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Method for extracting soybean protein from soybean meal

A technology for soybean protein and soybean meal, applied in the field of protein extraction, can solve the problems of difficulty in protein extraction and low utilization rate of soybean meal, and achieve the effects of improving the recovery rate, easily enlarging the production process, and reducing the steps of pH value measurement and adjustment.

Inactive Publication Date: 2020-06-12
DALIAN UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to propose a method for extracting soybean protein from soybean meal, aiming at the low utilization rate of soybean meal and difficult protein extraction. The method has the advantages of high protein extraction rate, good antioxidant activity and easy operation. The characteristics of strong repeatability, safe and pollution-free process provide an effective way for the green and efficient production of soybean protein

Method used

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Examples

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Embodiment 1

[0024] This embodiment discloses a method for extracting soybean protein from soybean meal, which is characterized in that it comprises the following steps:

[0025] Step (1) selecting soybean meal after oil extraction, grinding and pulverizing through a (10 mesh) sieve by a pulverizer to obtain soybean meal powder with a particle size not greater than 2 mm;

[0026] Step (2) Add 0.02g NaOH per gram of soybean meal, mix well, then add 0.005g vitamin C, mix well, then add 10mL pure water, stir well, extract at 50°C at a constant temperature, and keep stirring or Shock 2h;

[0027] Step (3) centrifuging the extract to obtain the extract supernatant and extract residue;

[0028] Step (4) Separate the leaching supernatant of step (3), adjust the pH value to 4.0 with (1 mol / L of) hydrochloric acid, add 0.005 g of pregelatinized starch, mix well, and let it stand for precipitation at room temperature for 0.5 h. Subsequent centrifugation to obtain protein precipitate I;

[0029] S...

Embodiment 2

[0034] This embodiment discloses a method for extracting soybean protein from soybean meal, which is characterized in that it comprises the following steps:

[0035] Step (1) selecting soybean meal after oil extraction, grinding and pulverizing through a (10 mesh) sieve by a pulverizer to obtain soybean meal powder with a particle size not greater than 2mm;

[0036] Step (2) Add 0.03g NaOH per gram of soybean meal, mix well, then add 0.01g vitamin C, mix well, then add 10mL pure water, stir well, extract at a constant temperature at 60°C, and keep stirring or Shock 3h;

[0037] Step (3) centrifuging the extract to obtain the extract supernatant and extract residue;

[0038] Step (4) Separate the leaching supernatant of step (3), use (1mol / L of) hydrochloric acid to adjust the pH value to 4.3, add 0.01g of pregelatinized starch, mix well, let stand at room temperature for 1h, and then Centrifuge to obtain protein precipitate I;

[0039] Step (5) separate the extract residue of...

Embodiment 3

[0044] This embodiment discloses a method for extracting soybean protein from soybean meal, which is characterized in that it comprises the following steps:

[0045] Step (1) selecting soybean meal after oil extraction, grinding and pulverizing through a (10 mesh) sieve by a pulverizer to obtain soybean meal powder with a particle size not greater than 2mm;

[0046] Step (2) Add 0.04g NaOH per gram of soybean meal, mix well, then add 0.02g vitamin C, mix well, then add 10mL pure water, stir well, extract at a constant temperature at 70°C, and keep stirring or Shock 6h;

[0047] Step (3) centrifuging the extract to obtain the extract supernatant and extract residue;

[0048] Step (4) Separate the leaching supernatant of step (3), use (1mol / L of) hydrochloric acid to adjust the pH value to 4.5, add 0.02 g of pregelatinized starch, mix well, let stand at room temperature for 2 hours, and then Centrifuge to obtain protein precipitate I;

[0049] Step (5) separate the residue of...

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Abstract

The invention provides a method for extracting soybean protein from soybean meal. The method comprises the following steps: grinding, crushing and sieving the soybean meal to obtain soybean meal powder; adding NaOH, vitamin C and purified water into the soybean meal powder, and carrying out primary extraction under high-temperature oscillation; carrying out centrifugation to obtain a supernatant,carrying out pH value adjustment on the supernatant and adding a co-precipitation agent to obtain a soybean protein primary precipitate, carrying out secondary extraction on the centrifugation residue, and carrying out pH value adjustment and adding the co-precipitation agent to obtain a secondary precipitate; and combining the two precipitates, and carrying out freeze-drying treatment to obtain the soybean meal protein powder. According to the invention, high-purity soybean meal protein powder is obtained; by optimizing and limiting the additon amount of NaOH and the extraction temperature, the extraction efficiency of the soybean protein is improved, the repeatability of the extraction process is improved, the antioxidant activity of the protein is protected by virtue of vitamin C, the browning process is avoided, and the efficient and stable soybean protein extraction method is established.

Description

technical field [0001] The invention relates to protein extraction technology, and specifically provides a method for extracting soybean protein from soybean meal. Background technique [0002] Soybean meal is a by-product obtained after extracting soybean oil from soybeans. Its protein content is 40-50%, which is 4-5 times that of cereals, almost twice that of meat, eggs, and fish, and 1 ton of soybeans are used in production. Soybean oil can get 0.8 tons of soybean meal. Soybean protein in soybean meal is a kind of plant-based protein, and its amino acid composition is similar to that of milk protein. Except for slightly lower methionine, other essential amino acids are rich in content, and its nutritional value is similar to that of animal protein. Foods made of soybean meal protein have no adverse side effects and are rich in nutrition. Soybean meal protein drinks are easier to digest and absorb than milk, can remove excess sterol metabolites in the blood, and have obvi...

Claims

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Application Information

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IPC IPC(8): A23J1/00A23J1/14
CPCA23J1/005A23J1/14A23V2002/00A23V2200/048A23V2250/5488A23V2300/14A23V2300/40A23V2300/38
Inventor 徐卫平任苗苗陈继红陈金龙王大超许建强
Owner DALIAN UNIV OF TECH
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