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Photoelectrochemical sensor preparation method based on efficient and stable in-situ growth of titanium dioxide/nanogold photo-anode

An in-situ growth, titanium dioxide technology, applied in biochemical equipment and methods, nanotechnology for sensing, nanotechnology, etc., can solve the problems that have not yet been systematically carried out, large batch differences, poor electrode repeatability, etc.

Pending Publication Date: 2020-10-20
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although for ITO / TiO 2 The PEC sensor sensing strategy of the photoanode has been continuously improved, and the corresponding PEC sensor has achieved a lot of results in the analysis, detection and sensing of biomacromolecules. However, when optimizing TiO 2 layer, not much work has been done systematically
In particular, the current TiO 2 layer mainly by drop-coating TiO 2 Yes, but it will cause adverse effects such as uneven photoanode substrates, large batch differences, and poor electrode repeatability. Therefore, it is very necessary to develop efficient, uniform, stable and reliable ITO / TiO 2 photoelectrochemical electrode

Method used

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  • Photoelectrochemical sensor preparation method based on efficient and stable in-situ growth of titanium dioxide/nanogold photo-anode
  • Photoelectrochemical sensor preparation method based on efficient and stable in-situ growth of titanium dioxide/nanogold photo-anode
  • Photoelectrochemical sensor preparation method based on efficient and stable in-situ growth of titanium dioxide/nanogold photo-anode

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Embodiment 1

[0027] The photoelectrochemical sensor preparation method based on efficient and stable in-situ growth of titanium dioxide / nano-gold photoanode in this example includes three parts: (1) in-situ preparation of efficient and stable titanium dioxide / nano-gold photoanode; (2) construction of miRNA recognition unit (3) miRNA detection application of photochemical sensor.

[0028] (1) In situ preparation of highly efficient and stable TiO / nano-gold photoanode

[0029] (1) Cleaning the ITO slices: First, put the ITO slices in acetone, ethanol, and ultrapure aqueous solution for 15-20 minutes respectively; Place the ITO side up in a petri dish and anneal at 120°C for 2-4 hours; finally, the ITO slices are taken out for plasma and ozone treatment to increase the work function of the ITO surface, and the formed surface structure is easily attached by the solution spin-coated organic compound .

[0030] (2) Spin-coat an appropriate amount of tetraisopropyl titanate (or butyl titanate, ...

Embodiment 2

[0044] The present embodiment provides the HRTEM image (scale bar, 5nm), ultraviolet-visible spectrum, fluorescence spectrum and dynamic light scattering diagram (DLS) of CdTe-COOH quantum dot solution, such as figure 1 shown. It is important to have a basic understanding of the physical properties of water-soluble quantum dots of CdTe-COOH as sensitizers for PEC sensors. figure 1 Panel A shows the HRTEM image of CdTe-COOH QDs. The dynamic light scattering (DLS) profile describes the size distribution of these quantum dots in deionized water ( figure 1 Figure C), the average diameter is about 3.0±0.8nm, and the distribution is relatively uniform. Such as figure 1 As shown in Figure B, the UV-Vis absorption spectrum of CdTe-COOH quantum dots shows an absorption peak at 481nm and an absorption edge at 530nm. In addition, the fluorescence emission spectrum of CdTe quantum dots has an emission peak at 526nm. MPA-modified CdTe-COOH quantum dots have good solubility in PBS buff...

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Abstract

The invention discloses a photoelectrochemical sensor preparation method based on efficient and stable in-situ growth of a titanium dioxide / nanogold photo-anode. The preparation method comprises the following steps: cleaning ITO (indium tin oxide) slices of an indium tin oxide electrode; sequentially putting the ITO slices into acetone, ethanol and an ultrapure water solution for ultrasonic treatment; wiping with an ethanol cotton ball, washing with ultrapure water, annealing in a culture dish with one side of ITO upward, taking out the ITO slices, and carrying out plasma and ozone treatment;spin-coating a tetraisopropyl titanate, butyl titanate or ethyl titanate precursor on the treated ITO slices, and then annealing a spin-coated ITO substrate to obtain an ITO / TiO2 electrode; and cooling the ITO / TiO2 electrode to room temperature, and carrying out in-situ gold nanoparticle loading to obtain an ITO / TiO2 / Au NPs photo-anode. The photoelectrochemical sensor has the advantages of being efficient, uniform, stable, high in preparation repeatability, simple in structure, good in storage stability, good in detection repeatability, sensitive to miRNA-21, specific to miRNA-21 and the like,and is used for detecting miRNA.

Description

technical field [0001] The invention relates to a method for preparing a photoelectrochemical sensor, in particular to a method for preparing a photoelectrochemical sensor based on high-efficiency and stable in-situ growth of titanium dioxide / nano-gold photoanode. Background technique [0002] miRNAs are a class of non-coding short (ca 18-25 nucleotide) RNAs that participate in a variety of biological processes, especially the abnormal expression of miRNAs in cancer patients is closely related to the occurrence, development and metastasis of tumors, which can be used in clinical Provide helpful clues. In recent years, miRNAs have become biomarkers of important diseases, so it is of great significance to develop simple, rapid, reliable and sensitive detection methods for miRNAs. Traditional miRNA detection methods include real-time reverse transcription polymerase chain reaction (RT-PCR), hybridization, gene chip and spectrophotometry. However, these techniques have the dis...

Claims

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Application Information

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IPC IPC(8): G01N27/327C12Q1/6825B82Y15/00B82Y40/00
CPCG01N27/3278C12Q1/6825B82Y15/00B82Y40/00C12Q2525/207C12Q2525/301C12Q2565/607
Inventor 傅妮娜田思雨汪联辉丁竹成王路璐章胜裕
Owner NANJING UNIV OF POSTS & TELECOMM
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