Application of tremella fuciformis spores or fermented products thereof as ultraviolet isolating ingredient of cosmetic
A technology of tremella spores and fermented products, which is applied in the field of cosmetics, can solve the problems of few researches on tremella spores, and achieve the effect of meeting the needs of skin care and sun protection, with small stimulation and high yield
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Embodiment 1
[0022] Embodiment 1. The preparation of white fungus spore fermentation product
[0023] (1) Preparation of medium
[0024] Seed medium (wt%): peptone 0.5%; magnesium sulfate 0.15%; dipotassium hydrogen phosphate 0.3%; glucose 2%; vitamin B10.002%, the balance of water. Mix the ingredients according to the above proportions, adjust the pH to 5.0, and sterilize at 116°C for 20 minutes.
[0025] Fermentation medium (wt%): 0.2% peptone; 0.3% ammonium sulfate; 0.15% magnesium sulfate; 0.3% dipotassium hydrogen phosphate; 3% glucose; Mix the ingredients according to the above proportions, adjust the pH to 6.0, and sterilize at 116°C for 20 minutes.
[0026] (2) fermentation
[0027] The spores of Tremella fungus were inoculated into 300 mL seed medium, and cultured at 25°C and 200 rpm for 3 days to obtain seed liquid. The seed liquid was inoculated into a 5 L fermenter at 8% (volume percentage, the same below), and the liquid volume of the fermenter was 3.5 L. The rotation spe...
Embodiment 2
[0030] Embodiment 2. raw material safety evaluation
[0031] In order to verify the safety of the tremella spore fermented product prepared in Example 1 as a cosmetic raw material, 30 volunteers were recruited to conduct a skin patch test. The experimenters were 22-35 years old, 10 males and 20 females. The tremella spore fermented product was prepared into a 10wt% aqueous solution, applied quantitatively on a patch tester, pasted on the inner side of the upper arm, and observed whether the skin had an inflammatory reaction after 24 hours.
[0032] The results showed that 2 of the 30 volunteers experienced slight redness, swelling and itching, and the rest showed negative reactions, indicating that the raw material has good safety and can be added to cosmetics.
Embodiment 3
[0033] Example 3. Verification of the effectiveness of raw materials in blocking and isolating ultraviolet rays
[0034](1) Experimental samples
[0035] 5% Tremella spore sugar solution: Weigh 5 g of Tremella spore fermentation product powder prepared in Example 1, dissolve in 100 mL of water, and stir evenly.
[0036] 5% titanium dioxide solution: weigh 5g titanium dioxide powder, dissolve in 100 mL dioctyl carbonate solution, and stir evenly.
[0037] 5% zinc oxide solution: weigh 5g of zinc oxide powder, dissolve in 100 mL of dioctyl carbonate solution, and stir evenly.
[0038] 4% 4-methylbenzylidene camphor solution: Weigh 4 g of 4-methylbenzylidene camphor (purchased from Shanghai Qiuyan Trading Co., Ltd.), dissolve it in 100 mL of dioctyl carbonate solution, and stir well.
[0039] 4% butylmethoxydibenzoylmethane solution: Weigh 4g of butylmethoxydibenzoylmethane (purchased from Shanghai Qiuyan Trading Co., Ltd.), dissolve it in 100 mL of dioctyl carbonate solution, ...
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