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Cellulose-degrading biocontrol bacillus and application and preparation thereof

A technology for degrading cellulose and Bacillus, applied in the field of microorganisms, can solve the problem of inseparable from high-efficiency cellulose-degrading strains

Active Publication Date: 2021-01-15
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, no matter which of the above-mentioned degradation methods is inseparable from the high-efficiency cellulose-degrading strains

Method used

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  • Cellulose-degrading biocontrol bacillus and application and preparation thereof
  • Cellulose-degrading biocontrol bacillus and application and preparation thereof
  • Cellulose-degrading biocontrol bacillus and application and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Isolation and Screening of Cellulose Degrading Bacteria

[0022] 1. Source of soil samples

[0023] Fresh soil samples were taken from the straw returning soil of Caoxinzhuang Experimental Farm of Northwest Agriculture and Forestry University in Yangling District, Shaanxi Province. Appropriate amount of water, for a period of one week of cultivation, until the microorganisms in the soil recover. After the microorganisms in the soil recovered, wheat and corn stalks were added to the tissue culture bottle at 28°C for enrichment culture for 50 days. Soil samples were collected on days 1, 14, and 40 to isolate cellulose-degrading bacteria.

[0024] 2. Culture medium formula

[0025] (1) Enrichment medium: 5 g of cellulose (straw), 5 g of peptone, 5 g of sodium chloride, 0.5 g of dipotassium hydrogen phosphate, 0.5 g of magnesium sulfate, and 1000 ml of distilled water. Sterilize at 121°C for 2h.

[0026] (2) Screening and identification medium: sodium carboxymethylcellu...

Embodiment 2

[0035] Identification of Cellulose Degrading Strains

[0036] After cultivating the strain in a shaker at 140rpm in liquid medium for 2 days, extract the DNA and perform 16s rDNA sequencing. The sequence results are as follows:

[0037]CTCAGTACCCTATTCCTGAGATGATTCTTGAGGAGTCCACCAAAAACTTGCTCCCTGATGTTAGCGGCGG ACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGG TTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGC TAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACT GAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAAC GCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGC GGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAA GCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAA CCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAAT GCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGT GG...

Embodiment 3

[0041] Determination of cellulase activity

[0042] 1. Drawing of glucose standard curve

[0043] Add 1mg / mL standard glucose solution 0mL, 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1mL to each of 3 tubes (3 repetitions) in 18 15ml tubes, and make up to 100mL with distilled water. 1.5ml, add 1.5mL DNS solution, shake well, place in boiling water bath and continue to heat for 10min, take it out and cool immediately, make up to 15mL accurately with distilled water, shake well, measure its absorbance at 540nm wavelength, take glucose content (mg / m L) is the abscissa, and the corresponding absorbance is the ordinate, and the glucose standard curve is drawn as Figure 4 .

[0044] 2. Determination of cellulase activity

[0045] After the crude enzyme solution is centrifuged at 12000r / min, take 0.5mL of the supernatant and add it to a stoppered colorimetric tube that has been dried to constant weight in advance, and accurately add 1mL of 1% carboxymethylcellulose sodium solution, and add Boi...

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Abstract

The invention discloses cellulose-degrading biocontrol bacillus and application and a preparation thereof. The preservation number of the cellulose-degrading biocontrol bacillus in China General Microbiological Culture Collection Center (CGMCC) is CGMCC No:19895. The strain can efficiently and quickly degrade cellulose in straws and shorten the decomposition period of the straws; and the strain can antagonize pathogenic bacteria, and after the strain is prepared into a microbial agent, crops can be protected from being invaded by the pathogenic bacteria.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a cellulosic-degrading biocontrol bacillus and its application and preparation. Background technique [0002] It is reported that my country's annual output of straw ranks first in the world. Straw is rich in organic matter and nutrients necessary for crop growth such as nitrogen, phosphorus, potassium, magnesium, calcium, and sulfur. It is of great significance to promote the growth of crops and the efficient use of nutrient resources by fully utilizing the positive effect of returning straw to the field. . At present, most straws are burned or discarded in the open air, which not only wastes resources, but also causes serious pollution to the environment. Studies have shown that long-term straw returning to the field can increase soil organic matter and improve soil physical and chemical properties. And it can reduce the pollution caused by agricultural production and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C05F11/08C05F17/20C09K17/14C12R1/07C09K101/00
CPCC12N1/20A01N63/22C05F11/08C05F17/20C09K17/14C09K2101/00C12R2001/07C12N1/205Y02W30/40
Inventor 史鹏罗慧张晶晶张志英张建丽韦革宏
Owner NORTHWEST A & F UNIV
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