A kind of Solanaceae fruit vegetables and monocotyledonous crops foliar bacterial fertilizer and preparation method and application
A technology of monocotyledons and bacterial fertilizers, applied in the fields of application, nitrogen fertilizer, magnesium fertilizer, etc., can solve the problems of excessive accumulation of elements, soil physical and chemical properties, crop quality decline, crop nutritional imbalance, etc., to achieve the increase of soluble solids content and total acid concentration Enhance and promote the effect of crop growth
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Embodiment 1
[0053] Embodiment 1: a kind of foliar bacterial fertilizer of Solanaceae fruit vegetables and monocotyledonous crops
[0054] The invention provides a foliar bacterial fertilizer for Solanaceae fruit vegetables and monocotyledonous crops, which is specifically prepared by the following steps:
[0055] (1) Using KH 2 PO 4 0.5g, K 2 HPO 4 0.3g, NH 4 Cl 0.1g, MgCl 2 ﹒ 6H 2 O 0.2g, CaCl 2 ﹒ 2H 2 O0.5g, CH 3 COONa 1g, CH 3 CH 2 COONa 0.5g, yeast powder 0.1g, trace element SL-4 stock solution 1ml, liquid medium prepared with pH 5.0; trace element SL-4 stock solution composition (g / L): EDTA 0.5g, FeSO 4 ﹒ 7H 2 O 0.2g, ZnSO 4 ﹒ 7H 2 O0.01g,MnCl 2 ﹒ 4H 2 O 0.003g, boric acid 0.03g, cobalt chloride hexahydrate 0.02g, copper chloride dihydrate 0.001g, nickel chloride hexahydrate 0.002g, sodium molybdate dihydrate 0.003g, deionized water 1L;
[0056] (2) adding Rhodoblastus acidophila PNSB-AC CGMCC No.20881 strain seed liquid to the liquid medium prepared in step (...
Embodiment 2
[0058] Example 2: Isolation and Characterization of Acidophilic Photosynthetic Bacteria
[0059] (1) Isolation of acidophilic photosynthetic bacteria
[0060] The strains were isolated from water samples from the Meihua monosodium glutamate acid wastewater discharge ditch in Wujiaqu City, Xinjiang, and the pH of the wastewater was 4.5, and the sediments were collected. Targeted enrichment of purple non-sulfur acidophilic photosynthetic bacteria by enrichment culture. Enrichment medium composition (1g / L): KH 2 PO 4 0.04g, NH 4 Cl 0.1g, MgCl 2 ﹒ 6H 2 O 0.01g, CaCl 2 ﹒ 2H 2 O 0.05g, CH 3 COONa 1g, trace element SL-4 stock solution 1ml, multivitamin stock solution 1ml, pH 5.0, 25°C, light intensity 2000lx, constant temperature culture for 7 days, after the medium color turns red, suck the bacteria to isolate the bacteria. Bacteria isolation medium (RCVBN) (g / L): KH 2 PO 4 0.5g, K 2 HPO 4 0.3g, NH 4 Clg, MgCl 2 ﹒ 6H 2 O 0.2g, CaCl 2 ﹒ 2H 2 O 0.5g, CH 3 COO...
Embodiment 3
[0069] Embodiment three: a kind of preparation of the foliar bacterial fertilizer of Solanaceae fruit vegetables and monocotyledonous crops
[0070] Adopt KH 2 PO 4 0.5g, K 2 HPO 4 0.3g, NH 4 Cl 0.1g, MgCl 2 ﹒ 6H 2 O 0.2g, CaCl 2 ﹒ 2H 2 O 0.5g, CH 3 COONa 1g, CH 3 CH 2 COONa 0.5g, yeast powder 0.1g, trace element SL-4 stock solution 1ml, liquid medium prepared at pH 5.0; Rhodoblastus acidophila PNSB-AC CGMCC No.20881 strain seed liquid was added to the liquid medium In the anaerobic culture tank, the top air of the tank is emptied by passing carbon dioxide into the air, and then sealed and placed in a light incubator for constant temperature cultivation. The fermentation temperature is 30°C, the fermentation time is 5 days, and the light intensity is 1000-2000lx; 0.5 mg / L of glycerolipid, 1 mg / L of chitosan, and 0.75 mg / L of fulvic acid were mixed uniformly to prepare a foliar bacterial fertilizer for Solanaceae fruit vegetables and monocotyledonous crops.
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