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A kind of large-scale preparation method of marine source type I collagen subunit

A type I and collagen technology, which is applied in the field of large-scale preparation of marine source type I collagen subunits, can solve problems such as inability to realize industrial production, and achieve the effects of easy process control, low production cost, and clear amino acid sequence

Active Publication Date: 2022-06-03
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these preparation methods can only obtain milligram-level and microgram-level collagen subunit samples, and cannot achieve industrial production.

Method used

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  • A kind of large-scale preparation method of marine source type I collagen subunit
  • A kind of large-scale preparation method of marine source type I collagen subunit
  • A kind of large-scale preparation method of marine source type I collagen subunit

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Experimental program
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Effect test

Embodiment 1

[0034] like figure 1 As shown, a large-scale preparation method of marine source type I collagen subunit, comprising the following steps:

[0035] (1) 100kg of tilapia fish scales / fish skin / fish swim bladder are put into the cleaning tank, add 1000L sodium bicarbonate solution, the mass volume concentration of the solution is 3%, stir at room temperature for 5h, after the water is washed, add in the reactor, Add 0.5M acetic acid solution to stir and extract, and after the extract is removed by a 10,000 rpm centrifuge, the obtained clear liquid is the solution of type I collagen crude extract. After adding 3% (W / V) sodium chloride to the type I collagen crude extract solution for salting out, and centrifuging at 10,000 rpm in a centrifuge, a precipitate was obtained. After adding 0.5M acetic acid solution to dissolve the precipitate, use electrodialysis technology to remove acid and inorganic salts. Sodium solution, electrodialysis flow≤1m 3 / h, to obtain type I collagen sol...

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Abstract

The invention discloses a large-scale preparation method of type I collagen subunits from marine sources. The by-products of marine biological processing are used as raw materials, and type I collagen is prepared by using the integrated technology of acetic acid extraction, salting-out, electrodialysis and purification; type I collagen is used as raw material , the mixed solution of type I collagen subunits α1 and α2 was prepared by collagenase enzymatic hydrolysis combined with multi-stage membrane separation linkage technology; on this basis, the type I collagen subunits α1 and α2 were prepared by salting out combined with electrodialysis technology. In view of the current preparation process of collagen subunits, which can only prepare milligram-level and microgram-level samples, and cannot realize the technical bottleneck of large-scale production, the present invention constructs collagenase enzymatic hydrolysis synergistic multi-stage membrane separation linkage technology and salting-out synergistic electrodialysis technology integration A technical system for large-scale preparation of type I collagen subunits α1 and α2.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a large-scale preparation method of marine source type I collagen subunits. Background technique [0002] Collagen is the most abundant protein in vertebrates and the most important structural protein in animal skin and connective tissue, accounting for about 30% of the total protein. There are currently 29 types of collagen identified, including collagen types I-XXIX, and each type of collagen has a unique amino acid sequence, structure and function. Among all collagen types, type I collagen has good biocompatibility, biodegradability and weak antigenicity, and can provide a good source of raw materials for industries such as food, medicine, biofunctional materials and tissue engineering. [0003] In recent years, due to the occurrence of infectious diseases such as avian influenza, mad cow disease, and foot-and-mouth disease, the global demand for typ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
CPCC12P21/06C07K14/78Y02A20/124
Inventor 陈俊德高然
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES