The invention discloses a photoelectrochemical sensor preparation method based on efficient and stable in-situ growth of a
titanium dioxide / nanogold photo-
anode. The preparation method comprises the following steps: cleaning ITO (
indium tin oxide) slices of an
indium tin oxide electrode; sequentially putting the ITO slices into
acetone,
ethanol and an
ultrapure water solution for ultrasonic treatment; wiping with an
ethanol cotton ball, washing with
ultrapure water, annealing in a culture dish with one side of ITO upward, taking out the ITO slices, and carrying out
plasma and
ozone treatment;spin-
coating a
tetraisopropyl titanate, butyl
titanate or ethyl
titanate precursor on the treated ITO slices, and then annealing a spin-coated ITO substrate to obtain an ITO / TiO2
electrode; and cooling the ITO / TiO2
electrode to
room temperature, and carrying out in-situ gold
nanoparticle loading to obtain an ITO / TiO2 / Au NPs photo-
anode. The photoelectrochemical sensor has the advantages of being efficient, uniform, stable, high in preparation
repeatability, simple in structure, good in storage stability, good in detection
repeatability, sensitive to miRNA-21, specific to miRNA-21 and the like,and is used for detecting miRNA.