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Method for extracting flavonoid compounds from alternanthera philoxeroides

A technology of flavonoids and lotus seedlings, which is applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of long extraction period, difficult to guarantee quality stability, and cost, and achieve the effect of eliminating the harm of plant ecological environment

Inactive Publication Date: 2021-03-05
长江绿银(无锡)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has the following disadvantages: using dry grass as raw material, fresh grass is not suitable for this method; the extraction cycle is long, often takes tens of hours; the extracted flavonoids include flavonoids and their glycoside derivatives, the purity is low, and the quality is low. It is difficult to guarantee the stability of

Method used

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  • Method for extracting flavonoid compounds from alternanthera philoxeroides
  • Method for extracting flavonoid compounds from alternanthera philoxeroides
  • Method for extracting flavonoid compounds from alternanthera philoxeroides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-6

[0039] The flavonoids were extracted from A. japonicus by the following method:

[0040] Step 1) collection and cleaning of A. chinensis: collect fresh A. chinensis plants from nature reserves, wash away impurities such as silt with water, and remove surface water;

[0041] Step 2) squeezing: putting the cleaned fresh Asteroides spinosa plant obtained in step 1) into a squeezing device for squeezing to obtain squeezed juice and solid slag. The step 2) squeezing device includes a commercially available pair of roller presses, The parameters such as the diameter of the press roll, the pressure between the rolls and the pressing temperature are shown in Table 1;

[0042] Step 3) Juicing and impurity removal: Microfiltration and filtration of the squeezed juice obtained in step 2) to obtain the juice filtrate, then use petroleum ether extraction to remove chlorophyll impurities in the juice filtrate, and then use alcohol precipitation to remove the juice filtrate Polysaccharides ...

Embodiment 7-8

[0071] The basic operating parameters adopted in Examples 7-8 are the same as in Example 5.

[0072] Embodiment 7-8 In step 2) the pressing step is carried out under the protection of inert gas nitrogen, and the pressing device is arranged in a closed space, which is filled with inert gas, and keeps the closed space with a slight positive pressure to prevent oxygen in the outside air Enter the enclosed space.

[0073] The gas pressure in the closed space of Example 7 is 1000 Pa higher than the atmospheric pressure. Because the oxidation degree of the flavonoids is reduced, the purity of the extracted flavonoids reaches 88.7%, which is 5.3 percentage points higher than the result of Example 5.

[0074] Before the fresh Nitonia chinensis plant of Example 8 enters the closed space where the pressing device is located, it is purged with an inert gas, and the gas pressure in the closed space is 2000 Pa higher than the atmospheric pressure. Because the oxidation degree of the flav...

Embodiment 8-9

[0076] The basic operating parameters adopted in Examples 8-9 are the same as those in Example 3.

[0077] After step 2) of embodiment 8-9, the water washing step is added to wash out the squeezed juice remaining in the plant gap, so as to improve the yield.

[0078] The solid-to-liquid ratio of the water washing in Example 8 is 1:3, the temperature is 28°C, and the soaking time of water washing is 20 minutes. After completion, filter with a 200-mesh nylon filter cloth, and squeeze to remove the water in the solid gap. Because the water-soluble flavonoid glycosides in the plant space are recovered, the yield of the extracted flavonoids reaches 81.1%, which is 6.2 percentage points higher than the result of Example 3.

[0079] The solid-to-liquid ratio of the water washing in Example 9 was 1:7, the temperature was 16°C, and the washing soaking time was 50 minutes. After completion, filter with a 100-mesh nylon filter cloth, and squeeze to remove the water in the solid gap. Bec...

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Abstract

The invention discloses a method for extracting flavonoid compounds from alternanthera philoxeroides, and particularly relates to the field of biological separation of natural products. The method comprises the steps of collection and cleaning of the alternanthera philoxeroides, squeezing, juicing and impurity removal, concentration, alcohol extraction, alcohol removal of alcohol extract, catalytic hydrolysis, concentration and crystallization and the like, and can further comprise the steps of washing after squeezing, low-temperature gas explosion, extraction and the like, and corresponding biological enzymes can be adopted for catalytic hydrolysis. By adopting the method, the flavonoid compounds with high purity can be extracted from fresh plants of the alternanthera philoxeroides. The harm of the alternanthera philoxeroides to the natural environment is eliminated, the flavonoid compounds with anti-tumor activity, anti-virus, anti-bacterial and anti-oxidation capabilities and the like can be obtained, the defects that in the prior art, only a mixture of the flavonoid compounds and flavonoid glycoside substances can be extracted from dry plants, and the flavonoid compounds are not high in purity and quality are overcome, and two purposes are achieved through one method.

Description

technical field [0001] The invention relates to the field of biological separation of natural products, and more specifically, the invention relates to a method for extracting flavonoids from Asteroides spinosa. Background technique [0002] Lotus seedlings are perennial herbaceous plants of the Amaranthaceae lotus seedlings family, also known as hollow amaranths, philodendrons, and water peanuts. Originally produced in Brazil, after being introduced in China, it becomes wild and widely grows in ponds and ditches. With strong reproductive ability, it can become a dominant plant in a certain subtropical and temperate zone, and endanger the biodiversity of the region. It has been listed as a preliminary list of invasive alien species in China by the Ministry of Ecology and Environmental Protection. [0003] The whole lotus root can be used as medicine, which has antiviral, antibacterial, and liver protection effects. Which contains a higher concentration of flavonoids and th...

Claims

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Application Information

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IPC IPC(8): A61K36/21A61P35/00A61P31/12A61P31/04A61P39/06A61P31/10
CPCA61K36/21A61K2236/13A61K2236/31A61K2236/333A61K2236/35A61K2236/51A61K2236/53A61K2236/55A61P31/04A61P31/10A61P31/12A61P35/00A61P39/06
Inventor 王辛
Owner 长江绿银(无锡)科技有限公司
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