Chitosan-based nerve fiber membrane, preparation method, nerve conduit and application

A nerve fiber and nerve conduit technology, applied in the field of nerve tissue engineering materials, can solve the problems of easy irritation of degradants, unfavorable local inflammation in the body, and poor mechanical properties, and achieve suitable biodegradability, excellent mechanical strength, and good mechanical properties. Effect

Active Publication Date: 2020-09-08
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at problems such as poor mechanical properties and degradation products that easily stimulate the body to cause local inflammation and are

Method used

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  • Chitosan-based nerve fiber membrane, preparation method, nerve conduit and application
  • Chitosan-based nerve fiber membrane, preparation method, nerve conduit and application
  • Chitosan-based nerve fiber membrane, preparation method, nerve conduit and application

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preparation example Construction

[0070] The preparation method of described chitosan-based nerve fiber membrane comprises the following steps:

[0071] (1) Preparation of spinning solution: spinning solution includes A solution and B solution, wherein, A solution contains degradable functional materials, carbon nanotubes modified by hydrophilic functional groups, Lycium barbarum polysaccharide, and chitosan and / or chitosan Sugar derivative; B solution contains nerve growth factor and stabilizer.

[0072] The concentration of each component in the A solution and the B solution should ensure that the total content of the composite nanofiber mass content of the orientation arrangement obtained at last is 100% and the content of each component meets the following conditions:

[0073]

[0074] In some embodiments, the concentration of each component of the above-mentioned solution A is respectively:

[0075]

[0076] The solvent used in solution A may be hexafluoroisopropanol (English abbreviation: HFIP). ...

Embodiment 1

[0090] A preparation method of chitosan-based nerve fiber membrane, which comprises the following steps:

[0091] (1). With 1.25g polylactic acid-glycolic acid copolymer (PLGA, wherein the mass ratio of lactic acid monomer and glycolic acid monomer is 75:25), 0.35g chitosan (CS, deacetylation degree is 90%) Dissolve 10 mg of Lycium barbarum polysaccharide (LBP) in 5 mL of hexafluoroisopropanol at the same time, and stir magnetically for 24 hours to fully dissolve PLGA and LBP to form a uniform solution.

[0092] (2). Add 0.1 g carboxy-modified multi-walled carbon nanotubes (MWCNTs, average diameter 15 nm, average length 1 μm) and 100 μL Span 80 to the solution obtained in step (1), and stir magnetically for 8 hours to disperse the MWCNTs uniformly In solution, A solution was obtained.

[0093] (3). Prepare 10 μg / mL Nerve Growth Factor (NGF) aqueous solution, and add bovine serum albumin (BSA) at the same time, control the concentration of BSA to 10 mg / mL to obtain B solution....

Embodiment 2

[0097] A preparation method of chitosan-based nerve fiber membrane, comprising the following steps:

[0098] (1). 0.9g polylactic acid-glycolic acid copolymer (PLGA, wherein the mass ratio of lactic acid monomer and glycolic acid monomer is 85:15), 0.32g chitosan (CS, deacetylation degree is 90%) and 10 mg Lycium barbarum polysaccharide (LBP) were dissolved in 2.5 mL hexafluoroisopropanol at the same time, and magnetically stirred for 24 hours to fully dissolve PLGA and LBP to form a uniform solution.

[0099] (2). Add 0.01 g carboxyl-modified multi-walled carbon nanotubes (MWCNTs, average diameter 10 nm, average length 5 μm) and 50 μL Span 80 to the solution obtained in step (1), and stir magnetically for 8 hours to disperse the MWCNTs uniformly In solution, A solution was obtained.

[0100] (3). Prepare 10 μg / mL Nerve Growth Factor (NGF) aqueous solution, and add bovine serum albumin (BSA) at the same time, control the concentration of BSA to 10 mg / mL to obtain B solution. ...

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Abstract

The invention relates to the technical field of nervous tissue engineering materials, and particularly provides a chitosan-based nerve fiber membrane, a preparation method, a nerve conduit and application. The chitosan-based nerve fiber membrane is formed by a plurality of composite nanofibers which are arranged in an directional manner; each composite nanofiber is of a core-shell structure, a shell layer of each nanofiber comprises a degradable functional material, a hydrophilic functional group modified carbon nanotube, lycium barbarum polysaccharide and chitosan and/or a chitosan derivative, and a core layer of each nanofiber comprises a nerve growth factor and a stabilizer. The chitosan-based nerve fiber membrane disclosed by the invention has the characteristics of good orientation arrangement, good mechanical property and high biological responsiveness; and during degradation, the chitosan and/or chitosan derivative hydrolysate and alkalinity of amino groups carried by the chitosan and/or the chitosan derivative hydrolysate can effectively neutralize weak acidity of degradation products, influences of acidic degradation products on the surrounding environment and tissues areavoided, and therefore the fiber membrane can be applied to nerve repair and regeneration of human bodies and animal bodies.

Description

technical field [0001] The invention belongs to the technical field of nerve tissue engineering materials, and in particular relates to a chitosan-based nerve fiber membrane, a preparation method, a nerve guide and application. Background technique [0002] In modern surgery, tissue repair membranes are often used clinically to repair tissues and organs. This requires tissue repair membranes to have good biocompatibility, certain strength, and facilitate cell adhesion and growth. [0003] In many surgical operations, a common condition is peripheral nerve defect, which often leads to the loss of sensory and motor functions in the corresponding innervated area of ​​the patient. Therefore, the repair and regeneration of peripheral nerve injury has always been a research hotspot in the field of clinical and neuroscience. . [0004] At present, the clinical treatment methods for repair and regeneration after nerve injury mainly include end-to-end suture, autologous or allogene...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/54A61L27/58D04H1/4326D04H1/728D04H1/76
CPCA61L27/58A61L27/54A61L27/20D04H1/4326D04H1/728D04H1/76A61L2400/12A61L2430/32A61L2300/414A61L2300/604C08L5/08
Inventor 赵晓丽刘缘王静潘浩波
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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