A kind of mutant enzyme for synthesizing monoglucose diglyceride and its preparation method and application

A technology of diglyceride and mutant is applied in the field of bioengineering, which can solve the problems of low synthesis reaction efficiency, low content of glyceroglycolipids, and many synthesis steps, and achieve the effects of good industrial application prospect, high conversion rate and high purity.
CN111518784BActive Publication Date: 2022-04-15ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
Publication Date
2022-04-15

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Abstract

The invention belongs to the field of bioengineering, in particular to a mutant enzyme for synthesizing monoglucose and diglyceride, a preparation method and application thereof. The nucleotide sequence of the mutant is shown in SEQ ID NO.1, and the amino acid sequence edited by nucleotides is shown in SEQ ID No.2. The marine bacterial glyceroglycolipid synthase mutant T257S prepared by the present application has good transglycosylation catalytic activity, and can efficiently convert UDP-glucose and diglyceride (sn-glycerol-1-stearate-2-oleate ), the conversion rate of monoglucose diglyceride reaches 68.5%, and the prepared monoglucose diglyceride has a high purity of more than 93.8%. It is suitable for the bioenzymatic preparation of glycolipid compound monoglucose diglyceride and has good industrial application prospect.
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Description

technical field

[0001] The invention belongs to the field of bioengineering, in particular to a mutant enzyme for synthesizing monoglucose and diglyceride, a preparation method and application thereof. Background technique

[0002] Glyceroglycolipids are compounds in which sugars are linked to glycerolipids by glycosidic bonds, and mainly exist in seaweeds, cyanobacteria and higher plants. The basic structure of glyceroglycolipids consists of a 1,2-diacyl-sn-glycerol moiety, and its monosaccharide or oligosaccharide is attached to the sn-3 position of the glycerol backbone, mainly including monogalactosyl diacylcerol (monogalactosyl diacylcerol, MGal-DAG), monoglucosyl diacylcerol (MGlc-DAG), monogalactosyl monoacylglycerol (MGMG), digalactosyldiacylglycerol (DGDG) and thioisorhamna Sugar diglycerol (sulfoquinovosy ldiacylglycerol, SQDG), etc. Glyceroglycolipids have important biological activities, such as antitumor, antiviral and anti-inflammatory activities, and related...

Claims

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