Preparation method of cobamamide crude product

A technology for adenosylcobalamin and crude product, which is applied in the field of preparation of crude adenosylcobalamin, can solve the problems of generating a lot of waste water, affecting the continuity of operations, and many times of dismantling and washing.

Active Publication Date: 2020-10-23
NINGXIA KINGVIT PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to improve the yield, it is necessary to add water for many times to adjust the pulp and extract. The centrifuge is easy to be polluted, and the number of time

Method used

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  • Preparation method of cobamamide crude product

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Experimental program
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Effect test

Embodiment 1

[0027] Take 15kg of adenosylcobalamin mycelium powder (containing 0.029kg of adenosylcobalamin), put it into a solvent extraction tank, and add acetone to compound Solvent (acetone (v): isopropanol (v): water (v) = 3.5:1.5:5.5), control the stirring speed at 60rpm, and carry out leaching at room temperature. After leaching for 3 hours, filter in a centrifuge and collect the filter residue. Carry out the second leaching as before, combine the two extracts, and concentrate under reduced pressure with a thin-film evaporator to obtain adenosylcobalamin concentrated solution I 39L (potency 718 μg / ml), with a yield of 96.6%.

[0028] Put the above adenosylcobalamin concentrated solution I into the macroporous resin adsorption column, and then use 30% (v / v), 40% (v / v), 50% (v / v) acetone aqueous solution to analyze in sequence, and collect the analysis solution , concentrated under reduced pressure with a thin-film evaporator to obtain adenosylcobalamin concentrate II 75L (titer 358μg...

Embodiment 2

[0032] Take 15kg of adenosylcobalamin mycelium powder (containing 0.029kg of adenosylcobalamin), put it into a solvent extraction tank, and add acetone to compound Solvent (acetone (v): isopropanol (v): water (v) = 4.0:1.7:6.0), control the stirring speed at 60rpm, carry out leaching at room temperature, after leaching for 3 hours, filter in a centrifuge, collect the filter residue, Carry out the second leaching as before, combine the two extracts, concentrate under reduced pressure to obtain 43 L of extract concentrate, the concentration of the concentrate is 660 μg / ml, and the yield is 97.9%.

[0033] Put the above adenosylcobalamin concentrated solution I into the macroporous resin adsorption column, and then use 30% (v / v), 40% (v / v), 50% (v / v) acetone aqueous solution to analyze in sequence, and collect the analysis solution , concentrated under reduced pressure with a thin film evaporator to obtain adenosylcobalamin concentrate II 79L (titer 350μg / ml), yield 97.4%.

[00...

Embodiment 3

[0037]Take 15kg of adenosylcobalamin mycelium powder (containing 0.029kg of adenosylcobalamin), put it into a solvent extraction tank, and add acetone to compound Solvent (acetone (v): isopropanol (v): water (v) = 4.5:2.0:6.5), control the stirring speed at 60rpm, carry out leaching at room temperature, after leaching for 3 hours, filter in a centrifuge, collect the filter residue, Carry out the second leaching as before, combine the three leaching liquids, and concentrate under reduced pressure to obtain 45 L of leaching concentrated liquid, the concentration of the concentrated liquid is 630 μg / ml, and the yield is 97.8%.

[0038] Put the above adenosylcobalamin concentrated solution I into the macroporous resin adsorption column, and then use 30% (v / v), 40% (v / v), 50% (v / v) acetone aqueous solution to analyze in sequence, and collect the analysis solution , concentrated under reduced pressure with a thin-film evaporator to obtain adenosylcobalamin concentrate II 81L (titer ...

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Abstract

The invention relates to a preparation method of a cobamamide crude product. A process comprises the following steps: carrying out spray drying on pseudomonas denitrificans fermentation liquor to prepare cobamamide mycelium powder, performing extraction by using an acetone composite solvent, performing centrifugal separating and concentrating under reduced pressure to obtain a cobamamide concentrated solution I, performing adsorbing by using macroporous resin, performing desorbing by using an acetone aqueous solution, performing concentrating under reduced pressure to obtain a cobamamide concentrated solution II, and finally, carrying out flocculation filtration, acetone crystallization, suction filtration and vacuum drying to obtain the cobamamide crude product. According to the invention, the pseudomonas denitrificans fermentation liquor is subjected to the spray drying to obtain the cobamamide mycelium powder; the acetone composite solvent is used for the extraction, so that the situation that when the pseudomonas denitrificans fermentation liquor is abnormal and plate-frame filtration is difficult is avoided, cobamamide is recycled to the maximum extent, and the product qualityand yield are improved. The method has the characteristics that: the process operation is simpler and more convenient, the production schedule is easy to guarantee, the production operation coherenceis high, the generated wastewater amount is small, and the like.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation extraction, in particular to a method for preparing crude adenosylcobalamin. Background technique [0002] Adenosylcobalamin is a vitamin B 12 One of these, commonly known as coenzyme vitamin B 12 (5,6-Dimethylimidazolyl-5-deoxyadenosylcobalamin). [0003] Adenosylcobalamin participates in methyl conversion and folic acid metabolism in the body, and promotes the reduction of methyl folic acid to tetrahydrofolate; it also participates in the tricarboxylic acid cycle, which is very important for the formation of lipoproteins in nerve myelin, and can make thiol enzymes in an active state , so as to participate in a wide range of protein and fat metabolism, promote the development and maturation of red blood cells, and are necessary factors for the complete formation of nerve sheaths and spinal cord fibers and to maintain the function of epithelial cells in the digestive system. Ade...

Claims

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Application Information

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IPC IPC(8): C07H23/00C07H1/06
CPCC07H23/00C07H1/06
Inventor 户鹏东裴立忠高宏伟沈毅马琼
Owner NINGXIA KINGVIT PHARMA
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