Preparation method and application of nematophagous fungus chlamydospore wettable powder
A wettable powder and nematode-eating fungus technology, which is applied in the field of animal and plant parasitic nematodes, can solve the problems of inability to store for a long time, achieve significant insecticidal effect, low technical difficulty, and convenient storage and transportation
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Embodiment 1
[0028] Embodiment 1 A kind of preparation method of nematode fungus chlamydospore wettable powder
[0029] Step 1, nematode fungi D. flagrans Isolation, purification and identification of isolate SDH035
[0030] In September 2013, samples from sheep manure piles in Wuzhong area of Ningxia and sheep manure samples in Yichun City, Heilongjiang Province were separated by the improved plate spreading method. During the separation, the third stage larvae of sheep nematodes were added to the plate as bait, 25 Incubate at ℃ temperature, check once a day or every other day under an inverted microscope, and last for 3 weeks. After conidia grow, pick a single conidia and place it in another 0.2% bran single predatory structure or by The preyed nematodes were transferred to 0.2% bran agar medium and cultured at 25°C until a pure culture was obtained. The morphology and data measurement of the conidia of the strain were obtained by the slide insert method, and the strain DNA was extr...
Embodiment 2
[0037] The determination experiment of drying condition in the preparation step of embodiment 2 chlamydospore powder
[0038] Two drying methods, drying oven drying and vacuum drying oven drying, were used for experiments, and the temperature was set at 35 °C, 40 °C and 45 °C for each method; at the same time, the dry chlamydospore powder was placed at 4 °C Below is contrast, and concrete drying condition is as shown in table 1;
[0039]
[0040] When above-mentioned temperature drying finishes, calculate spore germination rate respectively;
[0041] The calculation method for calculating the spore germination rate is as follows: take the dried spore powder and add distilled water for 10-fold dilution, take 100 μL of the diluted spore liquid and spread it evenly on the plane of the water agar plate, set 3 parallels for each experiment, and place the plate at 28 Cultivate at ℃ for 36 hours, count the number of germinated spores and ungerminated spores respectively, and the ...
Embodiment 3
[0051] Example 3 Wetting agent screening experiment of nematode fungus chlamydospore wettable powder
[0052] Sodium dodecylsulfonate, sodium dodecylbenzenesulfonate, and pull-off powder are used as wetting agents, and D. flagrans Chlamydospore powder and diatomaceous earth were evenly mixed to obtain test samples, the proportions of wetting agents in the test samples were 2%, 4% and 8% (w / w), and the proportion of diatomite in the test samples Be 20%; Take the chlamydospore powder without wetting agent as contrast; Carry out following experiment:
[0053] Take a beaker with a capacity of 150 mL and add 100 mL of tap water, weigh 0.1 g of the above-mentioned sample, and pour it evenly on the sample liquid from the position where the beaker is flush with the mouth of the beaker, and immediately use a stopwatch to count until the sample is completely wet. Record the time. Repeat 5 times to get the average value. The experimental conditions and experimental results are shown ...
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