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Application of the mixture of Lactobacillus plantarum klds1.0386 and tryptophan in the preparation of drugs for preventing colitis

A technology of KLDS1.0386 and Lactobacillus plantarum, which is applied in the field of medicine, can solve problems such as limited strain safety cannot be guaranteed, and achieve the effects of good growth performance, good probiotic function, strong acid production ability and acid and bile salt resistance

Active Publication Date: 2020-11-27
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ability of intestinal microorganisms to decompose and utilize tryptophan is limited. Current studies have found that Escherichia coli, Clostridium, Bacteroides, and Peptostreptococcus have the function of degrading tryptophan. Enterococcus strains can use tryptophan, but Enterococcus is a potentially pathogenic pathogenic bacteria, the safety of the strains to the human body cannot be guaranteed

Method used

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  • Application of the mixture of Lactobacillus plantarum klds1.0386 and tryptophan in the preparation of drugs for preventing colitis
  • Application of the mixture of Lactobacillus plantarum klds1.0386 and tryptophan in the preparation of drugs for preventing colitis
  • Application of the mixture of Lactobacillus plantarum klds1.0386 and tryptophan in the preparation of drugs for preventing colitis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1 Materials and methods

[0041] 1.1 Experimental materials

[0042] 1.1.1 Tested strains

[0043] Lactobacillus plantarum (KLDS1.0317, KLDS1.0318, KLDS1.0386, KLDS1.0344, 1-2, 2-2, 4-5, 1-5, 4-4, 8-6), Lactobacillus acidophilus ( KLDS1.0901, KLDS1.0902, KLDS1.1003), Lactobacillus rhamnosus (1.0911, 1.0912, LGG), were all provided by the Industrial Microorganism Culture Collection Center (KLDS-DICC), the Key Laboratory of Dairy Science and Education.

[0044] 1.1.2 Experimental animals

[0045] Clean grade C57BL / 6N mice, male, 8 weeks old, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., license number: SCXK (Beijing) 2016-0006.

[0046] 1.1.3 Medium

[0047] 1.1.3.1 MRS medium

[0048] 5.0g peptone, 10.0g tryptone, 5.0g sodium acetate, 5.0g yeast extract, 20.0g glucose, 1.0g Tween-80, 0.25g manganese sulfate, 2.0g diammonium hydrogen citrate, 0.58g magnesium sulfate, phosphoric acid Dipotassium hydrogen dipotassium 2.0g, beef ex...

Embodiment 2

[0193] The difference between this embodiment and embodiment 1 is only: every 10 8 0.2 mg of tryptophan is contained in 0.2 mL of CFU / mL Lactobacillus plantarum suspension.

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Abstract

The invention discloses the application of the mixture of Lactobacillus plantarum KLDS1.0386 and tryptophan in the preparation of medicaments for preventing colitis. It specifically discloses the application of the mixture of Lactobacillus plantarum KLDS1.0386 and tryptophan in the preparation of drugs for reducing the activity of colon tissue myeloperoxidase MPO, the application of drugs in the preparation of drugs for improving the immune response disorder of colitis, and the preparation of drugs for protecting intestinal The application in the preparation of drugs for intestinal barrier, the application in the preparation of drugs for protecting intestinal mucosa, and the application in the preparation of drugs for regulating intestinal microorganisms. The present invention evaluates the degradation ability of 16 strains of Lactobacillus to tryptophan in vitro, and screens out Lactobacillus plantarum KLDS1.0386 with higher ability to degrade tryptophan; Lactobacillus plantarum KLDS1.0386 has good growth performance and strong production capacity. Acid resistance and acid and bile salt resistance, showing good probiotic function and colitis prevention function.

Description

technical field [0001] The invention relates to the application of a mixture of Lactobacillus plantarum KLDS1.0386 and tryptophan, in particular to the application of a mixture of Lactobacillus plantarum KLDS1.0386 and tryptophan in the preparation of drugs for preventing colitis, belonging to the technical field of medicine. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a chronic and relapsing autoimmune disease, including ulcerative colitis (ulcerative colitis, UC) and Crohn's disease (Crohn's disease, CD) two disease types . In the past, IBD was considered to be a western disease, mainly concentrated in developed countries such as North America, Europe, Australia, and New Zealand. In recent years, the incidence of IBD in emerging industrial countries such as Asia and the Middle East has risen sharply, and it has developed into a global disease. The clinical manifestations of IBD are diarrhea, hematochezia, and weight loss. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/00A61K31/405
CPCA61K31/405A61K35/747A61P1/00A61K2300/00Y02A50/30
Inventor 李柏良王娜娜李娜闫芬芬刘飞王松霍贵成
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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