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Neuroprotective material, multi-layer nerve repair catheter and preparation methods of nerve protection material and multi-layer nerve repair catheter

A nerve protection and catheter technology, applied in the fields of medical science, prosthesis, tissue regeneration, etc., can solve problems such as the inability to better bionic the extracellular matrix environment, and achieve the effects of preventing collapse, ensuring internal and external exchange, and strong operability

Active Publication Date: 2020-11-20
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above scheme still cannot better biomimetic extracellular matrix environment

Method used

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  • Neuroprotective material, multi-layer nerve repair catheter and preparation methods of nerve protection material and multi-layer nerve repair catheter
  • Neuroprotective material, multi-layer nerve repair catheter and preparation methods of nerve protection material and multi-layer nerve repair catheter
  • Neuroprotective material, multi-layer nerve repair catheter and preparation methods of nerve protection material and multi-layer nerve repair catheter

Examples

Experimental program
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Embodiment 1

[0056] The preparation of embodiment 1 neuroprotective material

[0057] In this example, a neuroprotective material was prepared, and the material was prepared by the following method:

[0058] Step 1: the type I collagen extracted from the bovine Achilles tendon, and the chitosan with a degree of deacetylation of 80%-95% and a viscosity of 50-800mPa·S are ground into powder respectively for subsequent use;

[0059] Step 2: dissolving type I collagen and chitosan simultaneously with an acetic acid solution with a molar concentration of 0.5M to obtain a mixed slurry, and adjusting the total concentration of the mixed slurry to be 80-130 mg / ml;

[0060] Step 3: Put the mixed slurry in a refrigerator at 4°C for 24-48 hours to fully swell the mixed slurry;

[0061] Step 4: The swollen mixed slurry is made into a neuroprotective film with a thickness of 0.1-1.0mm by a scraper;

[0062] Step 5: Freeze-dry the neuroprotective membrane using the following procedure:

[0063]

...

Embodiment 2

[0081] The preparation of embodiment 2 layered nerve guide

[0082] This embodiment provides a multilayered nerve guide, which is prepared by the following method:

[0083] Step a: pour the mixed slurry in step 3 of Example 1 into the mold of the catheter, freeze-dry to make the catheter; dissolve polylactic acid with a molecular weight of 100k-200kDa in trifluoroacetic acid to prepare a spinning solution, and electrostatically Spinning to form a nanofiber membrane, wherein the electrospinning conditions are: electrostatic high voltage 20kV, receiving distance 20cm, advancing speed 1.8ml / h, spinning time 90min; vacuum dry the nanofiber membrane at 40°C for 24h, and cut it into size A nanofiber sheet of (5-10)mm×(5-10mm);

[0084]Step b: Dissolve type I collagen with 0.05M acetic acid to a collagen concentration of 5mg / ml, add the cut nanofiber sheet, and the mass ratio of the nanofiber sheet to collagen is 0.5:1, and heat it in a homogenizer at 13000r. p.m. mix homogeneously...

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Abstract

The present application provides a neuroprotective material. The surface and interior of the neuroprotective material are porous structures, the average pores of the porous structures are less than 20microns, and at least part of pores distributed in the surface of the neuroprotective material are open holes; and the neuroprotective material is prepared from an acid mixed solution of collagen andchitosan. The present invention also provides a multi-layered nerve repair catheter, an outer layer is prepared from the neuroprotective material and an inner layer is a sponge-like nanofiber structure. The provided neuroprotective material has the more suitable porous structures, is conducive to entry of oxygen and small molecular nutrients, ensures internal and external exchanges of the nutrients, and also blocks growth of fibroblasts to avoid affecting damaged nerve cells.

Description

technical field [0001] The application relates to the technical field of tissue engineering and biomanufacturing materials, in particular to a nerve protection material, a multilayer nerve repair catheter and a preparation method thereof. Background technique [0002] In clinical treatment, the anastomosis and non-substantial damage of the nerve defect are very prone to growth of fibroblasts to cause tissue hyperplasia, which hinders the regeneration and recovery of nerve cells. [0003] The shape, metabolism, function, migration, proliferation and differentiation of cells depend on their extracellular matrix environment. The average diameter of human cells is between 10-20 μm, and studies have shown that when the pores are less than 10 μm, they are semi-permeable, only Allows red blood cells, oxygen, and small molecule nutrients to pass through. Therefore, the appropriate pore size is the main factor that can ensure the internal and external exchange of nutrients and effec...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/20A61L27/24A61L27/50A61L27/56
CPCA61L27/24A61L27/20A61L27/18A61L27/56A61L27/50A61L2430/32C08L5/08C08L67/04
Inventor 陈刚杨洪阳栗国贝孙冰冰
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD