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Preparation method of ratiometric fluorescent lactate probe based on novel nanocomposite system and enzyme-catalyzed reaction

Active Publication Date: 2021-03-23
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no domestic and foreign literature and patent reports on the quantitative detection of lactic acid using the dual-signal peak intensity ratio method

Method used

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  • Preparation method of ratiometric fluorescent lactate probe based on novel nanocomposite system and enzyme-catalyzed reaction
  • Preparation method of ratiometric fluorescent lactate probe based on novel nanocomposite system and enzyme-catalyzed reaction

Examples

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Comparison scheme
Effect test

Embodiment 1

[0017] The preparation method of a ratiometric fluorescent lactic acid probe based on a novel nanocomposite system and enzyme-catalyzed reaction involved in this example and the principle schematic diagram of its quantitative detection of lactic acid are as follows figure 1 As shown, the specific preparation steps are as follows:

[0018] Preparation of sulfur quantum dots SQDs: Weigh 1g sublimated sulfur, 2mL polyethylene glycol PEG400 and 3g sodium hydroxide, add 50mL double distilled water under magnetic stirring to form a homogeneous mixed solution; transfer to 100mL polytetrafluoroethylene In an ethylene-lined micro-autoclave, stir and react at 70°C for 6h; after the reaction, take 2mL of the product solution, add 2mL of hydrogen peroxide aqueous solution with a mass concentration of 7wt%, and stir well to mix evenly to prepare SQDs The aqueous dispersion is ready for use;

[0019]Preparation of metal-organic framework composite SQDs / MOF coated in sulfur quantum dots: di...

Embodiment 2

[0023] The preparation method of a ratiometric fluorescent lactic acid probe based on a novel nanocomposite system and enzyme-catalyzed reaction and the schematic diagram of the principle for quantitative detection of lactic acid involved in this example are as follows figure 1 As shown, the preparation steps of SQDs, SQDs / MOF and BSA / AuNCs are the same as in Example 1, and other specific preparation steps are as follows:

[0024] Prepare a 6mg / mL SQDs / MOF aqueous dispersion and a 6mg / mL BSA / AuNCs aqueous dispersion; under magnetic stirring, fully mix 6mL SQDs / MOF aqueous dispersion and 6mL BSA / AuNCs aqueous dispersion , to form a homogeneous mixed solution; to the mixed solution, add a concentration of 10mmol / L ferric nitrate aqueous solution 2mL, then add 1.2mL concentration of 6U / mL lactate oxidase LOD solution, fully stir and mix to form a mixed solution system; Lactic acid LA was added to the mixed solution system, where the concentration of LA was adjusted to 0.1 μmol / L ...

Embodiment 3

[0026] The preparation method of a ratiometric fluorescent lactic acid probe based on a novel nanocomposite system and enzyme-catalyzed reaction and the schematic diagram of the principle for quantitative detection of lactic acid involved in this example are as follows figure 1 As shown, the preparation steps of SQDs, SQDs / MOF and BSA / AuNCs are the same as in Example 1, and other specific preparation steps are as follows:

[0027] Prepare the aqueous dispersion with a concentration of 8mg / mL SQDs / MOF and the aqueous dispersion with a concentration of 8mg / mL BSA / AuNCs; under magnetic stirring, fully mix 8mL of SQDs / MOF aqueous dispersion with 8mL of BSA / AuNCs aqueous dispersion , to form a homogeneous mixed solution; to the mixed solution, add a concentration of 10mmol / L ferric nitrate aqueous solution 4mL, then add 1.5mL concentration of 8U / mL lactate oxidase LOD solution, fully stir and mix to form a mixed solution system; Lactic acid LA was added to the mixed solution system...

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Abstract

The invention discloses a method for preparing a ratiometric fluorescent lactic acid probe based on a novel nanocomposite system and an enzyme-catalyzed reaction, preparing metal-organic framework composites SQDs / MOF coated within sulfur quantum dots, and gold nanoclusters BSA stabilized by bovine serum albumin / AuNCs, formulated SQDs / MOF, BSA / AuNCs, lactate oxidase LOD and Fe 2+ The mixed solution system, after adding lactic acid LA, LOD catalyzes LA to generate H 2 o 2 , H 2 o 2 Catalytic Fe 2+ into Fe 3+ , Fe 3+ The induced electron transfer causes the fluorescence of AuNCs to decrease, while the fluorescence of SQDs encapsulated in the MOF structure changes little. AuNCs / I SQDs Based on the linear relationship with the logarithm of LA concentration, a novel ratiometric fluorescent nanoprobe was constructed for highly sensitive and selective quantitative detection of lactate.

Description

technical field [0001] The invention belongs to the technical field of preparation of novel fluorescent nanocomposites and ratiometric fluorescent nanoprobes, and specifically relates to a method for preparing ratiometric fluorescent lactic acid probes based on novel nanocomposite systems and enzyme-catalyzed reactions. The prepared probes can be used in biological Highly sensitive and selective quantitative detection of lactate in fluid samples. Background technique [0002] Lactic acid (LA) is one of the final products of glycolysis in the human body. When oxygen is insufficient, reduced coenzyme I will accumulate, and then the glycolysis pathway will be activated, leading to the production of lactic acid. Human lactic acidosis is the most common type of metabolic acidosis, and the increase of lactic acid concentration in the blood is closely related to respiratory diseases and diabetic ketosis. In addition, bacterial meningitis, traumatic brain injury, etc. can also caus...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/56C09K11/02C09K11/58B82Y20/00B82Y30/00B82Y40/00
CPCB82Y20/00B82Y30/00B82Y40/00C09K11/02C09K11/56C09K11/58G01N21/6428G01N2021/6432
Inventor 金辉姜晓文桂日军
Owner QINGDAO UNIV
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