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Method for determining acrylamide in edible oil

A technology of acrylamide and determination method, applied in chemical instruments and methods, measuring devices, material separation and other directions, can solve problems such as unreported detection of acrylamide, and achieve the effects of high recovery rate, small matrix effect, and improved detection efficiency

Active Publication Date: 2020-06-09
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, GPC has been used for oil separation of some oily samples, but it has not been reported for the detection of acrylamide in edible vegetable oil.

Method used

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  • Method for determining acrylamide in edible oil
  • Method for determining acrylamide in edible oil
  • Method for determining acrylamide in edible oil

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Embodiment 1

[0045] 1 Instruments and materials

[0046] TSQ Quantiva liquid chromatography-triple quadrupole tandem mass spectrometer, equipped with electrospray ion source, ThermoFisher, USA; Autoclean gel permeation chromatography, LabTech, USA; Sigma 3-18K high-speed refrigerated centrifuge, Sigma, Germany; ultrasonic Cleaner, Ningbo Xinzhi Biotechnology Co., Ltd.; MS3 Vortex Mixer, IKA Company, N-EVAP-45 Nitrogen Blowing Apparatus, American Organomation Company; SQP-electronic balance, Sartorius Scientific Instrument Co., Ltd.; ultrapure water , Mili-Q ultrapure water machine.

[0047] Acrylamide standard substance (purity ≥ 99%), German Dr company, 13 C 3 - Acrylamide isotope internal standard (purity ≥ 99%), Germany WITEGA Company; Methanol, acetonitrile, cyclohexane, ethyl acetate (chromatographically pure), U.S. Bruker Company; Formic acid, ammonium acetate (chromatographically pure), U.S. Sigma-Aldrich Company; water is prepared by Milli-Q ultrapure water machine; nitrogen (>9...

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Abstract

The invention discloses a method for determining acrylamide in edible oil. The method comprises the following steps: (1) performing pretreatment; (2) preparing a standard solution; (3) obtaining a liquid chromatogram and a regression equation; (4) performing qualitative analysis; (5) performing quantitative calculation, and quantifying by adopting a stable isotope internal standard method: calculating the concentration of acrylamide by adopting the regression equation in the step (3) according to the areas of chromatographic peaks of an acrylamide quantitative ion pair and a 13C3 acrylamide internal standard quantitative ion pair in a sample. The pretreatment operation is simple and efficient, the automation degree is high, the gel chromatography online purification is achieved, the problem that acrylamide is prone to loss in pretreatment is solved, and the method is good in recovery rate, high in sensitivity and accurate in qualitative and quantitative analysis.

Description

technical field [0001] The invention relates to a method for determining acrylamide in edible oil, belonging to the technical field of food detection. Background technique [0002] Acrylamide is a nitrogen-containing polar organic small molecule, a monomer of organic synthetic materials, and an intermediate in the production of medicines, dyes, and paints. It was listed as a Class 2A carcinogen by the International Agency for Research on Cancer (IARC) in 1994. It has various toxic and side effects such as neurotoxicity, reproductive toxicity, mutagenicity and carcinogenicity to animals. Early attention to acrylamide was mainly focused on occupational exposure. In 2002, Swedish scientists reported for the first time that acrylamide was found in baked, fried and other foods, and the content of acrylamide in French fries was as high as 3.9mg / kg. Since then, the pollution of acrylamide in food has attracted people's attention. The United States, the European Union, Canada, my c...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72B01J20/281
CPCG01N30/48G01N30/02G01N30/06G01N30/34G01N30/36G01N30/7266G01N2030/027G01N2030/045B01J20/291
Inventor 张艳侠刘艳明徐向军孙珊珊赵慧男祝建华李晓宿书芳薛霞郑文静
Owner SHANDONG INST FOR FOOD & DRUG CONTROL