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Intestine-targeted oral probiotic preparation and preparation method and application thereof

A probiotic preparation and a technology for probiotics are applied in the directions of medical preparations without active ingredients, medical preparations containing active ingredients, pharmaceutical formulas, etc., which can solve the problem that the survival rate of probiotics needs to be improved, and achieve good biological safety. and biocompatibility, dense coating effect, simple, quick and mild preparation process

Inactive Publication Date: 2020-09-29
佛山南溟生物医学材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent has improved the acid-resistant and bile-salt-resistant activities of probiotics to a certain extent, it failed to solve the problem of targeted release of probiotics in the intestinal tract, and the survival rate of probiotics still needs to be improved

Method used

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  • Intestine-targeted oral probiotic preparation and preparation method and application thereof
  • Intestine-targeted oral probiotic preparation and preparation method and application thereof
  • Intestine-targeted oral probiotic preparation and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] A preparation method of an oral probiotic preparation, comprising the following steps:

[0043] 1. After culturing the activated Streptococcus lactis to an OD value of 0.4, centrifuge to discard the supernatant and resuspend it with a 0.5% sodium alginate (molecular weight: 200,000) solution with a volume ratio of 1:10;

[0044] 2. Set up an electrostatic droplet device, set the high-voltage power supply voltage to 6000V, and the flow rate of the syringe pump to be 10ml / h, dropwise into the 0.1M calcium chloride solution;

[0045] 3. After solidifying for a period of time, discard the supernatant, wash twice with deionized water, add a salmontamine solution with a concentration of 2 mg / mL, and shake at 80 rpm for a period of 10 min to obtain calcium alginate microcapsules with one layer of salmontamine;

[0046]4. Add sodium alginate solution with a concentration of 0.2%, shake at 80 rpm for a period of 10 minutes, wash off the excess sodium alginate on the surface with...

Embodiment 2

[0050] A preparation method of oral probiotic preparation, comprising the following steps:

[0051] 1. After culturing the activated Lactobacillus plantarum to an OD value of 0.6, the supernatant was discarded by centrifugation, and resuspended with a 2.5% sodium alginate (molecular weight 10,000) solution with a volume ratio of 1:20;

[0052] 2. Set up an electrostatic droplet device, set the high-voltage power supply voltage to 4500V, and the flow rate of the syringe pump to be 12ml / h, dropwise into the 1M calcium chloride solution;

[0053] 3. After solidifying for a period of time, discard the supernatant, wash three times with deionized water, add a 5 mg / mL salmontamine solution, and shake at 150 rpm for a period of 20 min;

[0054] 4. Aspirate the supernatant, wash it twice with deionized water, and add it to 1% sodium alginate and 0.1M calcium chloride solution in turn;

[0055] 5. Repeat steps 1-2 times to obtain calcium alginate microspheres with 1, 2, and 3 layers o...

Embodiment 3

[0058] A preparation method of oral probiotic preparation, comprising the following steps:

[0059] 1. After culturing the activated bifidobacteria to an OD value of 0.5, centrifuge to discard the supernatant, and resuspend with a 2% sodium alginate (molecular weight: 100,000) solution with a volume ratio of 1:15;

[0060] 2. Set up an electrostatic droplet device, set the high-voltage power supply voltage to 5000V, and the flow rate of the syringe pump to be 10mL / h, dropwise into the 0.1M calcium chloride solution;

[0061] 3. After solidifying for a period of time, discard the supernatant, wash three times with deionized water, add a solution of salmontamine with a concentration of 2.5 mg / mL, and shake at 100 rpm for a period of 15 minutes;

[0062] 4. Aspirate the supernatant, wash it twice with deionized water, and add it to 0.5% sodium alginate and 0.1M calcium chloride solution in turn;

[0063] 5. Repeat steps 1-2 times to obtain calcium alginate microspheres with 1, 2...

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Abstract

The invention discloses an intestine-targeted oral probiotic preparation and a preparation method and application thereof. The core of the preparation is a calcium alginate gel inner core containing probiotics, the inner core is alternately coated with basic amino acid polymer layers and a calcium alginate layer from the inner core to the outside, and the outermost layer is the basic amino acid polymer layers; and the preparation contains at least two basic amino acid polymer layers. The microcapsule adopts a multi-layer structure, compared with a single-layer microcapsule, the microcapsule has a denser coating effect and can well embed microorganisms; the microcapsule has better acid resistance, can resist the invasion of gastric acid and bile salt, and significantly improves the survivalrate of bacteria. The multi-layer microcapsule uses a basic amino acid polymer as the outer coating layer, which does not inhibit the disintegration of the microcapsule, but can release the microorganisms in the end of a small intestine quickly, accurately and efficiently, and has excellent targeting ability.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to an intestinal-targeted oral probiotic preparation and a preparation method and application thereof. Background technique [0002] Probiotics are widely found in the human intestine and other parts, and are an important part of the human intestinal microecology. Probiotics have been confirmed to be closely related to the physiological functions of the human body. In addition to improving intestinal health and promoting nutrient absorption, they are also closely related to diabetes, cardiovascular disease, liver disease, tumors and other diseases. Therefore, it is of great significance to human health to precisely deliver probiotics to colon and other parts and colonize them, thereby regulating the balance of flora in the intestinal tract, and then regulating the immune function of the host mucosa and system. [0003] At present, the main ways for people to actively...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K35/744A61K35/747A61K35/745A61K47/36A61K47/42A61K47/34A61P1/00
CPCA61K9/0002A61K9/5031A61K9/5036A61K9/5052A61K9/5073A61K35/744A61K35/745A61K35/747A61K47/36A61P1/00
Inventor 程启坤蔡祥杨德慧
Owner 佛山南溟生物医学材料有限公司
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