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OplopanaxelatusNakai chlorogenic acid extract and preparation method and application thereof

A technology of ginseng and extract, which is applied in the field of medicine, can solve the problems of poor therapeutic effect, strict and complicated use time of N-acetylcysteine, and achieve the reduction of levels, reduction of mitochondrial oxidative stress response, and alleviation of Effect of MDA content

Active Publication Date: 2021-03-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment method for acute liver injury caused by overdose of acetaminophen is mainly N-acetylcysteine, because the mechanism of liver injury caused by acetaminophen is relatively complicated, and the best treatment method of N-acetylcysteine The use time is relatively strict, generally within 8 hours, the curative effect is better, the longer the acetaminophen poisoning time, the worse the therapeutic effect
Moreover, the dosage of N-acetylcysteine ​​​​is relatively strict, and once it is used in excess, it will cause adverse reactions such as coughing, bronchospasm, nausea, vomiting, and gastritis

Method used

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  • OplopanaxelatusNakai chlorogenic acid extract and preparation method and application thereof
  • OplopanaxelatusNakai chlorogenic acid extract and preparation method and application thereof
  • OplopanaxelatusNakai chlorogenic acid extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: The preparation process of the chlorogenic acid extract from the adventitious root of Radix Ginseng in the present invention

[0048] (1) Cultivation of the adventitious roots of Northeast thorn ginseng: After the adventitious roots of the proliferation culture were cut into 1cm size, 20g (fresh weight) of the adventitious roots of the proliferation culture was weighed and inoculated in a 5L balloon bioreactor containing 4L liquid medium. MS+IBA3.0 mg / L+sucrose 50g / L, pH 5.8, ventilation adjusted to 100mL / min, dark culture at 25°C; after 30 days of culture, the adventitious roots of Panax ginseng were harvested as experimental materials.

[0049] (2) Preparation of the adventitious root extract of Northeast thorn ginseng: Soak the dry product of Northeast thorn ginseng adventitious root obtained in step (1) of Example 1 in 75% ethanol by volume (solid-liquid ratio=1:10g / mL), 60 After condensing and refluxing at ℃ for 1 hour, suction filtration was performe...

Embodiment 2

[0051] Embodiment 2: The preparation process of the chlorogenic acid extract from the adventitious root of Radix Ginseng in the present invention

[0052] (1) Cultivation of adventitious roots of Northeast thorn ginseng: After cutting the adventitious roots of proliferation culture into 1cm size, weigh 40g (fresh weight) of the adventitious roots of proliferation culture and inoculate them in a 5L balloon bioreactor containing 4L liquid medium. MS+IBA 6.0mg / L+sucrose 60g / L, pH 6.8, ventilation adjusted to 125mL / min, cultured in dark at a temperature of 30°C, harvested after 40 days of cultivation Adventitious roots of Northeast thorns were used as experimental materials.

[0053] (2) Preparation of the adventitious root extract of Northeast ginseng ginseng: Soak the dry product of Northeast thorn ginseng adventitious root obtained in step (1) of Example 2 in ethanol with a volume percentage concentration of 95% (solid-liquid ratio=1:20g / mL), After condensing and refluxing at 6...

Embodiment 3

[0055] Example 3: Study on the Prevention of Liver Injury Caused by Acetaminophen by Chlorogenic Acids Extract from Adventitious Root of Northeast Thorny Ginseng

[0056] 1. Experimental materials

[0057] The mice were pretreated for 7 days with the chlorogenic acid extract of Northeast ginseng ginseng prepared in Example 1, and the hepatoprotective effect of the chlorogenic acid extract of Northeast thorn ginseng was investigated through a control experiment.

[0058] 2. Experimental method

[0059] Set blank control group and drug treatment group respectively (low dose group 25mg·mL -1 , middle dose group 50mg·mL -1 , high dose group 100mg·mL -1 ) for 7 consecutive days, and acetaminophen solution (300 mg / kg) was injected intraperitoneally, and the mice were sacrificed 12 hours later, and serum and liver samples were preserved. Use kits to detect alanine aminotransferase and aspartate aminotransferase in serum, and determine the effect of chlorogenic acid extracts of No...

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Abstract

The invention discloses an oplopanaxelatusNakai chlorogenic acid extract and a preparation method and application thereof. The method comprises the steps that an adventitious root material of oplopanaxelatusNakai is obtained by combining a tissue culture means and utilizing a bioreactor, and the chlorogenic acid extract of oplopanaxelatusNakai is obtained through macroporous adsorption resin extraction and separation. The adventitious root chlorogenic acid extract of oplopanaxelatusNakai can significantly protect liver injury caused by acetaminophen, the liver necrosis degree is obviously reduced, the levels of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase can be significantly reduced, mitochondrial oxidative stress reaction caused by liver injury caused by excessive acetaminophen can be reduced, the activity of superoxide dismutase, catalase and glutathione in the liver is obviously restored, the content of malondialdehyde is reduced, the drug metabolism of acetaminophen in the liver is affected, and therefore the aseptic inflammatory injury of neutral granulocytes to the liver is reduced, the liver is protected, and liver injury is reduced.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a chlorogenic acid extract of Northeast thorn ginseng and its preparation method and application. Background technique [0002] Northeast thorn ginseng (Oplopanaxelatus Nakai), a plant of the genus ginseng of the Araliaceae family, is one of the unique plant resources of Changbai Mountain in my country. It is a second-class wild protected plant in the country. Volatile oil, etc., also known as "wooden ginseng" in Changbai Mountains. At present, most of the literature reports on Northeast ginseng are medicinal components and pharmacological studies, and there are relatively few clinical applications. Therefore, further effective development and utilization of its resources is of great significance. And because the wild resource of Northeast thorny ginseng is few, artificial cultivation is also unstable at present, has brought difficulty for the resource development of Northeast...

Claims

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Application Information

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IPC IPC(8): A61K36/25A61P1/16A61P31/04A61P29/00A61P39/06
CPCA61K36/25A61K2236/10A61K2236/333A61K2236/55A61P1/16A61P29/00A61P31/04A61P39/06
Inventor 廉哲雄赵志斌蒋晓龙
Owner SOUTH CHINA UNIV OF TECH
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