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Glutamic acid fermentation method

A fermentation method, glutamic acid technology, applied in the field of glutamic acid fermentation, can solve the problems of acid production rate and conversion rate decline, high free ammonia toxicity, weakened respiration, etc., to reduce osmotic pressure and improve bacterial growth Effects of environment, promotion of acidogenesis and glutamate efflux

Active Publication Date: 2021-02-05
MEIHUA BIOTECH LANGFANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The process of adding the osmotic pressure regulating substance betaine to the fermentation medium can relieve the inhibition of cell respiration and the decline of cell viability under hyperosmotic conditions, but it cannot change the hypertonic environment in which the cells are located. , the osmotic pressure continues to rise in the later stage, the vitality of the bacteria continues to decline, and the respiration is weakened. At the same time, the bacteria need to continuously synthesize trehalose and proline, the osmotic pressure regulating substances, resulting in a continuous decline in the acid production rate and conversion rate in the later stage.
Moreover, the process of adding betaine from an external source cannot solve the toxicity of high free ammonia to cells

Method used

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Experimental program
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Effect test

Embodiment 1

[0058] The present embodiment provides a method for improving glutamic acid fermentation acid production and conversion rate, comprising the steps of:

[0059] (1) Seed medium preparation: glucose 40g / L, corn steep liquor 30g / L, soybean meal hydrolyzate 15g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O 1.2g / L, VB 1 100μg / L, biotin 200μg / L, FeSO 4 ·7H 2 O5mg / L, MnSO 4 ·H 2 O 5mg / L, succinic acid 1.5g / L, use sodium hydroxide to adjust the pH of the medium to 7.0-7.2, and sterilize at 121°C for 20min;

[0060] (2) Seed culture: the strain is thermosensitive Corynebacterium glutamicum for production. The strain stored in the glycerol tube was first spread on LB medium for activation and cultured overnight. Put them into a constant temperature reciprocating shaker in a 5L Erlenmeyer flask with a base, rotate at 120rpm, and temperature 32°C. Cultivate for 8-12h until the OD of the seed solution reaches 20 or above, then stop the cultivation.

[0061] (3) Fermentation medium preparati...

Embodiment 2

[0066] The present embodiment provides a method for improving glutamic acid fermentation acid production and conversion rate, comprising the steps of:

[0067] (1) Seed medium preparation: glucose 40g / L, corn steep liquor 30g / L, soybean meal hydrolyzate 15g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O 1.2g / L, VB 1 100μg / L, biotin 200μg / L, FeSO 4 ·7H 2 O5mg / L, MnSO 4 ·H 2 O 5mg / L, succinic acid 1.5g / L, use sodium hydroxide to adjust the pH of the medium to 7.0-7.2, and sterilize at 121°C for 20min;

[0068] (2) Seed culture: the strain is thermosensitive Corynebacterium glutamicum for production. The strain stored in the glycerol tube was first spread on LB medium for activation and cultured overnight. Put them into a constant temperature reciprocating shaker in a 5L Erlenmeyer flask with a base, rotate at 120rpm, and temperature 32°C. Cultivate for 8-12h until the OD of the seed solution reaches 20 or above, then stop the cultivation.

[0069] (3) Fermentation medium preparati...

Embodiment 3

[0074] The present embodiment provides a method for improving glutamic acid fermentation acid production and conversion rate, comprising the steps of:

[0075] (1) Seed medium preparation: glucose 40g / L, corn steep liquor 30g / L, soybean meal hydrolyzate 15g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O 1.2g / L, VB 1 100μg / L, biotin 200μg / L, FeSO 4 ·7H 2 O5mg / L, MnSO 4 ·H 2 O 5mg / L, succinic acid 1.5g / L, use sodium hydroxide to adjust the pH of the medium to 7.0-7.2, and sterilize at 121°C for 20min;

[0076] (2) Seed culture: the strain is thermosensitive Corynebacterium glutamicum for production. The strain stored in the glycerol tube was first spread on LB medium for activation and cultured overnight. Put them into a constant temperature reciprocating shaker in a 5L Erlenmeyer flask with a base, rotate at 120rpm, and temperature 32°C. Cultivate for 8-12h until the OD of the seed solution reaches 20 or above, then stop the cultivation.

[0077] (3) Fermentation medium preparati...

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Abstract

The invention relates to the technical field of glutamic acid fermentation, in particular to a glutamic acid fermentation method. The glutamic acid fermentation method comprises the following steps: inoculating a fermentation medium with a temperature-sensitive corynebacterium glutamicum seed solution for fermentation culture, controlling the fermentation temperature and the fermentation dissolvedoxygen (DO) value in the fermentation process, and feeding ammonia water, wherein the feeding rate of the ammonia water is adjusted according to the pH value; when the OD of fermentation liquor is smaller than or equal to 100, the pH value is 6.7; and when the OD of the fermentation liquor is larger than 100, the pH value is 7.0. Ammonium ions are replaced by high-valence cations, so that the osmotic pressure is reduced fundamentally, and the thallus growth environment is improved; a segmented pH control process is adopted, the pH is controlled to be lower in the thallus growth period, the concentration of free ammonia in fermentation liquor is reduced, the toxicity of the free ammonia to cells is reduced, and thallus growth is improved; and in the acid production period, the pH value iscontrolled to be higher, and acid production and glutamic acid discharge are promoted.

Description

technical field [0001] The invention relates to the technical field of glutamic acid fermentation, in particular to a glutamic acid fermentation method. Background technique [0002] Glutamic acid, also known as α-aminoglutaric acid, contains one amino group and two carboxyl groups in the molecule, and is an acidic amino acid. The molecular weight is 147, and the isoelectric point is 3.22. It is slightly soluble in water, but easily soluble in acidic solution. It exists in a large amount in cereal protein, and the content in animal brain is also relatively large. Glutamic acid is one of the basic amino acids for nitrogen metabolism in living organisms, participates in the synthesis of proteins, polypeptides and fatty acids, and regulates ammonia levels in the body together with glutamine; it can also participate in information transmission as an excitatory neurotransmitter. Glutamic acid has a left-handed body, a right-handed body and a racemic body. The L-body is L-glutam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14C12R1/15
CPCC12P13/14
Inventor 李必金李岩洪铭李栋梁陈琛宫仔龙
Owner MEIHUA BIOTECH LANGFANG CO LTD
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