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Near-infrared two-zone light-emitting long afterglow nanoprobe based on X-ray excitation, preparation method and application of nanoprobe in living body imaging analysis

A nano-probe and near-infrared technology, which is applied in the use of wave/particle radiation for material analysis, analysis of materials, luminescent materials, etc. Deep in vivo tissue imaging analysis, controllable shape and size, and the effect of reducing background signal and noise

Active Publication Date: 2021-03-12
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional high-temperature solid-phase synthesis of long-lasting luminescent nanoprobes has disadvantages such as difficult preparation, low luminous efficiency, poor dispersion, and difficult to control the size and shape, and there is currently a lack of near-infrared two-dimensional probes with good penetration ability into deep living tissues. District Chief Afterglow Probe

Method used

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  • Near-infrared two-zone light-emitting long afterglow nanoprobe based on X-ray excitation, preparation method and application of nanoprobe in living body imaging analysis
  • Near-infrared two-zone light-emitting long afterglow nanoprobe based on X-ray excitation, preparation method and application of nanoprobe in living body imaging analysis
  • Near-infrared two-zone light-emitting long afterglow nanoprobe based on X-ray excitation, preparation method and application of nanoprobe in living body imaging analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Lanthanide long ginseng fluorescent probe NALU 0.8 Fly 4 : GD 0.15 / Er 0.5 @Naluf 4 Synthesis:

[0049] Step 1: First, 0.4 mmol Lu (CH 3 CO 2 ) 3 , 0.075 mmol GD (CH 3 CO 2 ) 3 , 0.025 mmol er (CH 3 CO 2 ) 3 The rare earth salt was mixed and added to 50 ml of the second-necked bottle containing 5 mL oleic acid (OA) and 7.5 mL octadene (ODE). The mixture was heated to 150 ° C for 30 min. After cooling to room temperature, it will contain 0.05 g naOH and 0.075 GNH. 4 F of the methanol solution of F added to the reaction solution, warmed to 50 ° C to continue to react 30 min. The mixed solution was continued to 100 ° C, and the solution was degassed for 10 min to remove excess methanol. Re-charge n 2 The protection was further heated to 300 ° C for 60 min. After stirring for stirring to room temperature, the centrifugation was washed multiple times with ethanol and cyclohexane, and the final core nano product NALUF was obtained. 4 : GD / ER is dispersed in 4 mL of cyclohexane...

Embodiment 2

[0053] Preparation of X-rays excited near infrared second zone emission tuned nanoparticles.

[0054] Lanthanide long ginseng fluorescent probe NALU 0.8 Fly 4 : GD 0.19 / Ln 0.01 @Naluf 4 Synthesis:

[0055] Step 1: First, 0.4 mmol Lu (CH 3 CO 2 ) 3 , 0.095 mmol GD (CH 3 CO 2 ) 3 , 0.005 mmol ln (CH 3 CO 2 ) 3 Rare earth salt mix (LN optional ND 3+ HO 3+ , Er 3+ TM 3+ , Or yb 3+ Add to 50 ml of the second-necked bottle containing 5 ml OA and 7.5 ml ODE. The mixture was heated to 150 ° C for 30 min. After cooling to room temperature, it will contain 0.05 gnoh and 0.075 g of NH. 4 F of the methanol solution of F added to the reaction solution, warmed to 50 ° C to continue to react 30 min. The mixed solution was continued to 100 ° C, and the solution was degassed for 10 min to remove excess methanol. Re-charge n 2 The protection was further heated to 300 ° C for 60 min. After stirring for stirring to room temperature, the centrifine was washed multiple times with ethanol and cyclohex...

Embodiment 3

[0059] Construction of Long Hui Biobi Image Analysis Technology in Near - infrared Second Regions.

[0060] Step 1: First take Nalu dispersed in cyclohexane 0.8 Fly 4 : GD0.15 / Er 0.5 @Naluf 4 Long Hui nanoparticle solution 1 mL (about 20 mg / ml) was added to 5 mL in chloroform solution containing 80 mg poly (maleic anhydride-Alt-1-octadene) (PMH), stirred 6 h, then nitrogen blowing Dry chloroform solution. 4-dimethylaminopyridine DMAP (80 mg) dissolved in 6 mL aqueous solution was added to the flask, and the ultrasound was detected to NALU. 0.8 Fly 4 : GD 0.15 / Er 0.5 @Naluf 4 Long Hui nano particles are completely dispersed. The solution 14000 rpm was centrifuged for 1 hour, washed twice with deionized water, remove excess PMH and DMAP. The front body was hovered in 3 mL of 4-morphinethanesulfonic acid (MES) solution (10 mM, pH = 8.5). Polyethylene glycol amino group (MPEG-NH 2 5 mg (5 kDa) and octadable amino amino (8arm-peg-NH) 2 1 mg (40 kDa) was added to 2 mL MES solution...

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Abstract

The invention relates to the field of near-infrared two-zone living body imaging in biomedical detection, in particular to a near-infrared two-zone light-emitting long afterglow nanoprobe based on X-ray excitation, a preparation method and application of the nanoprobe in living body imaging analysis. The method comprises the following steps of firstly, synthesizing a lanthanide long afterglow fluorescent probe NaLu0.8F4:Gd0.2-x / LnX@NaReF4 based on near-infrared two-zone light emitting excited by X rays, then exciting the nanoparticles to emit near-infrared two-zone fluorescence by using X-rays, and obtaining 900-1600 nm emission-adjustable near-infrared two-zone fluorescence and long afterglow light emitting by changing the types and contents of rare earth ion doping elements and adjustingthe coating type of a shell layer. The lanthanide series long afterglow nanoparticles can be used as a biocompatible probe with higher tissue penetration depth for imaging analysis of living tissuesof mice, and the analysis technology has the advantages of high sensitivity, high resolution and high signal-to-noise ratio.

Description

Technical field [0001] The present invention relates to the field of near-infrared second zone in biomedical detection, and in particular, therefore, a method of producing a long-influenza-mine probe, a preparation method, a preparation method thereof, and its application thereof in a living imaging analysis based on X-ray excited. Background technique [0002] Malignant tumors have seriously threatened people's health and survival, and there is a significant increase in the disease rate of cancer in my country in recent years. Interventional treatment is a minimally invasive treatment using modern high-tech methods, how accurately removes lesions in tumor surgery and reducing resection of patient healthy cells is a key problem. Therefore, it is important to develop clinically assistance techniques to reduce patient tumor recurrence and improve their five-year survival rate. Long Hui Luminescent probe can effectively overcome biofluorescence in biological analysis, excitation lig...

Claims

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Application Information

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IPC IPC(8): C09K11/85G01N23/223
CPCC09K11/7773G01N23/223G01N2223/076G01N2223/1016G01N2223/406G01N2223/612
Inventor 杨黄浩洪中柱陈秋水
Owner FUZHOU UNIVERSITY
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