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Method for detecting liquid quality of antipsychotic drug in serum or plasma

A detection method and technology of psychotropic drugs, applied in the field of medical inspection and analysis, to achieve high accuracy of results, accurate qualitative and quantitative results, simple and reliable operation

Inactive Publication Date: 2020-08-11
大连润生康泰医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gas chromatography has the characteristics of high separation efficiency, fast analysis speed, and small sample volume, but the scope of application is only used for qualitative and quantitative analysis of gases and volatiles

Method used

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  • Method for detecting liquid quality of antipsychotic drug in serum or plasma
  • Method for detecting liquid quality of antipsychotic drug in serum or plasma
  • Method for detecting liquid quality of antipsychotic drug in serum or plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The recovery rate experiment of 8 kinds of antipsychotic drugs in serum:

[0041] 1. Materials and reagents

[0042] Chromatographic column: Kinetex C18 5μm, 50mm×3.0mm

[0043] Risperidone, clozapine, haloperidol, quetiapine and ziprasidone were purchased from China Institute for Food and Drug Control; paliperidone, quetiapine fumarate-d8 and ziprasidone-d8 were purchased From SIGMA; risperidone-d4, aripiprazole-d8, aripiprazole, olanzapine, olanzapine-d8, clozapine-d4, haloperidol-d4 and -hydroxyrisperidone- d4 was purchased from Cerilliant; methanol, acetonitrile, isopropanol, formic acid, ammonium formate (chromatographically pure), ammonia (chromatographically pure); ultrapure water: prepared by a Mili-Q ultrapure water machine.

[0044] 2. Instruments and equipment

[0045] High performance liquid chromatography-tandem mass spectrometer, equipped with electrospray ionization (ESI) ionization source (TRIPLE QUAD 4500MD, AB SCIEX, USA), liquid chromatographic separation mod...

Embodiment 2

[0070] 1. Materials and reagents

[0071] The difference from Example 1 is that the protein precipitation agent used in the pretreatment process is reconfigured according to the ratio of methanol: pure water (3:1);

[0072] 2. Apparatus and equipment

[0073] High performance liquid chromatography-tandem mass spectrometer, equipped with electrospray ionization (ESI) ionization source (API 3200MD, AB SCIEX, USA), liquid chromatographic separation mode is reversed phase chromatography, and the detector is triple quadrupole tandem mass spectrometry; One part electronic analytical balance; SPE solid phase extraction device.

[0074] 3. Matrix effect of 8 antipsychotic drugs in serum (without solid phase extraction pretreatment):

[0075] The pre-processing steps for detecting the matrix effect of 8 antipsychotic drugs by high performance liquid chromatography-tandem mass spectrometry are as follows:

[0076] 1) Serum extract + standard: Take 100μL of blank serum into a 1.5mL centrifuge tube...

Embodiment 3

[0105] Compare the extraction of 8 antipsychotic drugs with different types of commercial solid phase extraction columns C18, C18Phe and specific purification solid phase extraction columns.

[0106] 1. Materials and reagents

[0107] Commercial solid phase extraction column C18, C18Phe, specific purification packing.

[0108] 2. Apparatus and equipment

[0109] Same as Example 2.

[0110] 3. High performance liquid chromatography-tandem mass spectrometry detection of different types of solid phase extraction columns for the pretreatment of 8 antipsychotic drugs, the steps are:

[0111] 1) Add 100μL of human serum + 5μL of quality control (M) into a 1.5mL centrifuge tube, add 300μL of protein precipitation agent (acetonitrile: water = 3:1), vortex to mix, centrifuge at low temperature and high speed at 13500rpm for 5min, take the supernatant for use , In triplicate;

[0112] 2) Packing solid phase extraction column: Packing C18, C18Phe and the specific purification material of the presen...

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Abstract

The invention relates to a method for detecting the liquid quality of an antipsychotic drug in serum or plasma and pretreatment of a specific purification material of the antipsychotic drug. The method comprises the following steps: a serum or plasma sample is subjected to protein precipitation by a certain proportion of organic reagents; a supernatant obtained through centrifugal precipitation ispretreated with a specific purification material to obtain a purified sample solution, the purified sample solution is subjected to liquid chromatography-tandem mass spectrometry detection and analysis, and eight antipsychotic drugs including olanzapine, clozapine, quetiapine, aripiprazole, flupiperidinol, risperidone, pariperidone and ziprasidone in serum or plasma are detected at the same time.The pretreatment of the specific purification material is a mixed solid-phase extraction column taking silica gel as a matrix, and the blood is purified by the specific purification material, so thatthe interference of the matrix in the blood can be removed, the matrix effect of eight antipsychotic drugs can be improved, and the recovery rate of the antipsychotic drugs can be better ensured.

Description

Technical field [0001] The invention belongs to the technical field of medical testing and analysis, and specifically relates to a detection of antipsychotic drugs olanzapine, haloperidol, paliperidone, aripiprazole, risperidone, clozapine in serum or plasma samples , Quetiapine fumarate and ziprasidone mesylate by liquid chromatography-tandem mass spectrometry. [0002] technical background [0003] Therapeutic drug concentration monitoring refers to the measurement of the concentration of a specific drug in the patient's blood at specified time intervals during clinical drug treatment. Antipsychotic drugs are also called nerve blockers. Except for schizophrenia and paranoid mental disorders, schizophrenia and other mental disorders with psychomotor excitement delusions and fantasies are all indications. At present, there are many types of antipsychotic drugs, including typical drugs and atypical drugs that have been used for a long time. Typical drugs are mainly good for positiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/89
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/89G01N2030/062G01N2030/324
Inventor 曹云峰王爽张亚莲于杰潘永强洪沫
Owner 大连润生康泰医学检验实验室有限公司
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