Low-grade fine-grained tin ore biological collecting agent and preparation method and application thereof

A collector, low-grade technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of environmental pollution, large consumption, and low recovery rate of flotation fine particles.

Pending Publication Date: 2020-07-07
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, most of the existing collectors are chemical beneficiation agents, which pollute the environment, and the recovery rate of the flotation fine particle ore is not high and the consumption is large

Method used

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  • Low-grade fine-grained tin ore biological collecting agent and preparation method and application thereof
  • Low-grade fine-grained tin ore biological collecting agent and preparation method and application thereof
  • Low-grade fine-grained tin ore biological collecting agent and preparation method and application thereof

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preparation example Construction

[0042] In the present invention, the source of the Rhodococcus opacus (R. opacus) is not particularly limited, and a conventional source of Rhodococcus opacus well known to those skilled in the art can be used. In the present invention, the concentration of the Rhodococcus opacus in the biological collector is preferably 0.1-0.4 g / L, more preferably 0.3 g / L. The above concentration can ensure that the absorption of cassiterite and quartz sand is relatively smaller when the strains have relatively greater absorption of cassiterite. In the present invention, the preparation method of Rhodococcus turbidity comprises the following steps: inoculate Rhodococcus turbidity into liquid culture medium, culture at 20-40°C, 150-200r / min for 24-96h, 1000-3000r / min Centrifuge for 6-10 minutes, take the supernatant, centrifuge at 8000-12000 r / min for 10-30 minutes, take the precipitate, and obtain the biological collector. In the present invention, described cultivation is more preferably 3...

Embodiment 1

[0056] Culture of Rhodococcus opacus (R.opacus)

[0057] 1. R.opacus medium formula: glucose 10-60g / L, peptone 1-15g / L, yeast extract 2-20g / L, pH value 3.5-6.5.

[0058] 2. Put the medium into the Erlenmeyer flask, wrap it up and put it in an autoclave to sterilize for 25-65 minutes. After cooling down to room temperature, inoculate R.opacus into the medium. The cultivation temperature is respectively 20-40° C., and the rotation speed is 150-200 r / min, and the corresponding cultivation time is 24-96 hours. Centrifuge the cultured bacteria solution at a centrifuge speed of 1000-3000r / min for 6-10min to remove the precipitated substances in the culture solution, and then centrifuge at 8000-12000r / min for 10-30min to obtain wet bacteria mud. Disperse the bacteria sludge with distilled water, then centrifuge under the same conditions, repeat three times, weigh and record to obtain the wet weight of the bacteria, add 1.0-6.5% NaCl to make different concentrations of bacteria susp...

Embodiment 2

[0103] R.opacus has a very good selective adsorption effect on pure cassiterite and quartz sand. When R.opacus is used as a reagent to study the influence of pH on the adsorption rate, change the pH to determine its effect on the adsorption rate of pure quartz sand and refined tin ore. influence, the adsorption rate results of R.opacus on quartz sand and refined tin ore at different pH values ​​are as follows Figure 5 .

[0104] from Figure 5 It can be seen that the influence of pH on the adsorption rate is obvious, and as the pH increases, the adsorption of R. The adsorption rate gradually decreases with the increase of pH. When the pH=2, the adsorption rate is 30%, and when the pH=11, the adsorption rate is almost 0. The higher adsorption rate is due to the fact that R. Caused by cell aggregation under acidic conditions.

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Abstract

The invention relates to a low-grade fine-grained tin ore biological collecting agent and a preparation method and application thereof, which belong to the technical field of cassiterite flotation. The low-grade fine-grained tin ore biological collecting agent disclosed by the invention comprises rhodococcus turbid. In an ore flotation system, specific adsorption exists between a microorganism collecting agent and ore, the microorganism collecting agent is developed, the use of a chemical collecting agent can be reduced, and the defects that in the flotation process of using the chemical agent, the agent use amount is large, the agent recovery rate is low, and severe damage to the environment is likely to be caused are overcome.

Description

technical field [0001] The invention relates to the technical field of cassiterite flotation, in particular to a low-grade fine-grained tin ore biological collector and its preparation method and application. Background technique [0002] Low-grade fine-grained fine ore contains minerals such as pyrite, arsenopyrite, limonite, chalcopyrite, brittle pyrite, iron propylene zinc ore, and impurities such as quartzite and calcite. Calcite and quartz sand need to be removed. At present, the types of cassiterite flotation collectors are: [0003] Fatty acids: palmitic acid, stearic acid, oleic acid, linoleic acid, ricinoleic acid, etc. [0004] Alkyl sulfosuccinamic acid salts: cetyl sodium sulfate, sulfonic acid groups, alkyl sulfosuccinic acid salts, tetrasodium sulfosuccinic acid, alkyl dicarboxylic acids, etc. [0005] Arsenic acid and phosphoric acid: toluene acid, benzyl acid, styrene phosphonic acid, alkylamine dimethyl phosphonic acid, alkyl phosphite, dialkyl hypophosph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B03D1/001B03D1/02C12N1/20C12R1/01B03D103/02B03D101/02B03D101/06B03D103/04
CPCB03D1/001B03D1/02B03D2201/007B03D2201/02B03D2201/06B03D2203/025B03D2203/04C12N1/20
Inventor 蓝丽红郑锡瀚杨卓马忻狄蓝平
Owner GUANGXI UNIV FOR NATITIES
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