice exposed to PFOS

[0079] Twenty-four 6-week-old male C57BL / 6J mice were randomly divided into four groups according to body weight after acclimating to the environment for one week: blank control group, model group, quercetin intervention group, and Pediococcus pentosaceae CCFM1107 intervention group. Each group contained 6 See Table 2 for animal grouping and treatment methods.

[0080] Table 2 Grouping and processing methods of animal experiments

[0081]

[0082]

[0083] The mice in Example 2 were weighed on the 31st day, then euthanized, blood was taken from the orbit, the liver was taken out and weighed wet to calculate the organ coefficient, and the organ coefficient of the mouse liver was calculated according to the following formula:

[0084] Liver organ coefficient = liver wet weight (g) / mouse body weight before euthanasia (100g)

[0085] Experimental results...

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Abstract

The invention discloses multifunctional pediococcus pentosaceus CCFM1107 for relieving toxic actions of PFOS (perfluorooctane sulfonate) and a fermentation food and application of CCFM1107. The pediococcus pentosaceus CCFM1107 disclosed by the invention has a very good adsorption capability upon PFOS in vitro to reduce the concentration of PFOS, has a good capability in eliminating diphenyl trinitrophenyl hydrazyl free radicals and hydroxyl free radicals, and a reduction capability, and is capable of relieving hepatomegaly caused by exposure of PFOS to mice, reducing the content of a TNF-alpha(tumor necrosis factor-alpha) in the liver, relieving intestinal flora disturbance, reducing the abundance of S24-7 families, lactobacillus, bifidobacterium and parabacteroides in intestines of the mice, and reducing the occurrence tendency of diseases such as liver diseases, hypertension, diabetes and obesity.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to multifunctional Pediococcus pentosaceae CCFM1107 for alleviating the toxic effect of PFOS, fermented food and applications thereof. Background technique [0002] Perfluorinated compounds have been widely used in various industries due to their hydrophobic and oleophobic properties, as well as good thermal stability, chemical stability and biological stability. Used in ready-to-wear (such as waterproof, stain-resistant outdoor clothing) and home textiles (such as carpets, furniture fabrics, etc.), takeaway food containers, personal care products (such as dental floss), and fire fighting foam. Among them, PFOS, as one of the final transformation products of various fluorine compounds, can directly cause harm to the human body through environmental exposure, or enter the body of animals and plants and bioaccumulate in the human body through the food chain, so it has bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/741A61P39/02A61P1/16A61P9/12A61P3/10A61P3/04A23C9/123A23C19/00A23C13/00A23L19/20A23L33/00A23C20/02C12R1/01A23L11/50
CPCA61K35/741A61P39/02A61P1/16A61P9/12A61P3/10A61P3/04A23C9/123A23C19/00A23C13/00A23L19/20A23L33/00A23C20/025A23V2002/00C12R2001/01C12N1/205A23V2400/427A23V2200/334A23V2200/30
Inventor 赵建新王刚孙姗姗朱慧越张灏陈卫
Owner JIANGNAN UNIV
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