Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

P. denitrificans/S. oneidensis/graphene/calcium alginate denitrifying gel microsphere as well as preparation method and application thereof

A technology for denitrifying Paracoccus and Shewanella, applied in biochemical equipment and methods, chemical instruments and methods, microorganism-based methods, etc., can solve problems such as accumulation of intermediate products, achieve recycling, reduce accumulation, Strong impact resistance to toxic and harmful environments

Pending Publication Date: 2020-12-08
TONGJI UNIV
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, nitrate pollution is usually accompanied by petroleum, volatile phenols, and heavy metals. According to literature reports, organic pollutants represented by phenols and heavy metals represented by uranium will have different degrees of microbial denitrification. Inhibitory effect, and will cause a large amount of accumulation of intermediate products nitrite and nitrous oxide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • P. denitrificans/S. oneidensis/graphene/calcium alginate denitrifying gel microsphere as well as preparation method and application thereof
  • P. denitrificans/S. oneidensis/graphene/calcium alginate denitrifying gel microsphere as well as preparation method and application thereof
  • P. denitrificans/S. oneidensis/graphene/calcium alginate denitrifying gel microsphere as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029]Under aerobic conditions, denitrifying microorganisms P.denitrificans (American type culture collection, strain number ATCC 19367, the same below) and Shewanella S.oneidensis MR were respectively cultivated with sterilized LB under aerobic conditions. -1 (American type culture collection bank, the strain number is ATCC 700550, the same below) to the stable period of microbial growth, centrifuged to collect and resuspend to obtain OD 600 P. denitrificans bacterial liquid and S. oneidensisMR-1 bacterial liquid both of 3; ultrasonically dispersing graphene into distilled water to obtain graphene dispersion;

[0030] The container used for the cultivation of the denitrification system is a sterile anaerobic bottle, and the denitrification medium contains the following components: 21.36mM KNO 3 ,10.66mM NH 4 Cl, 0.41mM MgSO 4 ·7H 2 O,17.93mM KH 2 PO 4 ,32.76 mM Na 2 HPO 4 , 3.00mM Lactate, 0.2g / L yeast extract, trace elements 1mL / L; among them, each 1mL trace elements ...

Embodiment 2

[0048] Preparation of denitrifying gel microspheres:

[0049] Preparation of denitrifying gel microspheres PSG: the OD 600 P.denitrificans bacteria solution and S.oneidensis MR-1 bacteria solution both of 3 were added to the graphene dispersion, mixed evenly to obtain a mixed solution, and the OD of P.denitrificans in the obtained mixed solution 600 3, the OD of P.denitrificans and S.oneidensis MR-1 600 The ratio is 5:1, and the graphene concentration is 0.1mg / mL; then mix the mixed solution with an equal volume of sodium alginate solution and add it dropwise to an appropriate amount of CaCl 2 solution, wherein the concentration of sodium alginate solution is 20g / L, CaCl 2 The mass concentration of the solution was 0.5% (w / v), and the target P.denitrificans / S.oneidensis MR-1 / graphene / calcium alginate denitrification gel microsphere PSG was obtained after stirring and reacting at room temperature for 2 hours.

[0050] Preparation of denitrification gel microspheres PS: P.den...

Embodiment 3

[0065] Preparation of denitrifying gel microspheres:

[0066] Preparation of denitrifying gel microspheres PSG: the OD 600 P.denitrificans bacteria solution and S.oneidensis MR-1 bacteria solution both of 3 were added to the graphene dispersion, mixed evenly to obtain a mixed solution, and the OD of P.denitrificans in the obtained mixed solution 600 3, the OD of P.denitrificans and S.oneidensis MR-1 600 The ratio is 1:5, and the graphene concentration is 1mg / mL; then mix the mixed solution with an equal volume of sodium alginate solution and add it dropwise to an appropriate amount of CaCl 2 In the solution, wherein, the concentration of sodium alginate solution is 40g / L, CaCl 2 The mass concentration of the solution was 4% (w / v), and the target P.denitrificans / S.oneidensis MR-1 / graphene / calcium alginate denitrification gel microsphere PSG was obtained after stirring and reacting at room temperature for 2 hours.

[0067] Preparation of denitrification gel microspheres PS: P...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses P. denitrificans / S. oneidensis / graphene / calcium alginate denitrifying gel microsphere as well as preparation method and application thereof, and belongs to the technical fieldof environmental protection. According to the invention, calcium alginate is adopted to embed denitrifying microorganisms P. denitrifians, S. oneidensis MR-1 and graphene to form P. denitrifians / S. oneidensis MR-1 / graphene / calcium alginate denitrifying gel microspheres, and the prepared microspheres have strong resistance to toxic and harmful environmental impact, and can realize recycling of strains and materials. When the microsphere is applied to an anaerobic denitrification system polluted by nitrate or co-polluted by nitrate, the time required by reaching 90% of nitrate removal rate can be greatly shortened, and the accumulation of intermediate products nitrite and nitrous oxide is reduced.

Description

technical field [0001] The invention relates to denitrifying Paracoccus / Shewanella / graphene / calcium alginate denitrification gel microspheres and a preparation method and application thereof, belonging to the technical field of environmental protection. Background technique [0002] In the past few decades, with the intensification of human activities and the large-scale use of nitrogen-containing fertilizers, a large amount of bioavailable nitrogen, such as nitrate, has inevitably entered into the environment. As an important part of the global nitrogen cycle process, microbial anaerobic denitrification, which reduces nitrate in soil or water to nitrogen gas, provides an effective way for the removal and transformation of nitrate in the environment. However, the actual biological anaerobic denitrification process is slow, and there will be some accumulation of intermediate products in the nitrate reduction process, such as nitrite and nitrous oxide. The existence of nitrit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N11/14C12N11/10C12N11/04C02F3/28C02F3/34C12R1/01C02F101/34C02F101/20
CPCC02F3/2806C02F3/34C02F2101/20C02F2101/345C12N11/04C12N11/10C12N11/14
Inventor 陈银广蒋萌董磊
Owner TONGJI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com