Compound olerafuran A in purslane and its extraction and separation method and use

A separation method and compound technology, which are applied in the directions of food ingredients, applications, and drug combinations containing natural extracts, can solve problems such as low structural novelty, and achieve the effects of environmental protection process methods, simple and fast operation methods

Active Publication Date: 2022-05-13
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the chemical components isolated from purslane are known, and the structural novelty is low. Therefore, the development and isolation of new compounds in purslane are urgently needed

Method used

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  • Compound olerafuran A in purslane and its extraction and separation method and use
  • Compound olerafuran A in purslane and its extraction and separation method and use
  • Compound olerafuran A in purslane and its extraction and separation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 The compound Olerafuran A extracted and separated from purslane and its extraction and separation method A kind of compound Olerafuran A extracted and separated from purslane, the molecular formula is C 18 h 30 o 5 , named as OlerafuranA, the chemical structural formula is:

[0034] , the new compound is called Olerafuran A according to the structure, and Table 1 is the NMR data of the new compound: 1 H-NMR with 13 C-NMR in DMSO.

[0035] Table 1. NMR data of the new compounds of the present invention.

[0036] .

[0037] Olerafuran A: yellow oil, easily soluble in methanol, slightly soluble in chloroform. UV(MeOH)λmax : 213 nm. IR(KBr) Vmax 3404, 2983, 2928, 2854, 1763, 1736, 1468, 1443, 1378, 1252, 1178, 768cm-1. HR-ESI(+)-TOF-MS gave m / z: 327.2168, [M+H] + The quasi-molecular ion peak has a molecular weight of 327.2167. combine 1 H-NMR, 13 According to C-NMR and DEPT data, it is speculated that the possible molecular formula of the compound ...

Embodiment 2

[0046] Example 2 Anticholinesterase effect of compounds of the present invention.

[0047] 1. Main materials.

[0048] 1.1. Drugs and reagents.

[0049] The compound Olerafuran A used in the experiment was prepared by the above method with a purity of 90-99%. Sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.), physostigmine (Hanxiang Biotechnology), phosphorus-5,5 '-Dithiobisnitrobenzoic acid (Dithiobisnitrobenzoic acid, DTNB, Shanghai Jinsui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and iodide thioacetylcholine (Acetylthiocholine iodide, ATCI, Dalian Meilun Biotechnology Co., Ltd).

[0050] 1.2 Grouping.

[0051] Divided into negative control group, positive control group and experimental group, each group.

[0052] 2 Experimental methods.

[0053] 2.1 Sample preparation.

[0054] Precisely weigh 1 mg of samples Olerafuran A and physostigmine, respectively, and use methanol as solvent to prepare five gradient conc...

Embodiment 3

[0061] Example 3 Antitumor effect of the new compound of the present invention.

[0062] 1 main material.

[0063] 1.1 Drugs and reagents.

[0064] The new compound Olerafuran A used in the experiment was prepared by the above method with a purity of 90-99%. It was accurately weighed and diluted with DMSO to the solution required for each dosage group below. DMEM high glucose medium, fetal bovine serum (Hyclone Company, USA); penicillin, streptomycin (Hangzhou Sijiqing Company).

[0065] 1.2 Cell lines.

[0066] Human colon cancer cell Caco-2, human breast cancer cell MCF-7, human gastric cancer cell BGC-823, human lung adenocarcinoma cell SPC-A1, human liver cancer cell BEL-7402, human cervical cancer cell Hela-229, ovarian cancer cell Ho-8910, human oral epidermoid carcinoma cells KB (Shanghai Cell Bank, Chinese Academy of Sciences).

[0067] 1.3 Grouping.

[0068] Divided into control group, experimental group and zero adjustment group (culture solution containing DMSO...

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Abstract

The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a new compound extracted, separated and identified from purslane medicinal material and its extraction and separation method and application. The invention provides a compound Olerafuran A extracted from purslane and its extraction and separation method and application. The ester compound is sequentially extracted by alcohol decoction, silica gel column chromatography, polyamide column chromatography, and ODS medium pressure column. and Sephadex LH-20 purification, liquid phase separation and preparation, the extraction and separation method is simple, fast, and environmentally friendly, and the compound obtained by this method is relatively high in purity, and pharmacological experiments have proved that the obtained compound has anti-tumor and anti-cholinesterase effects Therefore, the compound Olerafuran A extracted from purslane and its salts and derivatives can be used as natural products to develop new traditional Chinese medicines, and have broad medical application prospects.

Description

technical field [0001] The present invention relates to the field of extraction and separation of traditional Chinese medicine, in particular to a new compound extracted, separated and identified from purslane medicinal material and its extraction and separation method and application, specifically a compound Olerafuran A in purslane and its extraction and separation method and uses. Background technique [0002] Purslane (Portulaca oleracea L.), also known as longevity dish, horse amaranth, is a plant of the family Amaranthaceae. Purslane is drought and waterlogging resistant, light and shade resistant, widely distributed, and rich in resources. It has attracted much attention as a wild plant for both medicine and food. The 2015 edition of "Pharmacopia of the People's Republic of China" recorded the dry aerial parts of purslane as medicine. , has the functions of clearing away heat and detoxification, cooling blood to stop bleeding, and stopping dysentery. [0003] Modern...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/60A61P35/00A61P21/04A61P27/06A61P25/28A23L33/105A23L33/10
CPCC07D307/60A61P35/00A61P21/04A61P27/06A61P25/28A23L33/105A23L33/10A23V2002/00A23V2200/30A23V2200/308A23V2250/21
Inventor 英锡相刘锡龙兰秀娟
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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