A fruit tree seedling root fungicide compounded by tolclofos-methyl and pyraclostrobin and its preparation method, use method and application
A technology of tolclofos-methyl and pyraclostrobin, which is applied in the direction of fungicides, botany equipment and methods, applications, etc., can solve the problems of rarely used, large dosage, poor preventive effect, etc. Large-scale production, low production cost, and long-lasting effect
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Embodiment 1
[0122] Example 1: Proportion experiment of pyraclostrobin and tolclofos-methyl composition
[0123] In this experiment, the white silkworm (collected from Yiyuan, Shandong, the same below) was used as the test strain. The isolated and purified bacterial strains were cultivated in potato dextrose agar (PDA) medium at 25°C for 3 days, when the colony radius reached 2 / 3 of the medium radius, they were punched into bacterial cakes with a 0.5cm hole puncher for later use.
[0124] The inhibitory effect of pyraclostrobin and tolclofos-methyl on the mycelial growth of S. albicans was determined by mycelial growth rate method. Pyraclostrobin and tolclofos-methyl were prepared into 0.1 μg / mL (ppm) and 1.5 μg / mL (ppm) liquid medicines respectively, and the ratio by volume was 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 3:7, 2:8, 9:1, 10:0 mixed, a total of 11 chemical treatments, with water as the blank control. Mix the medicinal liquid into the PDA medium, inoculate the fungus cake of S. can...
Embodiment 2
[0129] Example 2: Toxicity determination of the ratio combination of pyraclostrobin and tolclofos-methyl
[0130] When the mass ratio of pyraclostrobin and tolclofos-methyl was 1:22.5, the mycelium growth rate method was used to determine its inhibitory effect on the mycelial growth of Sepia spp. The drug concentrations were 11.7, 5.9, 2.9, 1.5, 0.7, 0.4, 0.2 μg / mL (ppm), and the drug solution was mixed into the PDA, and the solvent was used as a blank control. Inoculate the fungus cakes of Pseudomonas spp. and P. spp. in the center of the PDA plate containing the medicament, and culture at 25°C. When the growth diameter of the hyphae in the control treatment reaches 2 / 3 of the diameter of the petri dish, measure the colony diameter with the cross method , calculate the inhibitory rate of the agent on the mycelial growth of the pathogen, fit the virulence regression equation, and calculate the EC50, correlation coefficient, and compound co-toxicity coefficient.
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Embodiment 3
[0140] According to the formula in Table 3 below, configure the primary preparations of 9 experimental groups, and detect the suspension rate, viscosity, pouring property, centrifugal stability, film-forming effect and water dispersibility of the primary preparations of each experimental group, and compare the experimental groups The comprehensive performance of the primary preparation, the test results are shown in Table 4.
[0141] Table 3 Experimental group setting scheme
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[0144] Table 4 Test results of different experimental groups
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[0146] From the above results, it can be seen that the overall performance of the 9 experimental groups is good, among which, the overall performance of the experimental group 8 is the best, not only has the highest suspension rate, but also has moderate viscosity, short film-forming time and excellent film-forming effect.
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