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Microbial agent for promoting wheat growth and wheat breeding method thereof

The technology of a microbial inoculum and a composite microbial inoculant, which is applied in the field of agricultural microorganisms, can solve the problems of low functional effect, difficult adhesion, and too small seeds, and achieve the effects of shortening the emergence time, improving alkali resistance, and improving the emergence rate.

Inactive Publication Date: 2021-03-12
GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has its own defects, such as: the number of microorganisms in the epidermis is limited, some seeds are too small or difficult to attach, and the functional effects that can be exerted are greatly reduced; the inoculated microorganisms often compete with indigenous microorganisms, and the carbon source and nitrogen Competing for energy sources, snatching for ecological niches, and competing for living space, while indigenous bacteria are more competitive, and inoculum bacteria will decrease and disappear; exogenous microorganisms are affected by agricultural operations, and the use of fertilizers and drugs in farming is relatively Frequently, chemical fertilizers can destroy the microbial environment of the soil, and pesticides can kill exogenous microorganisms in the soil
[0003] The wheat varieties currently on the market are the result of artificial domestication through such a model for thousands of years, and the domestication of wheat may lead to a reduction in the types and numbers of endophytic beneficial bacteria
In the process of domesticating and breeding wheat, people focused on the characteristics of high yield, disease resistance, strong adaptability, high nutrient efficiency, water saving, and stress resistance, while ignoring the endogenous beneficial bacteria of other wheat and its related species. Lost some potentially good traits

Method used

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  • Microbial agent for promoting wheat growth and wheat breeding method thereof
  • Microbial agent for promoting wheat growth and wheat breeding method thereof
  • Microbial agent for promoting wheat growth and wheat breeding method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation of embodiment 1 microbial bacterial agent of the present invention

[0017] (1) Cultivation of strains

[0018] Pseudomonas A: Inoculate Pseudomonas fluorescens into NYBD liquid medium and culture on a shaker at 28°C for 48 hours to obtain a bacterial suspension, and then expand the culture until the bacterial concentration is not less than 10 6 / mL to obtain a secondary culture. 1L medium contains 8g of beef extract, 5g of yeast extract powder, and 10g of glucose.

[0019] B. Cultivation of Burkholderia: Inoculate Burkholderia sucrose into liquid medium and culture at 28°C for 48 hours to obtain a bacterial suspension, and then expand the culture until the bacterial concentration is not less than 10 6 / mL to obtain a secondary culture. 1 L medium contains 55 g of glucose, 15 g of magnesium sulfate heptahydrate, 15 g of ammonium sulfate, 20 g of calcium carbonate, 0.5 g of potassium dihydrogen phosphate, 10 g of peptone, and 5 g of sodium chloride. ...

Embodiment 2

[0021] Embodiment 2 The method for colonization of microbial inoculum agent of the present invention in wheat body

[0022] The present embodiment provides a method for the colonization of a compound microbial bacterial agent in wheat, and the specific steps are as follows:

[0023] The inoculant prepared in Example 1 was placed in a sprayer, and the inoculum was sprayed on the ears of Zhengmai 7698, and sprayed on the ears of wheat for five consecutive days at the exposed tissue of one-third of the spikelets; The bacterial liquid is 680 μg / μl-3500 μg / μl per spikelet. The bacteria sprayed on the leaves, stems, base and soil should be cleared in time to avoid interference. Cover the part below the ears of wheat to prevent the bacteria from falling on the leaves, stems, bases and soil of the wheat, and also prevent the interference and impact of spraying on other wheat plants.

[0024] The wheat is divided into a control group and a treatment group. The control group does no...

Embodiment 3

[0027] Embodiment 3 The impact of microbial inoculant of the present invention on wheat germination

[0028] The wheat grains obtained by the control group and the treatment group in Example 2 were dehydrated in an oven, and then selected another piece of land that was not treated with bacterial powder for planting. During planting, 10 kg / mu of nitrogen fertilizer, 2 kg of phosphate fertilizer, and 2 kg / mu of potash fertilizer were used. Mu, zinc sulfate 1 kg / mu, borax 0.2 kg / mu. Observe the germination of the seeds in the control group and the treatment group. The results are shown in Table 1:

[0029] Table 1

[0030] Group (n=20) 3 days 4 days 5 days 6 days control group 0 6 2 12 treatment group 15 4 1 0

[0031] As can be seen from Table 1, compared with the control group, the emergence time of the treatment group wheat accounted for 75% in 3 days, and the wheat of the control group mostly germinated after 6 days, illustrating t...

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Abstract

The invention provides a microbial agent for promoting wheat growth, the microbial agent is composed of fermentation broth of pseudomonas and burkholderia, and the weight part ratio of pseudomonas toburkholderia is 1:1-5. The microbial agent provided by the invention can be planted in wheat, and also has the effects of improving the alkali resistance of wheat, shortening the emergence time of wheat and improving the emergence rate.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a microbial agent for promoting wheat growth and a method for wheat breeding thereof. Background technique [0002] Wheat is the third largest food crop in my country. However, the current management of wheat cultivation in my country is highly dependent on chemical fertilizers, pesticides and plant growth regulators, while ignoring the function of beneficial endophytes. Seed inoculation is to attach microorganisms to the surface of seeds by soaking, smearing, painting, adsorption, and adhesion. This method can prevent and control plant diseases from scratch. However, this method has its own defects, such as: the number of microorganisms in the epidermis is limited, some seeds are too small or difficult to attach, and the functional effects that can be exerted are greatly reduced; the inoculated microorganisms often compete with indigenous microorg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G7/06A01G22/20A01N63/27A01N63/20A01P21/00C12R1/39C12R1/38C12R1/01
CPCA01G7/06A01G22/20A01N63/20A01N63/27C12N1/20
Inventor 李华一杨效帆罗钰彬沈家葆
Owner GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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