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Method of extracting thaumatin from Thaumatococcus daniellii based on microbial fermentation

A technology of microbial fermentation and arrowroot, applied in the biological field, can solve the problems of insufficient extraction of thaumatin, complicated method steps, and significant environmental pollution, and achieve the goal of increasing extraction yield, low extraction cost, and reduced extraction yield Effect

Active Publication Date: 2020-08-25
WUHAN HUASWEET
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Through comparative studies, it has been found that the reported method steps are complicated, the sweetness loss rate of thaumatin is as high as more than 55%, the extraction cost is high, and the environmental pollution is significant, and the arrowroot fruit in the traditional extraction method all removes the stone seeds, while the arrowroot fruit Nuclei are also rich in thaumatin, but the existing technology for extracting thaumatin from African arrowroot can not fully extract thaumatin in arrowroot fruit

Method used

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  • Method of extracting thaumatin from Thaumatococcus daniellii based on microbial fermentation
  • Method of extracting thaumatin from Thaumatococcus daniellii based on microbial fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Wash the arrowroot, cut it into pieces, add 1 times the weight of the African arrowroot fresh fruit, add 0.5% aqueous glycerin solution twice the weight of the African arrowroot fresh fruit, and beat the pulp and core together;

[0032] (2) Simultaneously inoculate Aspergillus niger ATCC 16404, ATCC 6275 and Bacillus subtilis ATCC6051, ATCC 9327 in the slurry, ferment at 40°C for 12 hours; the seed medium components of Aspergillus niger ATCC 16404 and ATCC 6275 are 0.1% carbonic acid Calcium, 0.05% magnesium chloride, 0.05% ferrous chloride, 0.1% sodium chloride, 0.2% beef extract, 0.1% peptone, 0.1% yeast powder, 2% fructose, 1% glycerin, culture temperature 30 ℃; Bacillus subtilis ATCC The seed medium components of 6051 and ATCC 9327 are 0.3% beef extract, 0.5% peptone, 0.3% yeast powder, 0.5% glycerol, 0.2% calcium carbonate, 0.1% magnesium chloride, 0.5% sodium chloride, and the culture temperature is 35°C. The inoculation amounts of Aspergillus niger ATCC 16404...

Embodiment 2

[0040] (1) Wash the arrowroot, cut it into pieces, add 2 times the weight of the African arrowroot fresh fruit, add 1% glycerin aqueous solution with 3 times the African arrowroot fresh fruit quality, and beat the pulp and core together;

[0041] (2) Simultaneously inoculate Aspergillus niger ATCC 16404, ATCC 6275 and Bacillus subtilis ATCC6051, ATCC 9327 in the slurry, fermentation temperature 25 ℃, fermentation 24 hours; The seed medium component of Aspergillus niger ATCC 16404 and ATCC 6275 is 0.15% carbonic acid Calcium, 0.08% magnesium chloride, 0.08% ferrous chloride, 0.2% sodium chloride, 0.25% beef extract, 0.15% peptone, 0.15% yeast powder, 2.5% fructose, 1.5% glycerin, culture temperature 31 ℃; Bacillus subtilis ATCC The seed medium components of 6051 and ATCC 9327 are 0.4% beef extract, 0.6% peptone, 0.5% yeast powder, 0.7% glycerol, 0.25% calcium carbonate, 0.12% magnesium chloride, 0.6% sodium chloride, and the culture temperature is 36°C. The inoculation amounts ...

Embodiment 3

[0049] (1) Arrowroot is washed, cut into pieces, ice with 1.5 times the quality of the fresh Arrowroot African fruit, and 0.7% glycerin aqueous solution with 2.5 times the fresh fruit quality of Arrowroot African Arrowroot are added, and the pulp and core are beaten together;

[0050] (2) Simultaneously inoculate Aspergillus niger ATCC 16404, ATCC 6275 and Bacillus subtilis ATCC6051, ATCC 9327 in the slurry, the fermentation temperature is 32 ℃, and ferment for 16 hours; the seed medium component of Aspergillus niger ATCC 16404 and ATCC 6275 is 0.2% carbonic acid Calcium, 0.1% magnesium chloride, 0.1% ferrous chloride, 0.2% sodium chloride, 0.2% beef extract, 0.2% peptone, 0.2% yeast powder, 3% fructose, 1.5% glycerin, culture temperature 32°C; Bacillus subtilis ATCC The seed medium components of 6051 and ATCC 9327 are 0.5% beef extract, 0.7% peptone, 0.5% yeast powder, 0.8% glycerol, 0.3% calcium carbonate, 0.15% magnesium chloride, 0.8% sodium chloride, and the culture temper...

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Abstract

The invention discloses a method of extracting thaumatin from Thaumatococcus daniellii based on microbial fermentation. As the thaumatin in Thaumatococcus daniellii fruits is combined with polysaccharides like cellulose, the effect of extracting the thaumatin is greatly impacted. According to the invention, through fermenting aspergillus niger and bacillus subtilis in a proper fermentation condition, cellulase and amylase for degrading the cellulose and the polysaccharides can be synthesized to eliminate the impact of polysaccharide substances on thaumatin extraction. And meanwhile, the thaumatin contained in the kennel and flesh of the Thaumatococcus daniellii fruits is also extracted.. Therefore, according to the method provided by the invention, extraction steps are greatly reduced; extraction cost is low; environmental pollution is less; and the loss rate of thaumatin sweetness can be controlled within the range of 16%-28%. Meanwhile, extraction yield is increased as the yield of the thaumatin is increased by 22%-31% compared to that of traditional thaumatin extraction just from the fruit flesh.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a production technology for cleanly extracting thaumatin from African arrowroot based on microbial fermentation. Background technique [0002] Thaumatin is a natural sweetening protein extracted from African arrowroot. It has the characteristics of high sweetness, low calorie, safety and non-toxicity. It is a new type of sweetener with high sweetness and no sugar. Thaumatin can be used in foods such as cigarettes, drinks, beverages and candies, and can meet the sweetness needs of patients with high blood pressure, diabetes and obesity. Thaumatin can also be used as a feed additive to improve the palatability of feed, increase the appetite of livestock, and improve the production efficiency of livestock. Thaumatin can also be used as an excipient for medicines, which reduces the bitterness of medicines and facilitates the use of medicines, especially for infants and child...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/43C12P21/02C07K1/16C12R1/125C12R1/685
CPCC07K14/43C12P21/02
Inventor 谢新春王红波胡思前王亚珍陈芃菲周飞邑谢一溉
Owner WUHAN HUASWEET
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