Fluorescent endoscope imaging system and method for high efficiency transmission based on double channels
An imaging system and endoscope technology, applied in the field of endoscopes, can solve problems such as inability to accurately and synchronously focus dual-mode images, difficulty in optimizing registration speed and reliability, incomplete suppression of stray light interference, etc., to achieve suppression of stray light Interference, reduced image processing speed, compact effect
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Embodiment 1
[0045] according to Figure 8 , which shows the near-infrared fluorescence grayscale image and the green fluorescence image after pseudo-color processing of the resected lesion tissue specimen during the operation collected under dual-channel fluorescence endoscopic imaging.
[0046] Tissue samples are initially stained with ICG targeting probes, which are targetedly combined with ICG fluorescent molecules in the lesion area and enriched for a long time. A dual-channel fluorescence endoscopic imaging device was used for light excitation and image acquisition. The excitation light selects 760nm ± 20nm near-infrared LED light source, the optical power is 120mW at the input end of the light guide joint 6, and the excitation light is passed through f =8mm collimating lens 24, and then output from the endoscope objective lens through the fiber bundle, the measured optical power density at the working distance of 5cm is 8.5mW / cm 2 . The part of the tissue sample combined with the...
Embodiment 2
[0051] like Figure 9 Shown is the synchronous focusing effect diagram of dual-mode image acquisition under the action of dual-channel fluorescence endoscope imaging. The samples were selected from lesion tissue specimens resected during the operation, the imaging endoscope device was selected from laparoscope, the fluorescent molecule was selected from ICG fluorescent dye, the excitation light source was selected from 780nm near-infrared LED, and the auxiliary lighting source was selected from white LED that meets medical standards (Luminus Devices, USA). , CBT-90-W57H-C11-KB201), COMS is a back-illuminated photosensitive chip.
[0052]Two sets of images are shown in the figure, the left one is a near-infrared fluorescence image (top half) and a visible light color image (bottom half) collected during preliminary assembly. Wherein, the endoscope fixed base 13, the cube dichroic prism 15, the partition bracket 16, the near-infrared COMS 17 and the three-dimensional tunable co...
Embodiment 3
[0054] like Figure 10 Shown is the fusion calibration rendering of dual-mode images. The sample is the ICG dye diluted with sterilized distilled water, and put into the EP tube. The imaging endoscope device adopts the double-channel high-efficiency transmission fluorescent laparoscope imaging device designed by the present invention. The effect picture shows that the fluorescent grayscale image is processed by pseudo-color, and the R and B basic colors are removed, and only the G basic color is retained. The RGB color value ratio is 0: 255: 0. The processed green fluorescent image uses the nonlinear conjugate gradient method. Point-to-point mapping to the color image collected by visible light CMOS, so as to obtain the dual-mode information superposition fusion image of the same part and the same moment.
[0055] The left side of the figure is the dual-mode fusion effect diagram after preliminary assembly according to the size of the workpiece. From the outline of the EP tub...
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